We narrowed to 1,029 results for: gibson assembly

1. pQE80L SpyTag-ELP-SpyTag

   Plasmid

   #112634

   Purpose

   Two SpyTags linked by an elastin-like polypeptide for hydrogel formation

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   SpyTag-ELP-SpyTag
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   central cysteine

   Promoter

   T5

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHIV_pTp66p51

   Plasmid

   #78233

   Purpose

   Expresses HIV reverse transcriptase

   Depositor

   Christopher Voigt

   Article

   Elbaz et al Nat Commun. 2016 Apr 19;7:11179. doi: 10

   Inserts

   p66
   p51
   LacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Const. Promoter (From DNA2.0 Vec) and pTac Minima…

   Available since

   Aug. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR8-attL5-pegRNA-attR3

   Plasmid

   #213054

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDD379

   Plasmid

   #91834

   Purpose

   SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDD121

   Plasmid

   #91833

   Purpose

   Cas9 expression in C. elegans

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-IMX-DogTag

   Plasmid

   #113765

   Purpose

   Expresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigase

   Depositor

   Mark Howarth

   Article

   Andersson et al Sci Rep. 2019 Mar 15;9(1):4625. doi: 10.

   Insert

   IMX-DogTag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQE80L TriCatcher-ELP

   Plasmid

   #112630

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-ELP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFD-NIa-Protease-TVMV

   Plasmid

   #137109

   Purpose

   iFLinkC Linker cloning

   Depositor

   Viktor Stein

   Article

   Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

   Insert

   NIa Protease TVMV
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBTK519

   Plasmid

   #110603

   Purpose

   BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance)

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBTK402

    Plasmid

    #110598

    Purpose

    BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    mRFP dropout (RSF1010 origin)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBTK501

    Plasmid

    #110602

    Purpose

    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBTK520

    Plasmid

    #110604

    Purpose

    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBTK403

    Plasmid

    #110599

    Purpose

    BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    mRFP dropout (RSF1010 origin)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBTK401

    Plasmid

    #110597

    Purpose

    BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    mRFP dropout (RSF1010 origin)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBTK601

    Plasmid

    #110607

    Purpose

    BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MZB184

    Plasmid

    #228488

    Purpose

    Empty 10xHis-SUMO (Smt3) vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    10xHis-SUMO(Smt3)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MZB223

    Plasmid

    #228491

    Purpose

    Empty C-terminal 8xHis tag vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MZB116

    Plasmid

    #228480

    Purpose

    "Empty" C-terminal self-cleaving CPD-tag vector for rapid cloning/DNA Assembly via SwaI restriction enzyme site (bacterial expression)

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    CPD-10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MZB133

    Plasmid

    #228484

    Purpose

    "Empty" N-terminal TEV cleavable 6x His tag vector for rapid cloning/DNA Assembly via SmaI restriction site (Bacterial Expression)

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. C77m

    Plasmid

    #120989

    Purpose

    MoClo golden gate assembly CD partfor AmpR (ampicillin/carbenicillin resistance protein). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    AmpR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge