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Plasmid#83377PurposeControl FRET probe for measuring tension on Talin moleculeDepositorInsertTalin1 (Tln1 Mouse)
UseRetroviralTagsEGFP-F40-tagRFP (tension sensor module)ExpressionMammalianMutationTension sensor module is inserted at C-terminal o…PromoterCMVAvailable sinceOct. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMax_BFP_A191G
Plasmid#177823PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).DepositorInserttagBFP_A191G
UseTagsExpressionMammalianMutationA191GPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AEB-No-aptamer-controlmRFP1
Plasmid#63848PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyTagsExpressionMutationPromoterJ23100Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
LVDP-CArG-RE-GPR
Plasmid#89762PurposeQuantitative measurement of CArG response element activity.DepositorInsert6X CArG-RE
UseLentiviralTagsCMV-ZsGreenExpressionMammalianMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
HP35 tension sensor
Plasmid#101250PurposeExpresses the HP35-based tension sensor module. The biosensor allows force measurements at 6-8 pN.DepositorInsertYPet(short)-HP35-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
F40 tension sensor
Plasmid#101252PurposeExpresses the F40-based tension sensor module. The biosensor allows force measurements at 1-6 pN.DepositorInsertYPet(short)-F40-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
stabilized HP35 tension sensor module
Plasmid#101251PurposeExpresses the stabilized HP35-based tension sensor module. The biosensor allows force measurements at 9-11 pN.DepositorInsertYPet(short)-HP35st-mCherry
UseRetroviralTagsHis-tag and cysteineExpressionMammalianMutationPromoterCMVAvailable sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFAB3833
Plasmid#47839PurposeBIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.DepositorInsertpromoter 8 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3737
Plasmid#47838PurposeBIOFAB RFP reporter plasmid for measuring promoter 7 + BCD1 efficiency.DepositorInsertpromoter 7 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi1-91-NLuc
Plasmid#207148PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI1 (GNAI1 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)KPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFAB3677
Plasmid#47833PurposeBIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.DepositorInsertpromoter 2 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα15-244-NLuc
Plasmid#207154PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA15 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNA15 (GNA15 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position E(244)NPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
RGD-sfGFP-I27-CC
Plasmid#85392PurposeMTFM sensor to measure integrin mechanical forces. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines (CC) for immobilization and sfGFP for fluorescent labelingDepositorInsertRGD motif for integrin binding fused to sfGFP and titin immunoglobulin domain (I27)
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFAB3689
Plasmid#47834PurposeBIOFAB RFP reporter plasmid for measuring promoter 3 + BCD1 efficiency.DepositorInsertpromoter 3 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3893
Plasmid#47844PurposeBIOFAB RFP reporter plasmid for measuring promoter 13 + BCD1 efficiency.DepositorInsertpromoter 13 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3992
Plasmid#47823PurposeBIOFAB RFP reporter plasmid for measuring promoter 14 + BCD16 efficiency.DepositorInsertpromoter 14 and BCD16
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3725
Plasmid#47837PurposeBIOFAB RFP reporter plasmid for measuring promoter 6 + BCD1 efficiency.DepositorInsertpromoter 6 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB3869
Plasmid#47842PurposeBIOFAB RFP reporter plasmid for measuring promoter 11 + BCD1 efficiency.DepositorInsertpromoter 11 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB1935
Plasmid#47860PurposeBIOFAB reporter plasmid for measuring his_T_min_linker_crp_T_min termination efficiencyDepositorInserthis_T_min_linker_crp_T_min double terminator
UseSynthetic BiologyTagsExpressionBacterialMutationconcatenate of his_min and crp_minPromoterpLTetO15Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Coh-ddFLN4-ELP-HIS-ybbr
Plasmid#153446PurposeE. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.DepositorInsertRc.CohE
UseTags3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only