We narrowed to 400 results for: PUR ALPHA-1

1. pEAT8-137 M1V

   Plasmid

   #173807

   Purpose

   Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells

   Depositor

   Anne Gershenson

   Article

   Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi

   Insert

   SERPiNA1 (SERPINA1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E1M Otherwise this plasmid encodes the most commo…

   Promoter

   T7

   Available Since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHH0103_NCK1-1/3

   Plasmid

   #91472

   Purpose

   Protein expression and purification of human SH3 domain construct NCK1-1/3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   NCK1-1/3 (NCK1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCIP-AMPKa1_WT

   Plasmid

   #79010

   Purpose

   For mammalian expression of WT AMPK alpha1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   AMPK alpha 1 (PRKAA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGs

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)

   Plasmid

   #99343

   Purpose

   Bacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TAD

   Depositor

   Peter Wright

   Article

   Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

   Inserts

   Hif1a (776-826) (HIF1A Human)
   CBP (340-439)
   

   Use

   Tags

   His6GB1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL4.20-HIF1A prom

   Plasmid

   #40173

   Depositor

   Alex Minella

   Article

   Sengupta et al Mol Cell Biol. 2011 Sep;31(18):3885-95.

   Insert

   HIF1A promoter fragment (HIF1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCIP-AMPKa1_KD

   Plasmid

   #79011

   Purpose

   For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   AMPK alpha 1 (PRKAA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47R

   Promoter

   pCAGGs

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459-HypaCas9-TUBA1B_sgRNA

   Plasmid

   #183889

   Purpose

   pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   TUBA1B sgRNA spacer (TUBA1B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459-HypaCas9-mR2-TUBA1B_sgRNA

   Plasmid

   #183888

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   TUBA1B sgRNA spacer (TUBA1B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Integrin beta1(1-455)-tev-BHCys

   Plasmid

   #27315

   Depositor

   Timothy Springer

   Article

   Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

   Insert

   integrin beta1 (ITGB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   beta1(1-455)+GG+TEV+GGKN+BHCys+TG+His6

   Promoter

   Available Since

   April 8, 2011

   Availability

   Academic Institutions and Nonprofits only

10. HisGB1-Hif1a(776-826)_FIH

    Plasmid

    #99508

    Purpose

    Bacterial expression of hydroxylated Hif1a TAD (776-826)

    Depositor

    Peter Wright

    Article

    Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

    Inserts

    Hif1a (776-826) (HIF1A Human)
    FIH1 (HIF1AN Human)
    

    Use

    Tags

    His6GB1

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW57.1 Lysosomal-METRIQ

    Plasmid

    #135401

    Purpose

    Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity

    Depositor

    Eisuke Itakura

    Article

    Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

    Insert

    DNAse II alpha (DNASE2 Human)
    

    Use

    Lentiviral

    Tags

    T2A, mCherry, and sfGFP

    Expression

    Mammalian

    Mutation

    Single-nucleotide polymorphism C432T

    Promoter

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSIN-PAmCherry-KFERQ-NE

    Plasmid

    #102365

    Purpose

    Lentiviral overexpression of PA-mCherry-KFERQ fusion

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Inserts

    PA-mCherry
    KFERQ peptide
    

    Use

    Lentiviral

    Tags

    NE

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-DAG1

    Plasmid

    #205149

    Purpose

    Overexpress DAG1

    Depositor

    Monkol Lek

    Insert

    Dystroglycan 1 (DAG1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Carries common missense variant c.41C>G (p.Ser…

    Promoter

    EF-1a

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-shDnd1-No7

    Plasmid

    #70068

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #7

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pLKO.1-shDnd1-No1

    Plasmid

    #70058

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #1

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1-shDnd1-No3

    Plasmid

    #70060

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #3

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-shDnd1-No4

    Plasmid

    #70065

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #4

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

18. pLKO.1-shDnd1-No5

    Plasmid

    #70066

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #5

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1-shDnd1-No6

    Plasmid

    #70067

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #6

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pLKO.1-shDnd1-No8

    Plasmid

    #70069

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #8

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only