We narrowed to 666 results for: Tight TRE

1. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

   Plasmid

   #102891

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo

   Plasmid

   #102892

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRetroX-Tight-puro-3xFLAG-RUNX3 R122C

   Plasmid

   #203439

   Purpose

   Mammalian expression of RUNX4

   Depositor

   Yoshiaki Ito

   Article

   Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

   Insert

   RUNX3 (RUNX3 Human)
   

   Use

   Tags

   3 x FLAG

   Expression

   Mammalian

   Mutation

   R122C

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pL(CMVtight-phi-AQP1-FKBP12DD-IRES-EGFP-WPRE)

   Plasmid

   #236278

   Purpose

   Lentiviral vector that can express doxycycline-inducible hAqp1 with fkbp12 degron in Tet ON mammalian cells. CMVtight promoter. EGFP marker.

   Depositor

   Arnab Mukherjee

   Article

   Yun et al Chem Sci. 2024 Jun 4;15(28):11108-11121.

   Insert

   human aquaporin 1 (AQP1 Human)
   

   Use

   Lentiviral

   Tags

   Flag and fkbp12

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtight

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRetroX-Tight-Pur-Trim69 isoform B (codon optimized)

   Plasmid

   #199567

   Purpose

   Stable and dox. inducible expression of Trim69 isoform B (codon optimized) after retroviral-mediated gene transfer

   Depositor

   Yuxin Song, Andrea Cimarelli

   Article

   Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

   Insert

   Trim69 isoform B (codon optimized) (TRIM69 Human)
   

   Use

   Retroviral; Allows for puromycin selection

   Tags

   Flag

   Expression

   Mutation

   codon optimized

   Promoter

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRetroX-Tight-Pur-Trim69 isoform E (codon optimized)

   Plasmid

   #199570

   Purpose

   Stable and dox. inducible expression of Trim69 isoform E (codon optimized) after retroviral-mediated gene transfer

   Depositor

   Yuxin Song, Andrea Cimarelli

   Article

   Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

   Insert

   Trim69 isoform E (codon optimized) (TRIM69 Human)
   

   Use

   Retroviral; Allows for puromycin selection

   Tags

   Flag

   Expression

   Mutation

   codon optimized

   Promoter

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRetroX-Tight-Pur-Trim69 isoform D (codon optimized)

   Plasmid

   #199569

   Purpose

   Stable and dox. inducible expression of Trim69 isoform D (codon optimized) after retroviral-mediated gene transfer

   Depositor

   Yuxin Song, Andrea Cimarelli

   Article

   Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

   Insert

   Trim69 isoform D (codon optimized) (TRIM69 Human)
   

   Use

   Retroviral; Allows for puromycin selection

   Tags

   Flag

   Expression

   Mutation

   codon optimized

   Promoter

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRetroX-Tight-Pur-Trim69 isoform C (codon optimized)

   Plasmid

   #199568

   Purpose

   Stable and dox. inducible expression of Trim69 isoform C (codon optimized) after retroviral-mediated gene transfer

   Depositor

   Yuxin Song, Andrea Cimarelli

   Article

   Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

   Insert

   Trim69 isoform C (codon optimized) (TRIM69 Human)
   

   Use

   Retroviral; Allows for puromycin selection

   Tags

   Flag

   Expression

   Mutation

   codon optimized

   Promoter

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRetroX-Tight-Pur-Trim69 Full length, isoform A (codon optimized)

   Plasmid

   #199566

   Purpose

   Stable and dox. inducible expression of Trim69 Full length, isoform A (codon optimized) after retroviral-mediated gene transfer

   Depositor

   Yuxin Song, Andrea Cimarelli

   Article

   Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

   Insert

   Trim69 Full length, isoform A (codon optimized) (TRIM69 Human)
   

   Use

   Retroviral; Allows for puromycin selection

   Tags

   Flag

   Expression

   Mutation

   codon optimized

   Promoter

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201151

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    SadCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: ATCAGTGATAGAGAACGTATG

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-dCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201152

    Purpose

    Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    dCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mutation

    gRNA sequence: TACGTTCTCTATCACTGAT

    Promoter

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLitTREPitt

    Plasmid

    #18661

    Depositor

    Jakob Reiser

    Article

    Pluta et al J Gene Med. 2005 Jun . 7(6):803-17.

    Insert

    TREPitt
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNL-EGFP/TREPittdU3

    Plasmid

    #18659

    Depositor

    Jakob Reiser

    Article

    Pluta et al J Gene Med. 2005 Jun . 7(6):803-17.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTRE ZO1myc

    Plasmid

    #30318

    Depositor

    Alan Fanning

    Article

    Fanning et al Mol Biol Cell. 2007 Mar . 18(3):721-31.

    Insert

    Zonula Occludens-1 (TJP1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pLitTREPitt

    Plasmid

    #18661

    Depositor

    Jakob Reiser

    Article

    Pluta et al J Gene Med. 2005 Jun . 7(6):803-17.

    Insert

    TREPitt
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTRE/WT SP with 5'UTR

    Plasmid

    #89330

    Purpose

    Inducible expression of WT spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-responsive P tight

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRE/SP M1WT

    Plasmid

    #89327

    Purpose

    Inducible expression of WT M1 spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-194 bp of 5'UTR deleted

    Promoter

    Tet-responsive P tight

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTRE/SP M87 WT

    Plasmid

    #89328

    Purpose

    Inducible expression of WT M87 spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-460 bp deleted

    Promoter

    Tet-responsive P tight

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTRE/SP M87C448Y

    Plasmid

    #89329

    Purpose

    Inducible expression of M87 C448Y spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-460 bp deleted, C448Y

    Promoter

    Tet-responsive P tight

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRE/SP M1C448Y

    Plasmid

    #89326

    Purpose

    Inducible expression of M1C448Y spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-194 bp of 5'UTR deleted, C448Y

    Promoter

    Tet-responsive P tight

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge