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pXTm-PH domain

Plasmid

#210326

Purpose

Expresses EGFP with PH domain

Depositor

Li Yu

Article

Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

Insert

PH domain

Use

Fish expression

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-PT2A-Tdtomato-iRFP670-GFP

Plasmid

#111815

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

Tdtomato
iRFP670
GFP

Use

Cloning intermediate

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-PGD2-1.1

Plasmid

#214764

Purpose

AAV packaging plasmid for GRAB PGD2-1.1 fluorescent sensor under EF1alpha promoter

Depositor

Yulong Li

Article

Sang et al Cell. 2023 Dec 7;186(25):5500-5516.e21.

Insert

GRAB-PGD2-1.1 sensor

Use

AAV

Promoter

EF-1-alpha promoter

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-IDR1-mCherry-Cry2

Plasmid

#177125

Purpose

Express IDR1 fragment of YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (IDR1 domain) (YTHDC1 Human)

Tags

mCherry-CRY2

Expression

Mammalian

Mutation

IDR1 domain only

Promoter

SFFV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_g5-HT3.0mut

Plasmid

#208724

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut

Use

AAV

Promoter

EF1a

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti ABE7.10-N-AIDmax

Plasmid

#157949

Purpose

Lentiviral expression of ABE7.10-N-AIDmax

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Lenti ABE7.10-N-AIDmax

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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p415PGK1-AtMAX1(pYL759)

Plasmid

#178289

Purpose

Expresses MAX1 from A. thaliana (AtMAX1) in Saccharomyces cerevisiae. Centromeric LEU, PPGK1-AtMAX1-Tpho5

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

AtMAX1

Expression

Yeast

Promoter

PGK1

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_UCN1.0

Plasmid

#208668

Purpose

Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMX-Ascl1-puro

Plasmid

#198227

Purpose

Retroviral expression of Ascl1

Depositor

Li Qian

Article

Wang et al Cell Stem Cell. 2022 Oct 6;29(10):1491-1

Insert

Ascl1 (Ascl1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pYTSG

Plasmid

#159419

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Saccharomyces cerevisiae

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Yeast

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pmx-MiGT

Plasmid

#172397

Purpose

Retroviral expression vector for murine direct cardiac reprogramming

Depositor

Li Qian

Article

Wang et al Cells. 2020 Jan 22;9(2). pii: cells90202

Insert

Mef2c-i, Gata4, Tbx5 (Gata4, Mef2c, Tbx5 Mouse)

Use

Retroviral

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSB_init_Sb68

Plasmid

#159423

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb68

Tags

c-Myc and poly-His

Expression

Bacterial

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pXTm-zebrafish Tspan4a

Plasmid

#210327

Purpose

Expresses Tetraspanin-4a (TSPAN4a) as migrasome marker

Depositor

Li Yu

Article

Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

Insert

Tetraspanin 4a (tspan4a Zebrafish)

Use

Fish expression

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57-mcGAS-LL/RK-NLS

Plasmid

#185457

Purpose

Inducible nuclear cGAS

Depositor

Shitao Li

Article

Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

Insert

cGAS

Use

Lentiviral

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

L157R, L159K

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_g5-HT2m

Plasmid

#208722

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2m in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2m

Use

AAV

Promoter

EF1a

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-crRNA4n96

Plasmid

#123640

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

crRNA scaffold 4n96

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

Plasmid

#194750

Purpose

To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_OTmut

Plasmid

#185385

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_CCK1.0

Plasmid

#208674

Purpose

Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_NPYmut

Plasmid

#208679

Purpose

Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut

Use

AAV

Promoter

hSyn

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pSB_init-Sb66

Plasmid

#159422

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb66

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb66

Tags

c-Myc and poly-His

Expression

Bacterial

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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BPK2014-AmCas12b

Plasmid

#121951

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AmCas12b

Tags

10xHis tag

Expression

Bacterial

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_CRF1.0

Plasmid

#208662

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0

Use

AAV

Promoter

EFS

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-GaCYP722C(pYL777)

Plasmid

#178290

Purpose

Expresses GaCYP722C from G. arboreum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GaCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

GaCYP722C

Expression

Yeast

Promoter

GAP

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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p416PGK1-OsCYP711A2(pYL770)

Plasmid

#178301

Purpose

Expresses OsCYP711A2 from O. sativa in Saccharomyces cerevisiae. Centromeric URA, PPGK1-OsCYP711A2-Tpho5

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

OsCYP711A2

Expression

Yeast

Promoter

PGK1

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-ArCas12a-2AeGFP

Plasmid

#123632

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

ArCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-LpCas12a-2AeGFP

Plasmid

#123635

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

LpCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PA-DreN

Plasmid

#169707

Purpose

The optimized DreN246-nMag

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

Dre246-nMag

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PA-DreC

Plasmid

#169705

Purpose

The optimized pMag-Dre247

Depositor

Dali Li

Article

Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

Insert

pMag-Dre247

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
BPK2013-AkCas12b

Plasmid

#121950

Purpose

E. coli expression, protein purification

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AkCas12b

Tags

10xHis tag and 3xHA tag

Expression

Bacterial

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-GRAB_CRF0.5

Plasmid

#208654

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS

Plasmid

#194755

Purpose

Lentiviral expression of BioID2-HA-13 x Linker- RBP(Z) on the cell surface via a GPI anchor

Depositor

Chaoyang Li

Article

Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

Insert

PrP (1-22)-BioID2-HA-13 x Linker- RBP(Z)-GPI-PSS

Use

Lentiviral

Tags

HA

Promoter

CMV

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-U6-AagRNA

Plasmid

#121953

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b tracrRNA/crRNA duplex

Expression

Mammalian

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFTSG

Plasmid

#162389

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cell

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

His

Expression

Insect

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-BsCas12a-2AeGFP

Plasmid

#123633

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

BsCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-U6-Aa-artsg13 (BsaI)

Plasmid

#188279

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

Insert

Aa-artsg13

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-AaCas12b(R911A)-2AeGFP

Plasmid

#121961

Purpose

Mammalian expression, Transcription regulation, deactivated AaCas12b

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

Catalytically deactivated AaCas12b

Tags

3xHA tag

Expression

Mammalian

Mutation

R911A

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 Flag-Sesn1b

Plasmid

#61867

Purpose

Transient overexpression of Flag-Sesn1b (mouse)

Depositor

Ming Li

Article

Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

Insert

Sesn1 (Sesn1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
PB-BE3-2NLS

Plasmid

#119031

Purpose

Stably express BE3 in mammalian cells

Depositor

Jinsong Li

Article

Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

Insert

BE3

Tags

3xFlag

Expression

Mammalian

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-PrCas12a-2AeGFP

Plasmid

#123636

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

PrCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 281 - 320 of 32647 results

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