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Showing: 141 - 160 of 9910 results

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pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158972

Purpose

Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pVasa-Cre

Plasmid

#15885

Depositor

Diego Castrillon

Article

Gallardo et al Genesis. 2007 Jun . 45(6):413-7.

Insert

mouse vasa promoter (Ddx4 Mouse)

Use

Tags

Cre

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pCRISPR-Cre stuffer v4

Plasmid

#158033

Purpose

lenti-viral construct with Cas9, Cre recombinase and U6 driven sgRNA casette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pBOB-CAG-SARS-CoV2-Spike D614G-HA

Plasmid

#158761

Purpose

3rd Generation lentiviral vector expressing the codon optimized SARS-CoV2 Spike Glycoprotein D614G high infectivity mutant with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

Depositor

Gerald Pao

Article

Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

Insert

SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Spike D614G

Promoter

CAG

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-DPP4-blast

Plasmid

#158452

Purpose

Constitutive lentiviral expression of human DPP4.

Depositor

Alejandro Chavez, Sho Iketani

Insert

DPP4 (DPP4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158651

Purpose

making transgenics expressing a UAS driven YFP and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Tags

Expression

Mutation

F70A/F108Y

Promoter

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL4B-FLAG

Plasmid

#15822

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005 . 399():287-309.

Insert

Cullin4B (CUL4B Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N594

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG-TET3G-T2A-Puro

Plasmid

#158067

Purpose

Encodes the Tet3G transactivator and puromycin resistance.

Depositor

Adolfo Rivero-Muller

Article

Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

Insert

pCAG-TET3G-T2A-Puro

Use

AAV

Tags

puromycin reistance gene

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-TagRFP-T_A1aY1

Plasmid

#158754

Purpose

Expression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

6xHistidine_TagRFP-T_A1aY1

Use

Tags

6x-Histidine tag and TagRFP-T

Expression

Mammalian

Mutation

Promoter

CMV Promoter

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL5-FLAG

Plasmid

#15823

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005 . 399():287-309.

Insert

Cullin5 (CUL5 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N441

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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GAL FLAG103QDProGFP p306

Plasmid

#15834

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-DN-hCUL2-FLAG

Plasmid

#15819

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005 . 399():287-309.

Insert

Cullin2 (CUL2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N427

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SARS-CoV-2 M

Plasmid

#158078

Purpose

Expresses SARS-CoV-2 membrane protein in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 matrix (M )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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GAL FLAG72QDProGFP p306

Plasmid

#15833

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q Delta Pro (HTT Human)

Use

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Promoter

Available since

Oct. 5, 2007

Availability

Academic Institutions and Nonprofits only
pXPR_502_sgCD4

Plasmid

#158704

Purpose

CD4 CRISPRa control

Depositor

John Doench, David Root

Article

Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

Insert

CD4 (CD4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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TRE-mCherry-p2A-Chrimson-TS

Plasmid

#158702

Purpose

Reporter construct

Depositor

Alice Ting

Article

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

TRE-mcherry-p2A-Chrimson-TS

Use

Synthetic Biology

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR E3 pR8A L3-L2

Plasmid

#158735

Purpose

Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NN)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E3 pR5A L3-L2

Plasmid

#158732

Purpose

Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last HD)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E1 pF8A L1-L4

Plasmid

#158731

Purpose

Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NN)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR E1 pF5A L1-L4

Plasmid

#158728

Purpose

Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last HD)

Depositor

Shin-ichi Arimura

Article

Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

Insert

TALEN ORF without TAL repeat

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge