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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,938 results for: emb

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Showing: 1581 - 1600 of 11938 results

  2. opto-Rac1

    Plasmid
    #135396
    Purpose
    Expresses opto-Rac1 (BcLOV4-GGGSx2-WT_Rac1-GGGSx2-mCherry in mammalian cells in the pcDNA3.1 backbone.
    Depositor
    Brian Chow
    Publication
    Berlew et al Photochem Photobiol Sci. 2020 Mar 1;19(3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    opto-Rac1
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135396-plasmid-map-sequence-id-271132

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  3. pDG330

    Plasmid
    #100898
    Purpose
    SpCas9 with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100898-plasmid-map-sequence-id-195326

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  11. CIBN-GFP-TMD

    Plasmid
    #79571
    Purpose
    expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)
    Depositor
    Pietro De Camilli, Olof Idevall-Hagren
    Publication
    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    syntaxin 1A (Stx1a Mouse)
    Tags
    CIBN and EGFP
    Expression
    Mammalian
    Mutation
    aa 249_288 (C-terminal region (polybasic stretch …
    Promoter
    CMV
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    79571-plasmid-map-sequence-id-456519

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  19. SpyTag-RBD

    Plasmid
    #159999
    Purpose
    Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells
    Depositor
    Mark Howarth
    Publication
    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159999-plasmid-map-sequence-id-313235

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Showing: 1581 - 1600 of 11938 results