We narrowed to 5,570 results for: donor

1. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

   Plasmid

   #121174

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFHMSP-LIC-N

   Plasmid

   #26095

   Purpose

   Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   His and TEV cleavage site

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Sept. 15, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

   Plasmid

   #121175

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19_CDC45_dTAG

   Plasmid

   #232733

   Purpose

   Generates CDC45 dTAG C ternimal knock-in cell lines as donor

   Depositor

   Junjie Chen

   Insert

   CDC45 (CDC45 Human)
   

   Expression

   Bacterial

   Mutation

   Synonymous mutation

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19_MCM2_dTAG

   Plasmid

   #232731

   Purpose

   Generates MCM2 dTAG C ternimal knock-in cell lines as donor

   Depositor

   Junjie Chen

   Insert

   MCM2 (MCM2 Human)
   

   Expression

   Bacterial

   Mutation

   Synonymous mutation

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pINO4-NLS-Pfv-Sapphire-HO

   Plasmid

   #203660

   Purpose

   Yeast vector encoding donor DNA for integration of nuclear 40nm GEMs into the HO locus.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   CRISPR

   Tags

   Sapphire

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFHMSP-LIC-C

   Plasmid

   #26100

   Purpose

   Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   8x His tag and MKFLVNVALVFMVVYISYIYAAA (Honeybee …

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pFETCh_DNMT3B

   Plasmid

   #72365

   Purpose

   Donor vector for 3' FLAG tag of human DNMT3B

   Depositor

   Richard M. Myers, Eric Mendenhall

   Insert

   DNMT3B Homology Arms (DNMT3B Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. HR_G3BP1-V5-APEX2-GFP

   Plasmid

   #105284

   Purpose

   homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

   Depositor

   Eugene Yeo

   Article

   Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

   Insert

   G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 soybean, Human)
   

   Use

   Human homologous recombination donor

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-Neo-M2rtTA

    Plasmid

    #60843

    Purpose

    AAVS1 donor vector for genomic targeting

    Depositor

    Rudolf Jaenisch

    Article

    DeKelver et al Genome Res. 2010 Aug;20(8):1133-42. doi:

    Inserts

    M2rtTA
    Neo
    

    Use

    Targeting donor

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188747

    Purpose

    Donor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-ZNF804B

    Plasmid

    #88756

    Purpose

    Donor Vector containing ZNF804B transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ZNF804B (ZNF804B Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCL025 AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188743

    Purpose

    Donor vector for integrating RSV promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    RSV

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188744

    Purpose

    Donor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188745

    Purpose

    Donor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    nonTATAchr21

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid

    #188746

    Purpose

    Donor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    Surface marker and mCitrine reporter
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    nonTATAchrX

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221-ETV3

    Plasmid

    #88804

    Purpose

    Donor Vector containing ETV3 transcription factor, part of the Human TFome Collection

    Depositor

    Marc Vidal

    Article

    Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

    Insert

    ETV3 (ETV3 Human)
    

    Use

    Gateway donor vector

    Mutation

    Last nucleotide of stop codon removed to allow fo…

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. SOX17-tdTomato-EhB

    Plasmid

    #210467

    Purpose

    Donor plasmid for knock-in tdTomato into the human SOX17 locus

    Depositor

    Kai Wang

    Insert

    SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived hygromycin-NLS-mTagBFP2 selection cassette (SOX17 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_mCherry

    Plasmid

    #221470

    Purpose

    Gateway cloning of mCherry overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    mCherry
    

    Use

    Gateway donor vector

    Available Since

    July 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SOX9-T2A-NLS-tdTomato-PGK-Puro

    Plasmid

    #89993

    Purpose

    Donor template for generation of SOX9-ntdTomato reporter cell lines

    Depositor

    Timo Otonkoski

    Insert

    SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)
    

    Use

    CRISPR

    Promoter

    none

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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