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We narrowed to 4,861 results for: donor

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Showing: 1561 - 1580 of 4861 results

  1. B2M-SDMutation-EhT

    Plasmid
    #215546
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Publication
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived hygromycin-NLS-tdTomato selection cassette (B2M Human)
    Use
    CRISPR
    Mutation
    The second base of the first intron of B2M (From …
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215546-plasmid-map-sequence-id-425336

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  2. B2M-SDMutation-EPG

    Plasmid
    #215545
    Purpose
    Donor plasmid to introduce splice donor mutation on the first intron of human B2M locus to mediate knock-out
    Depositor
    Kai Wang
    Publication
    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do
    Insert
    B2M homologous recombination arms (Mutation on right HA) and EF1alpha-drived puromycin-GFP selection cassette (B2M Human)
    Use
    CRISPR
    Mutation
    The second base of the first intron of B2M (From …
    Available since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    215545-plasmid-map-sequence-id-425338

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  5. pSPL

    Plasmid
    #110743
    Purpose
    Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site
    Depositor
    Tim Richmond
    Publication
    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi
    Type
    Empty backbone
    Expression
    Insect
    Promoter
    Polyhedrin and p10 promoters
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    110743-plasmid-map-sequence-id-213592

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  6. LTR-[Bxb1(attP)-spacer-BceINT(attP)-spacer-Pa01(attP)-spacer-SacINT(attP)-pEF1a-Puro-LTR (DRR728)

    Plasmid
    #248232
    Purpose
    Lentiviral plasmid containing Bxb1, BceINT, Pa01, and SacINT attP target sites
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586
    Insert
    LTR-[Bxb1(attP)-spacer-BceINT(attP)-spacer-Pa01(attP)-spacer-SacINT(attP)-pEF1a-Puro-LTR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    None
    Promoter
    NA
    Available since
    April 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    248232-plasmid-map-sequence-id-506813

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  7. pCMV-bpNLS-Stab-exin21-Bxb1(A315R)-Stab (CJT605)

    Plasmid
    #248211
    Purpose
    bpNLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter.
    Depositor
    Benjamin Kleinstiver
    Publication
    Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586
    Insert
    bpNLS-Stab-exin21-Bxb1(A315R)-Stab
    Use
    CRISPR; In vitro transcription
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Bxb1(A315R)
    Promoter
    CMV and T7
    Available since
    April 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248211-plasmid-map-sequence-id-506607

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  14. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Publication
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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  16. pHW2128

    Plasmid
    #255955
    Purpose
    N-terminal TAG-IN donor plasmid for co-expression tagging with TetON = rTetR-L-QFAD (selection marker, SEC flanked by LoxP511I)
    Depositor
    Han Wang
    Insert
    FRT-SEC(pDD364)-rTetR(tet-ON, DBD)-linker-QFAD-stop(TAA)-SL2-N' linker-SD-rFRT3
    Expression
    Worm
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    255955-plasmid-map-sequence-id-511487

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  17. pHW2116

    Plasmid
    #255956
    Purpose
    N-terminal TAG-IN donor plasmid for co-expression tagging with TetOFF = TetR-L-QFAD (selection marker, SEC flanked by LoxP511I)
    Depositor
    Han Wang
    Insert
    FRT-SEC(pDD364)-tTA(tet-OFF, DBD)-linker-QFAD-stop(TAA)-SL2-N' linker-SD-rFRT3
    Expression
    Worm
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    255956-plasmid-map-sequence-id-518809

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  18. pHW2072

    Plasmid
    #255934
    Purpose
    C-terminal TAG-IN donor plasmid for co-expression tagging with TetON = rTetR-L-QFAD (selection marker, SEC flanked by LoxP511I)
    Depositor
    Han Wang
    Insert
    FRT-SA-C' linker-SL2-rTetR(tet-ON, DBD)-linker-QFAD-stop(TAA)-SEC(pDD364)-N' linker-rFRT3
    Expression
    Worm
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    255934-plasmid-map-sequence-id-511494

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  19. pHW2073

    Plasmid
    #255935
    Purpose
    C-terminal TAG-IN donor plasmid for co-expression tagging with TetOFF = TetR-L-QFAD (selection marker, SEC flanked by LoxP511I)
    Depositor
    Han Wang
    Insert
    FRT-SA-C' linker-SL2-tTA(tet-OFF,DBD)-linker-QFAD-stop(TAA)-SEC(pDD364)-N' linker-rFRT3
    Expression
    Worm
    Available since
    Sept. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    255935-plasmid-map-sequence-id-511492

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Showing: 1561 - 1580 of 4861 results