We narrowed to 2,503 results for: gem

1. Geminin-mStrawberry reporter

   Plasmid

   #158690

   Purpose

   This plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-PspCas13b-GEMSgRNA2

   Plasmid

   #204768

   Purpose

   pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   guide RNA targeting GEMS m6A sensor mRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMHE-mCherry-mTrim21

   Plasmid

   #105522

   Purpose

   T7 promotor drives in vitro transcription of mCherry-tagged wildtype mouse TRIM21 mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIS GEM-CEPIA1er

   Plasmid

   #58217

   Purpose

   Ratiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulum

   Depositor

   Masamitsu Iino

   Article

   Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

   Insert

   GEM-CEPIA1er
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   GEM-GECO1 E298D F359W D400E E407D

   Promoter

   CAG

   Available since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_GEM4-Halo-FRB

   Plasmid

   #197057

   Purpose

   Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_GEM7-Halo-FRB

   Plasmid

   #197058

   Purpose

   Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   Tags

   FRB and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEMHE-vhhGFP4-hIgG1-Fc

   Plasmid

   #105576

   Purpose

   T7 promotor drives in vitro mRNA transcription of GFP nanobody-Fc antibody domain fusion

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Human IgG1 Fc-domain fused with GFP nanobody (IGHG1 Human)
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. CD11b promoter in pGEM3zf(-)

   Plasmid

   #26168

   Depositor

   Daniel Tenen

   Article

   Dziennis et al Blood. 1995 Jan 15. 85(2):319-29.

   Insert

   CD11b promoter (ITGAM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEMHE-mH33B-mClover3

   Plasmid

   #171496

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   H3.3B (H3f3b Mouse)
   

   Use

   Pgehme

   Tags

   mClover3

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. sg_Gemin2_i1-ipUSEPR-TR657

    Plasmid

    #228919

    Purpose

    Knockdown of Gemin2 in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    sg_Gemin2_i1 (Gemin2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. sg_Gemin2_i5-ipUSEPR-TR657

    Plasmid

    #228920

    Purpose

    Knockdown of Gemin2 in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    sg_Gemin2_i5 (Gemin2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEMHE-H2B-mEGFP

    Plasmid

    #105528

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged Histon 2B mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    H2B (H2BC11 Human)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMHE-membrane-mEGFP

    Plasmid

    #105526

    Purpose

    T7 promotor drives in vitro mRNA transcription of a mEGFP-tagged membrane reporter

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    GNAI2 (GNAI2 Human)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    fragment encoding the plasma membrane targeting s…

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMHE-mEGFP-mTrim21

    Plasmid

    #105519

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-NLS-mEGFP

    Plasmid

    #105527

    Purpose

    T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Nucleoplasmin
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    NLS of nucleoplasmin (encoding amino acids KRPAAT…

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Clover-Geminin(1-110)

    Plasmid

    #83915

    Purpose

    Fluorescent probe for M/G1 transition

    Depositor

    Michael Lin

    Article

    Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    Clover-Geminin(1-110)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBiT3.1-C_GEMIN5:1-1508

    Plasmid

    #211164

    Purpose

    Mammalian expression of full-length human GEMIN5. C-terminal HiBiT tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    Gemin5:M1-M1508 (GEMIN5 Human)
    

    Use

    Tags

    GSSGGSSGVSGWRLFKKIS

    Expression

    Mammalian

    Mutation

    R682Q natural variant (VAR_033807)

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEM mouse islet1 [TJ#28]

    Plasmid

    #16270

    Depositor

    Thomas Jessell

    Insert

    islet1 (Isl1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

19. p3523 pGEM HPV-11 E6

    Plasmid

    #10854

    Depositor

    Peter Howley

    Article

    Werness et al Science. 1990 Apr 6. 248(4951):76-9.

    Insert

    HPV11 E6
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pGEMHE-mEGFP-Map4

    Plasmid

    #105571

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    MAP4 (Map4 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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