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pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)

Plasmid

#48295

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6

Expression

Insect

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV_dCas9_VP64

Plasmid

#49015

Purpose

encodes human-optimized dCas9_VP64 synthetic transcription factor

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

dCas9_VP64 (human-codon-optimized)

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pH3FS

Plasmid

#85780

Purpose

N-ICE plasmid pH3FS

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5-BMLF1

Plasmid

#72636

Purpose

Mammalian expression vector for Epstein-Barr virus Mta immediate-early post-transcriptional regulator

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BMLF1/BSLF2

Expression

Mammalian

Mutation

Q157H

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
438-GST

Plasmid

#97092

Purpose

GST-tev-yORF

Depositor

Chris Jeans

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

GST tag and TEV cleavage site

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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TagBFP2-PIP4K2C

Plasmid

#202730

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:PIP4K2C (PIP4K2C Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pK3FS

Plasmid

#85776

Purpose

N-ICE plasmid pK3FS

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pH3F-GAL1

Plasmid

#85775

Purpose

N-ICE plasmid pH3F-GAL1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBigTB

Plasmid

#149432

Purpose

shuttle vector with blasticidin-S resistance into ROSA26 targetting vectors

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Promoter

SV40

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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PCLP-2/NT-IgG (104)

Plasmid

#24423

Depositor

Steven Rosen

Article

Sassetti et al J Biol Chem. 2000 Mar 24. 275(12):9001-1

Insert

PCLP-2 (PODXL2 Human)

Tags

Human IgG1 Fc

Expression

Mammalian

Mutation

N-terminal (acidic) domain of PCLP-2 (1-630 bp) f…

Available Since

Sept. 27, 2010

Availability

Academic Institutions and Nonprofits only
pK3F

Plasmid

#85770

Purpose

N-ICE plasmid pK3F

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5-BMRF1

Plasmid

#72631

Purpose

Mammalian expression vector for Epstein-Barr virus polymerase accessory protein

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BMRF1

Tags

D22G and S349N

Expression

Mammalian

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
pK3FS-CYC1

Plasmid

#85779

Purpose

N-ICE plasmid pK3FS-CYC1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-CYC1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pH3FS-PYK1

Plasmid

#85781

Purpose

N-ICE plasmid pH3FS-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pK3F-GAL1

Plasmid

#85772

Purpose

N-ICE plasmid pK3F-GAL1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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f1H (264)

Plasmid

#24425

Depositor

Steven Rosen

Article

Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

Insert

SULF1 (SULF1 Human)

Tags

FLAG, His, and Myc

Expression

Mammalian

Mutation

22aa N-terminal truncation

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pH3F

Plasmid

#85773

Purpose

N-ICE plasmid pH3F

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODAL-matMYC

Plasmid

#115639

Purpose

For targeted integration and inducible expression of human NODAL (with an N-terminal MYC tag on the mature peptide) using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL (NODAL Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pBM4607

Plasmid

#18795

Depositor

Mark Johnston

Article

Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

Insert

Sir4 (SIR4 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

aa 951-1200

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pK3F-PYK1

Plasmid

#85771

Purpose

N-ICE plasmid pK3F-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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