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pRH030

Plasmid

#159633

Purpose

CRISPR‐Cas9 tool for the genetic engineering of Bacillus phages

Depositor

Robert Hertel

Article

Otte et al Lett Appl Microbiol. 2020 Jul 2. doi: 10

Type

Empty backbone

Use

CRISPR; Shuttle vector

Tags

Expression

Mutation

Promoter

Constitutive tracrRNA

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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mTagBFP-Lysosomes-20

Plasmid

#55263

Purpose

Localization: Lysosome Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

LAMP1

Use

Tags

mTagBFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pNDL-60

Plasmid

#161720

Purpose

In vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNA

Depositor

Adam Cohen

Article

McNamara et al bioRxiv [Preprint]. 2024 Apr 12:2024.04.

Insert

cytosolic acvr2b-CIB1N (acvr2b Zebrafish)

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP720

Plasmid

#45467

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Use

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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piRFP670-N1

Plasmid

#45457

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

iRFP670

Use

Tags

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP670

Plasmid

#45466

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Use

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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GST-HA-GFP11-N1

Plasmid

#91953

Purpose

Expresses GST-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

GST-HA-GFP11

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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Lifeact-Halo

Plasmid

#193328

Purpose

mammalian transient expression - expression of Lifeact-Halo

Depositor

Klaus Hahn

Article

Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

Insert

Lifeact

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-tTA

Plasmid

#104109

Purpose

AAV-mediated expression of tTA under the Syn1 promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tTA2

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn1

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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FlucWT-HA-GFP11-N1

Plasmid

#91954

Purpose

Expresses FlucWT-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucWT-HA-GFP11

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pPAmCherry-a-tubulin

Plasmid

#31930

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Use

Tags

a-tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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AiRTX_ctrl

Plasmid

#214069

Purpose

Fluorescent reporter system, NMD negative control. Intron present in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Use

Tags

Expression

Mammalian

Mutation

Remnant of linker sequence present at the end of …

Promoter

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaA

Plasmid

#50386

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.

Depositor

Erick Strauss

Article

Brand et al J Biol Chem. 2005 May 27;280(21):20185-8

Insert

PanK

Use

Tags

6XHis-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-EYFP-WPRE

Plasmid

#104111

Purpose

AAV-mediated expression of EYFP under the TRE promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

EYFP

Use

AAV

Tags

Expression

Mutation

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJKR-H-tetR

Plasmid

#62561

Purpose

Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pKM402

Plasmid

#107770

Purpose

Expression of phage Che9c RecT for oligo-mediated recombineering in mycobacteria

Depositor

Kenan Murphy

Article

Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

Inserts

Che9C RecT
TetR (tet repressor)
cat
kan

Use

Tags

Expression

Bacterial

Mutation

Promoter

Cat promoter, Ptet, kan promoter, and pTB21

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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LGmCreER (self deleting)

Plasmid

#33340

Depositor

Scott Lowe

Article

Zhao et al Genes Dev. 2010 Jul 1;24(13):1389-402.

Insert

CreERT2

Use

Cre/Lox and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-tdTomato-WPRE

Plasmid

#104112

Purpose

AAV-mediated expression of tdTomato under the TRE promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tdTomato

Use

AAV

Tags

Expression

Mutation

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRPS5A-PTP-3Xflag- psa A Left -G1397_C ABE

Plasmid

#189646

Purpose

psa A Left -G1397_C ABE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox and TadA 8e

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PalmPalm-ClopHensor

Plasmid

#25940

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

PalmPalm-ClopHensor

Use

Tags

Gap20 and Strep-II

Expression

Mammalian

Mutation

N-term (20AA) of Gap-43

Promoter

Available since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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