We narrowed to 10,401 results for: 158

1. pCR-Zeo

   Plasmid

   #158709

   Purpose

   For cloning and constitutive expression of crRNAs in mycobacterium

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   crRNA
   

   Use

   CRISPR and Synthetic Biology; Mycobacteria expres…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Hsp60

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDESTpK7WG2mito

   Plasmid

   #158726

   Purpose

   Plant mitoTALEN vector assembly, Destination vector

   Depositor

   Shin-ichi Arimura

   Article

   Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

   Insert

   mitochondrial presequence in front of attR1 in pk7WG2
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TwinStrep-SUMO-NLS-BhCas12b_v4

   Plasmid

   #158615

   Purpose

   For protein expression of BhCas12b_v4

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   NLS-BhCas12b_v4
   

   Use

   Tags

   6xHIS-TwinStrep-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Human IgM-Fc

   Plasmid

   #158214

   Purpose

   Express the Fc region of human IgM

   Depositor

   Junyu Xiao

   Article

   Li et al Science. 2020 Feb 28;367(6481):1014-1017

   Insert

   IGHM (IGHM Human)
   

   Use

   Tags

   twin strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPP089

   Plasmid

   #158796

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS2181

   Plasmid

   #158421

   Purpose

   AAV plasmid with Ple32 (CLDN5 MiniPromoter) driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple32 (CLDN5 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ple32

   Available since

   Feb. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pyEvolvR (enCas9-PolI5M)

   Plasmid

   #158073

   Purpose

   Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber

   Article

   Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

   Insert

   enCas9-PolI5M
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ131-tRNA2.0

   Plasmid

   #158393

   Purpose

   Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Without promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX307-TMPRSS2-blast

   Plasmid

   #158458

   Purpose

   Constitutive lentiviral expression of human TMPRSS2.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYC1640

    Plasmid

    #158719

    Purpose

    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

    Plasmid

    #158585

    Purpose

    Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    membrane localization signal-tagBFP-wrmScarlet11
    

    Use

    Tags

    tagBFP

    Expression

    Yeast

    Mutation

    GGGS linker

    Promoter

    TEF

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHTganA-Cpf1

    Plasmid

    #158647

    Purpose

    Constitutive transcription of FnCpf1 and ganA crRNA

    Depositor

    Wenjing Cui

    Insert

    Cpf1, ganA crRNA
    

    Use

    CRISPR and Synthetic Biology; Shuttle vector baci…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P43

    Available since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRDA_256

    Plasmid

    #158581

    Purpose

    Lentiviral expression of BE3.9max and customizable gRNA

    Depositor

    David Root, John Doench

    Article

    Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 D10A

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pADsacA

    Plasmid

    #158649

    Purpose

    Constitutive transcription of sacA crRNA and GFP

    Depositor

    Wenjing Cui

    Insert

    sacA crRNA
    

    Use

    CRISPR and Synthetic Biology; Shuttle vector baci…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pveg

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR E3 pR8A L3-L2

    Plasmid

    #158735

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last NN)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    TALEN ORF without TAL repeat
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR E3 pR5A L3-L2

    Plasmid

    #158732

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 3 with attL3 L2 (last HD)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    TALEN ORF without TAL repeat
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR E1 pF8A L1-L4

    Plasmid

    #158731

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last NN)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    TALEN ORF without TAL repeat
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR E1 pF5A L1-L4

    Plasmid

    #158728

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 1 with attR4 R3 (last HD)

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    TALEN ORF without TAL repeat
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR E2 HSPter-35Sp-mito

    Plasmid

    #158727

    Purpose

    Plant mitoTALEN vector assembly, Entry clone 2 with attL1 L4

    Depositor

    Shin-ichi Arimura

    Article

    Kazama et al Nat Plants. 2019 Jul;5(7):722-730. doi:

    Insert

    A terminator of Arabidopsis heat shock protein, CAMV35S promoter, and mitochondrial presequence
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. MAC-FURIN

    Plasmid

    #158386

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    furin, paired basic amino acid cleaving enzyme (FURIN Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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