We narrowed to 4,736 results for: donor

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DDX3X - E186A in pENTR

Plasmid

#216577

Purpose

gateway cloning donor vector containing DDX3X ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway Cloning

Mutation

E186A

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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DDX3X - I179T in pENTR

Plasmid

#216576

Purpose

gateway cloning donor vector containing DDX3X ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway Cloning

Mutation

I179T

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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DDX3X - R145C in PENTR

Plasmid

#216575

Purpose

gateway cloning donor vector containing DDX3X ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway Cloning

Mutation

R145C

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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DDX3X - G123E in pENTR

Plasmid

#216574

Purpose

gateway cloning donor vector containing DDX3X ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway Cloning

Mutation

G123E

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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DDX3X - I107V in pENTR

Plasmid

#216573

Purpose

gateway cloning donor vector containing DDX3X ASD mutation

Depositor

Douglas Allan, Christopher Loewen

Insert

DDX3X (DDX3X Human)

Use

Gateway Cloning

Mutation

I107V

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pSLHDR03_mCherry_tCD19_unique guide

Plasmid

#124062

Purpose

Construction of 1st donor for scarless genome editing with unique guide

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Inserts

mCherry
tCD19

Use

Mouse Targeting; Human targeting

Promoter

Human UbC modified

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-PKCa-mEGFP-N1

Plasmid

#112269

Purpose

IDOCKSalpha Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

Protein Kinase C alpha (PRKCA Bovine)

Tags

mEGFP

Expression

Mammalian

Mutation

A11V (please see depositor comments below)

Promoter

CMV

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pAC1823_pCR8-RBFOX1N-dCasRx-C

Plasmid

#118657

Purpose

Gateway Donor; RBFOX1N-dCasRx-C ORF; No promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

RBFOX1N-dCasRx-C

Use

CRISPR

Expression

Mammalian

Promoter

No promoter

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMLS383

Plasmid

#73736

Purpose

SapTrap TEV-Flagtag N-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

TEV-FLAGtag

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS281

Plasmid

#73732

Purpose

SapTrap syntron-embedded FLP-on N-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

syntron embedded N-terminal FLP-on

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pMLS268

Plasmid

#73726

Purpose

SapTrap SL2 C-terminal connector donor plasmid

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

SL2 (gdp-2/gdp-3 intergenic region)

Use

CRISPR

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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qTAG-C-dTAG-Zeo

Plasmid

#207738

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-ultraID-Blast

Plasmid

#207715

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR-NLS-actin-R62D

Plasmid

#118381

Purpose

Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

NLS and R62D

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pT2-CAG-fGFP

Plasmid

#108714

Purpose

Transposon donor plasmid for long-term expression of membrane targetted GFP

Depositor

Madeline Lancaster

Article

Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666.

Insert

EGFP

Tags

6xHis and farnesylation signal

Expression

Mammalian

Promoter

CAG

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSLHDR02_EGFP_tCD8

Plasmid

#124061

Purpose

Construction of 1st donor for scarless genome editing

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Inserts

EGFP
tCD8

Use

Mouse Targeting; Human targeting

Promoter

Human UbC promoter modified

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-B2

Plasmid

#122580

Purpose

MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-Ah2

Plasmid

#122569

Purpose

MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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BB3nK_ext_AC

Plasmid

#108686

Purpose

Plasmid for assembly of donor DNA templates consisting of two expression units

Depositor

Brigitte Gasser

Article

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_KDM6A

Plasmid

#86265

Purpose

Donor vector for 3' FLAG tag of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KDM6A homology arms (KDM6A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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