We narrowed to 1,007 results for: Psp

1. pSplit-NO-Dib1

   Plasmid

   #58786

   Purpose

   Express LucN-hDib1 fusion protein in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   hDIb1
   

   Use

   Tags

   Flag and Luciferase (1-398aa)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pSPlit-OC-hDib1

   Plasmid

   #58789

   Purpose

   Express hDib1-LucC fusion protein in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   hDib1 (TXNL4A Human)
   

   Use

   Tags

   Flag and Luciferase (394-550aa)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pSplit-ON-hDib1

   Plasmid

   #58787

   Purpose

   Express hDib1-LucN fusion protein in mammalian cells

   Depositor

   Hiroyoshi Ariga

   Article

   Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

   Insert

   hDib1 (TXNL4A Human)
   

   Use

   Tags

   Flag and Luciferase (1-398aa)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pSP64T-hLbCpf1

   Plasmid

   #107187

   Purpose

   plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)

   Depositor

   Antonio Giraldez

   Article

   Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

   Insert

   hLbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mutation

   Promoter

   sp6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

   Plasmid

   #172225

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSP64TS (DM#117)

   Plasmid

   #15034

   Depositor

   Douglas Melton

   Article

   Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

   Insert

   beta-globin 5' and 3' UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

   Plasmid

   #110300

   Purpose

   Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

   Depositor

   Chris Kok-Lung Chan

   Article

   Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

   Insert

   p53-binding protein 1 (TP53BP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET30-2-PSPH

   Plasmid

   #83909

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   phosphoserine phosphatase (PSPH Human)
   

   Use

   Tags

   6x His tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

   Plasmid

   #188541

   Purpose

   Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

   Depositor

   Darrell Kotton

   Article

   Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSP72-deltaFlincG

    Plasmid

    #19733

    Depositor

    Wolfgang Dostmann

    Article

    Nausch et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    delta-FlincG (PRKG1 Bovine)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 77-356

    Promoter

    Available since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pSplit-OC-U5-40K

    Plasmid

    #51741

    Purpose

    Express U5-40K-LucC fusion protein in mammalian cells

    Depositor

    Hiroyoshi Ariga

    Article

    Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

    Insert

    U5-40K (SNRNP40 Human)
    

    Use

    Tags

    FLAG and Luciferase (394-550aa)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pSP72 GSK3beta

    Plasmid

    #60125

    Purpose

    for transcription of GSK3beta

    Depositor

    Andrew McMahon

    Insert

    Glycogen synthase kinase 3 beta (Gsk3b Mouse)
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpyTag-GlpG

    Plasmid

    #79054

    Purpose

    Expresses SpyTagged GlpG protein

    Depositor

    James Bowie

    Article

    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi

    Insert

    SpyTag-GlpG (glpG )
    

    Use

    Tags

    6xHis and SpyTag

    Expression

    Bacterial

    Mutation

    GlpG protein employed here corresponds to residue…

    Promoter

    Trc

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-PSPC1-CT

    Plasmid

    #124374

    Purpose

    expresses EGFP-PSPC1-CT131 in mammalian cell

    Depositor

    Yuh-Shan Jou

    Article

    Lang et al Nat Commun. 2019 Dec 16;10(1):5716. doi:

    Insert

    PSPC1 CT (PSPC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

    Plasmid

    #172224

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPSP6+t-gfp

    Plasmid

    #48130

    Purpose

    Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Insert

    gfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSP64T-dnWnt-11

    Plasmid

    #16857

    Depositor

    Jim Smith

    Article

    Tada et al Development. 2000 May . 127(10):2227-38.

    Insert

    dnWnt-11 (wnt11b.L Frog)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    amino acids 1-282. Dominant Negative.

    Promoter

    Available since

    May 27, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pSP64T acvr1ba*

    Plasmid

    #185409

    Purpose

    Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

    Depositor

    Nicolas David

    Article

    Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

    Insert

    acvr1ba (acvr1ba Zebrafish)
    

    Use

    In vitro synthesis of mrna

    Tags

    Expression

    Mammalian

    Mutation

    changed Threonine 206 to Aspartic acid

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. BII-CPspaH2B

    Plasmid

    #133385

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MEIS1A-pSP65

    Plasmid

    #21014

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

    Insert

    MEIS1A (Meis1 Mouse)
    

    Use

    In vitro tnt

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only