We narrowed to 14,202 results for: nts

1. P427-FBP_13

   Plasmid

   #139704

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   various

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19/mChe-Px

   Plasmid

   #183170

   Purpose

   Construct for transient expression of peroxisomes-targeted mCherry in plants.

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   mCherry-Px
   

   Use

   Tags

   10 aa peroxisome targeting signal 1 (IHHPRELSRL) …

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19/Pd-mChe

   Plasmid

   #183172

   Purpose

   Construct for transient expression of plasmodesmata-targeted mCherry in plants.

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   Pd-mCherry
   

   Use

   Tags

   AtPDCB1, A. thaliana PLASMODESMATA CALLOSE-BINDIN…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19/mChe-Cs

   Plasmid

   #183171

   Purpose

   Construct for transient expression of cytoskeleton-targeted mCherry in plants.

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   mCherry-Cs
   

   Use

   Tags

   363 aa of A. thaliana FIMBRIN 1 (AtFIM1)

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19/CF-mChe

   Plasmid

   #183166

   Purpose

   Construct for transient expression of cytoplasmic foci-targeted mCherry in plants.

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   CF-mCherry
   

   Use

   Tags

   AtTZF1, A. thaliana TANDEM ZINC FINGER PROTEIN 1

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19/Tp-mChe

   Plasmid

   #183165

   Purpose

   Construct for transient expression of tonoplast-targeted mCherry in plants.

   Depositor

   Qinlong Zhu, Yao-Guang Liu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   Tp-mCherry
   

   Use

   Tags

   28 aa of A. thaliana CALCINEURIN B-LIKE PROTEIN 2…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. puc19-pCas13a

   Plasmid

   #118963

   Purpose

   Intermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.

   Depositor

   Magdy Mahfouz

   Article

   Aman et al Genome Biol. 2018 Jan 4;19(1):1. doi: 10

   Insert

   Cas13a
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB602-RBCS-tagRFP

   Plasmid

   #213868

   Purpose

   Binary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)

   Depositor

   Yutaka Kodama

   Article

   Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

   Insert

   RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEmpty_Control

   Plasmid

   #162607

   Purpose

   Empty control for Cas9 experiments and to clone new guide RNAs for paper

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9 with RPL25 Intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUbi-MAD7

    Plasmid

    #170133

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al J Genet Genomics. 2021 May 3. pii: S1673

    Insert

    MAD7(ErCas12a)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    maize Ubiquitin-1

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJ151-HDR

    Plasmid

    #113631

    Purpose

    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

    Depositor

    Alex Kentsis

    Inserts

    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    

    Use

    Cre/Lox; Homology recombination selection vector

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a promoter, EF1a promoter (T2A cleaving sequen…

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3.0_nucLAMA-G97 IRES EGFP

    Plasmid

    #136620

    Purpose

    Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-G97-NLS (IRES EGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pH-A3A-PBE

    Plasmid

    #119774

    Purpose

    Conversion of C to T in rice plants transformation

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS-UGI-NLS
    

    Use

    Tags

    NLS (flanking UGI)

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

    Plasmid

    #115698

    Purpose

    ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae

    Depositor

    Eckhard Boles

    Article

    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

    Insert

    SSKar2-sfpHluorin-HDEL
    

    Use

    Tags

    ER retention signal containing HDEL and ER signal…

    Expression

    Yeast

    Mutation

    for introduced mutations see publication

    Promoter

    MET25

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3.0_nucNanobody IRES EGFP

    Plasmid

    #136619

    Purpose

    Plasmid encoding nuclear localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-EnhancerNb-NLS (IRES EGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPath-pTac-integrase

    Plasmid

    #199999

    Purpose

    Entry vector for biosynthetic pathway. pTac-phiC31 integrase. This material contains 2 plasmids. For more information look under the "Depositor Comments" section below.

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Pathways
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. P305-FBP_4

    Plasmid

    #139695

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET51b(+)_LAMA-F98 (for GFP)

    Plasmid

    #130714

    Purpose

    Plasmid encoding the bacterial expression of LAMA-F98 for GFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    LAMA-F98
    

    Use

    Tags

    Hisx10

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3.0_mitoLAMA-F98

    Plasmid

    #130704

    Purpose

    Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    NTOM20-SNAP-Tag-LAMA-F98
    

    Use

    Tags

    NTOM20 and SNAP-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET51b(+)_LAMA-G97 (for GFP)

    Plasmid

    #130715

    Purpose

    Plasmid encoding the bacterial expression of LAMA-G97 for GFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    LAMA-G97
    

    Use

    Tags

    Hisx10

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge