We narrowed to 1,706 results for: SUA

1. qTAG-C-moxGFP-Puro-H2BC11

   Plasmid

   #207758

   Purpose

   Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. qTAG-C-miRFP670nano3-Blast-H2BC11

   Plasmid

   #207762

   Purpose

   Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. qTAG-C-mScarlet-Puro-H2BC11

   Plasmid

   #207761

   Purpose

   Donor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. qTAG-C-moxGFP-Zeo-H2BC11

   Plasmid

   #207759

   Purpose

   Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pME-mScarlet3-Giantin (JDW 1356)

   Plasmid

   #224502

   Purpose

   Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mScarlet3-Giantin
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. HsB2AR-mCherry

   Plasmid

   #137785

   Purpose

   Visualization of the Beta-2-adrenergic receptor

   Depositor

   Dorus Gadella

   Article

   Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

   Insert

   Human beta2-adrenergic receptor (ADRB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-ATF6-(S1P-)

   Plasmid

   #32956

   Purpose

   For visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.

   Depositor

   Ron Prywes

   Article

   Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

   Insert

   ATF6 (ATF6 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S1Protease site mutation: R415A/R416A

   Promoter

   CMV

   Available since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-N-Solo-mStayGold-MYO1C

   Plasmid

   #227306

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. DHFR-mNeon-GluA1

   Plasmid

   #173009

   Purpose

   For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   DHFR-mNeon-GluA1
   

   Use

   Tags

   DHFR and mNeon

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin (CAG)

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTol2-CreLite

    Plasmid

    #131783

    Purpose

    Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.

    Depositor

    George Eisenhoffer

    Article

    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

    Insert

    PhyBCreC, PIF6CreN, and mTagBFP2
    

    Use

    Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Ubi

    Available since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. phage - ubc - nls - 2xmcp - egfp - BirA*

    Plasmid

    #131132

    Purpose

    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

    Depositor

    Ralf Jansen

    Article

    Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11

    Insert

    NLS-HA-2xMCP-EGFP-BirA*
    

    Use

    Lentiviral

    Tags

    NLS, HA

    Expression

    Mammalian

    Mutation

    BirA* = BIrA >R118G

    Promoter

    human ubiquitin C (UBC)

    Available since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-mScarlet-gamma9

    Plasmid

    #140097

    Purpose

    Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Insert

    GNGT2
    

    Use

    Tags

    N-terminal mScarlet tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. qTAG-C-moxGFP-Blast-H2BC11

    Plasmid

    #207757

    Purpose

    Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. qTAG-C-mNeon-Blast-TUBB4B

    Plasmid

    #207786

    Purpose

    Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pONSY-CoNMM:mCherry

    Plasmid

    #111878

    Purpose

    This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
    

    Use

    Capsaspora owczarzaki

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid

    #207760

    Purpose

    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pRD398

    Plasmid

    #163105

    Purpose

    Expression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mCherry_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pONSY-CoH2B:Venus

    Plasmid

    #111877

    Purpose

    This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Insert

    Capsaspora Histone H2B (CoH2B) fused to Venus
    

    Use

    Capsaspora owczarzaki

    Tags

    Venus

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRD397

    Plasmid

    #163104

    Purpose

    Expression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNA

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS_mTurquoise2_H-NSdbd_NLS
    

    Use

    Eukaryotic expression

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter; T7 promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF Doc2beta 6X mutant GFP

    Plasmid

    #128823

    Purpose

    To visualize Doc2beta 6X mutant in PC12 cells

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D163A, D218A, D220N, D303A, D357A, D359A

    Promoter

    EF-1alpha

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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