We narrowed to 3,815 results for: LYC

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pET29b-PD-H166Q

Plasmid

#110179

Purpose

Expresses propanediol dehydratase mutant H166Q in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Mutation

H166Q mutant

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD-E440Q

Plasmid

#110178

Purpose

Expresses propanediol dehydratase mutant E440Q in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Mutation

E440Q mutant

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD-C438S

Plasmid

#110177

Purpose

Expresses propanediol dehydratase mutant C438S in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Mutation

C438S mutant

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD-G817A

Plasmid

#110174

Purpose

Expresses propanediol dehydratase mutant G817A in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Mutation

G817A mutant

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMDC84-ScSEIPIN-Ndelta

Plasmid

#96985

Purpose

Express yeast SEIPIN gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

ScSEIPIN (SEI1 Budding Yeast)

Tags

6xHis and GFP

Expression

Plant

Mutation

N-terminal 39 nucleotides were deleted from yeast…

Promoter

35S

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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GST-PC-1-CTS

Plasmid

#86067

Purpose

A bacterial expression plasmid encoding polycystin-1 ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Polycystin-1 (PKD1 Human)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 4297-4303

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pJK381

Plasmid

#71573

Purpose

Produces Thermoplasma acidophilum citrate synthase, Asp317>Gly mutant (TpCS-D317G)

Depositor

T. J. Kappock

Article

Kurz et al Biochemistry. 2009 Aug 25;48(33):7878-91

Insert

citrate synthase

Expression

Bacterial

Mutation

changes aspartate-317 to glycine

Promoter

recA

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pET24a_UDP_Xase_E162

Plasmid

#73160

Purpose

Expresses in E. coli, S. pneumoniae TIGR4 UDP-Xase SP_1669 mutant E162A

Depositor

Sandra Gabelli

Article

Duong-Ly et al PLoS One. 2013 May 15;8(5):e64241. doi:

Insert

pET24a

Expression

Bacterial

Mutation

change glutamate 162 to alanine

Available Since

March 22, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-GST-CHD5-L518A/Y619E-FRAG

Plasmid

#68874

Purpose

IPTG inducible bacterial expression of CHD5 fragment

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Tags

GST

Expression

Bacterial

Mutation

331-672aa, L518A/Y619E

Promoter

tac

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only
pAC-EsaR-I70V/D91G/V220A

Plasmid

#47647

Purpose

pAC-EsaR with a208g, a272g, c659t mutations in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-I70V/D91G/V220A

Use

Synthetic Biology

Expression

Bacterial

Mutation

Changed Isoleucine 70 to Valine, Aspartic acid 91…

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW-EF1

Plasmid

#18081

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW-36aa

Plasmid

#18082

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
piHGpd/G81S

Plasmid

#1111

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glycine 81 changed to serine; regulate…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-ENO1-sgRNA1

Plasmid

#165927

Purpose

CRISPR-mediated gene perturbation (knock-out) of ENO1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

ENO1 (ENO1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-ENO1-sgRNA2

Plasmid

#165928

Purpose

CRISPR-mediated gene perturbation (knock-out) of ENO1

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

ENO1 (ENO1 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-SNRNP70-sgRNA1

Plasmid

#165945

Purpose

CRISPR-mediated gene perturbation (knock-out) of SNRNP70

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

SNRNP70 (SNRNP70 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLENTICRISPR-SNRNP70-sgRNA2

Plasmid

#165946

Purpose

CRISPR-mediated gene perturbation (knock-out) of SNRNP70

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

SNRNP70 (SNRNP70 Human)

Use

Lentiviral

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-puro-GFP-3xFLAG

Plasmid

#165951

Purpose

Overexpression of GFP.

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

GFP

Use

Lentiviral

Tags

3x-FLAG

Promoter

EF1-alpha

Availability

Academic Institutions and Nonprofits only

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pWPI-Neo-LUC7L-3xFLAG

Plasmid

#165955

Purpose

Overexpression of LUC7L.

Depositor

Vamsi Mootha

Article

Alexis A. et al Molecular Cell

Insert

LUC7L (LUC7L Human)

Use

Lentiviral

Tags

3xFLAG

Promoter

EF1-alpha

Availability

Academic Institutions and Nonprofits only

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pG20_PGDH3_TEV_mVenus_FR

Plasmid

#244962

Purpose

Plant expression of PGDH3

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Insert

pgdh3 (AT3G19480 Mustard Weed)

Tags

TEV-mVenus

Expression

Plant

Promoter

UBQ10

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pG20_PGDH3_3C_mVenus_FR

Plasmid

#244963

Purpose

Plant expression of PGDH3

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Insert

pgdh3 (AT3G19480 Mustard Weed)

Tags

3C-mVenus

Expression

Plant

Promoter

UBQ10

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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LentiGuideFUT5-neo

Plasmid

#250305

Purpose

Encodes gRNA targeting FUT5

Depositor

Jennifer Kohler

Article

Konada et al Glycobiology. 2026 Feb 26:cwag011. doi:

Insert

FUT5 gRNA (FUT5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pTCB-Sig7FcholeHA

Plasmid

#237382

Purpose

pTwist CMV BetaGlobin encoding Siglec-7-Fc with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

Siglec-7-Fc (SIGLEC7 Human)

Use

Affinity Reagent/ Antibody

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2-N∆GR-G pc5087

Plasmid

#229763

Purpose

Mammalian expression plasmid of MBD2-N∆GR. N-terminus of MBD2 lacking the glycine/arginine rich domain: aa75-96. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

N-terminus of MBD2 lacking the glycine/arginine r…

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (Delta)

Plasmid

#241113

Purpose

Expresses SARS-CoV-2 spike (Delta) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (Delta) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19R,G142D,E156G,F157del,R158del,L452R,T478K,D614…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (Alpha)

Plasmid

#241032

Purpose

Expresses SARS-CoV-2 spike (Alpha) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (Alpha) (S SARS-CoV-2)

Expression

Mammalian

Mutation

H69del,V70del,Y144del,N501Y,A570D,D614G,P681H,T71…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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yTREX-ApR

Plasmid

#177307

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Type

Empty backbone

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW21K_1Ti1

Plasmid

#177291

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and eYFP

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

eYFP

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW27K_7Ti1

Plasmid

#177294

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and LacZ

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PsacB-sacB

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW08K_0C5

Plasmid

#177280

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with CmR

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

Pcat-cat

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW15K_2G5

Plasmid

#177284

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and mTagBFP2

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

mTagBFP2

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYTRW16K_1G5

Plasmid

#177286

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn5 with GmR and eYFP

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

eYFP

Use

Synthetic Biology; Yeast expression, tn5 genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYTRW28K_0Ti1

Plasmid

#177290

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

PsacB-sacB

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET-Impact-T4gp32(L301G)

Plasmid

#228209

Purpose

Expression in BL21(DE3) of single strand DNA binding protein L301G

Depositor

Stephen Benkovic

Insert

61

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Mutation

Mutated leucine 301 to glycine (L301G), improves …

Promoter

T7

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T

Plasmid

#225062

Purpose

Expression and Nuclear-localization of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_PML1 or 2-FLAG-YFast-TEV-6xHis-CylLL S15T-P2A-Myc-CylM

Plasmid

#225061

Purpose

Expression and Plasma membrane-localization of CylM-modified CylLL-S15T in human cells

Depositor

Wilfred van der Donk

Article

Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

Insert

CylLL-S15T

Tags

Multiple tags due to polycistronic expression

Expression

Mammalian

Mutation

S15T

Promoter

CMV

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SpyTag-RBD Delta

Plasmid

#214723

Purpose

Expresses SARS-CoV-2 Delta RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

Receptor-Binding Domain (S SARS-CoV-2)

Tags

SpyTag

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)_notufA-CmEndoCOM_3C-CBD-His

Plasmid

#218198

Purpose

Bacterial expression of a His-tagged Chitin Binding fusion of Cordyceps militaris EndoCOM

Depositor

Florence Pojer

Insert

EndoCOM

Tags

3C-CBD-His

Expression

Bacterial

Mutation

codon optimized Ecoli, residues 19-315

Promoter

T7

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
B-evoSpCas9-E526K

Plasmid

#207365

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-E526K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, Y515N, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-evoSpCas9-V495M

Plasmid

#207363

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-V495M

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Y515N, K526E, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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