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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,955 results for: FIE

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Showing: 2341 - 2370 of 9955 results

  4. pME-mKate2-KDEL-pA

    Plasmid
    #109546
    Purpose
    Multisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence
    Use
    Zebrafish plasmids
    Available Since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109546-plasmid-map-sequence-id-223868

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  7. pDuRCC-N

    Plasmid
    #81194
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please view depositor comments below and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81194-plasmid-map-sequence-id-160426

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  12. pSLC-238

    Plasmid
    #73192
    Purpose
    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
    Depositor
    Swaine Chen
    Article
    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8
    Insert
    PrhaB-chpB (chpB )
    Expression
    Bacterial
    Promoter
    PrhaB
    Available Since
    June 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    73192-plasmid-map-sequence-id-151373

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  22. pCAG-T3-CjCAS9-pA

    Plasmid
    #131543
    Purpose
    Expresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Article
    Fujii et al Int J Mol Sci. 2017 Oct 31;18(11). pii:
    Insert
    CjCas9
    Use
    CRISPR
    Tags
    FLAG-SV40NLS, SV40NLS, and polyA
    Expression
    Mammalian
    Mutation
    Human codon-optimized
    Promoter
    CAG, T3
    Available Since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131543-plasmid-map-sequence-id-262486

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Showing: 2341 - 2370 of 9955 results