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SFFV-mCh-GATA2-R361L

Plasmid

#193080

Purpose

constitutive expression of mCherry fused to human Gata2 with mutation R361L in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-GATA2-R361L

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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SFFV-mCh-GATA2-R362Q

Plasmid

#193081

Purpose

constitutive expression of mCherry fused to human Gata2 with mutation R362Q in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-GATA2-R362Q

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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SFFV-mCh-GATA2-C373R

Plasmid

#193082

Purpose

constitutive expression of mCherry fused to human Gata2 with mutation C373R in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-GATA2-C373R

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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SFFV-mCh-GATA2-R396Q

Plasmid

#193083

Purpose

constitutive expression of mCherry fused to human Gata2 with mutation R396Q in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-GATA2-R396Q

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-mCh-Gata2

Plasmid

#193061

Purpose

doxycycline-inducible expression of mCherry fused to mouse Gata2 in mammalian cell

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-Gata2

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-mCh-Gfi1b

Plasmid

#193062

Purpose

doxycycline-inducible expression of mCherry fused to mouse Gfi1b in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

mCh-Gfi1b

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-Gata2-mCh

Plasmid

#193064

Purpose

constitutive expression of mouse Gata2 fused to mCherry in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

Gata2-mCh

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-Gfi1b-mCh

Plasmid

#193065

Purpose

constitutive expression of mouse Gfi1b fused to mCherry in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

Gfi1b-mCh

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mNudcd3 - 1

Plasmid

#198501

Purpose

lentiviral stable expression of mNudcd3 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mNudcd3 (Nudcd3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mNudcd3 - 2

Plasmid

#198502

Purpose

lentiviral stable expression of mNudcd3 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mNudcd3 (Nudcd3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mRab12-2

Plasmid

#198476

Purpose

lentiviral stable expression of mRab12 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mouse Rab12 (Rab12 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mRab12-1

Plasmid

#198475

Purpose

lentiviral stable expression of mRab12 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mouse Rab12 (Rab12 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-squirrel-monkey-CHMP3(155)

Plasmid

#154183

Purpose

expresses squirrel monkey CHMP3(155) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3(155)

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 155

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1827

Plasmid

#154323

Purpose

Multi-cassette for SapTrap

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::mScarlet-I

Use

CRISPR

Expression

Worm

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1841

Plasmid

#154338

Purpose

Promoter reporter

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pDK616

Plasmid

#134202

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD) mutant

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Expression

Bacterial

Mutation

K223A K226A

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pIW040

Plasmid

#134127

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Expression

Bacterial

Mutation

aa 132-255 R236A

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pIW039

Plasmid

#134126

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Expression

Bacterial

Mutation

aa 132-255 and K203A K206A

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pIW028

Plasmid

#134125

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Expression

Bacterial

Mutation

aa 132-255 and K183A K184A

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pIW070

Plasmid

#134138

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 delta MTBD CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

residues 1-131, CRISPR resistant

Available Since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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