We narrowed to 2,547 results for: organization Addgene

1. pcDNA3.cytGAP

   Plasmid

   #78734

   Purpose

   Expression of Ca2+ sensor GAP in the cytosol

   Depositor

   Teresa Alonso

   Article

   Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

   Insert

   cytGAP
   

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. SP-GFP-CD274-UTR

   Plasmid

   #121142

   Purpose

   Mammalian expression of GFP tagged CD274 with its own 3'UTR

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   CD274 with its own 3'UTR (CD274 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV-PM

   Plasmid

   #197386

   Purpose

   To constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).

   Depositor

   Qiao Zhou

   Article

   Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

   Insert

   PDX1-T2A-MAFA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ZJOM153

   Plasmid

   #133642

   Purpose

   Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast vacuole.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   Vph1-2GFP (VPH1 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. ZJOM31

   Plasmid

   #133645

   Purpose

   Integration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-TGL3-GFP (TGL3 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. ZJOM144

   Plasmid

   #133630

   Purpose

   Integration of SS-GFP-HDEL in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae) Marker for yeast endoplasmic reticulum (ER).

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   pTPI1-SS-GFP-HDEL
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.nucGAP

   Plasmid

   #78736

   Purpose

   Expression of Ca2+ sensor GAP in the nucleus

   Depositor

   Teresa Alonso

   Article

   Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

   Insert

   nucGAP
   

   Tags

   nucleoplasmin (Xenopus)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pLSU4cg10

   Plasmid

   #126078

   Purpose

   Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Insert

   Chloroplastic FNR transit peptide
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Promoter

   longCaMV35S

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUF-AsCpf1-pre-gRNA

   Plasmid

   #137849

   Purpose

   AsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

   Depositor

   Josh Beck

   Article

   Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

   Insert

   AsCpf1 and pre-gRNA cassette
   

   Use

   CRISPR

   Available Since

   April 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUF-LbCpf1-pre-gRNA

    Plasmid

    #137848

    Purpose

    LbCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

    Depositor

    Josh Beck

    Article

    Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

    Insert

    LbCpf1-NLS-FLAG and gRNA cassette
    

    Use

    CRISPR

    Available Since

    April 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. NPC1-CFP P692S

    Plasmid

    #58457

    Purpose

    expression of NPC1-CFP sterol sensing domain mutant

    Depositor

    Matthew Scott

    Article

    Ko et al Mol Biol Cell. 2001 Mar;12(3):601-14.

    Insert

    NPC1 (Npc1 Mouse)
    

    Tags

    ECFP and His6

    Expression

    Mammalian

    Mutation

    P692S sterol sensing domain mutant

    Promoter

    CMV

    Available Since

    Oct. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pER-eZinCh-2

    Plasmid

    #70009

    Purpose

    expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

    Depositor

    Maarten Merkx

    Article

    Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

    Insert

    ER-eZinCh-2
    

    Tags

    KDEL sequence and PPI signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKM263-EcPPXc

    Plasmid

    #38328

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    E. coli polyphosphatase (ppx Escherichia coli)
    

    Tags

    6xHIS, GST, and TEV

    Expression

    Bacterial

    Mutation

    C-terminus of E. coli polyphosphatase (described …

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNS3

    Plasmid

    #131770

    Purpose

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    

    Use

    Integration into δ-sites

    Expression

    Yeast

    Mutation

    E490G to make Cry2 into Cry2Olig

    Promoter

    PADH1

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Keratin 8 S73A

    Plasmid

    #18056

    Depositor

    M Bishr Omary

    Article

    Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

    Insert

    Keratin 8 (KRT8 Human)
    

    Expression

    Mammalian

    Mutation

    S73A

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pNS1

    Plasmid

    #131769

    Purpose

    PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    FUSN_Fusion Red_Cry2WT
    

    Use

    Integration into δ-sites

    Expression

    Yeast

    Promoter

    PADH1

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. KG#371

    Plasmid

    #110882

    Purpose

    Expresses the lysosome marker CTNS-1-mCherry cDNA in a subset of ventral cord cholinergic motor neurons

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2013 May;194(1):143-61. doi: 1

    Inserts

    unc-129 promoter
    CTNS-1a-mCherry cDNA
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    

    Tags

    intron-mCherry

    Expression

    Bacterial

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Keratin 8 S73D

    Plasmid

    #18054

    Depositor

    M Bishr Omary

    Article

    Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

    Insert

    Keratin 8 (KRT8 Human)
    

    Expression

    Mammalian

    Mutation

    S73D

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pFA-sbtA

    Plasmid

    #133011

    Purpose

    pFA carrying a Synechococcus elongatus PCC 7942 sbtA gene (Uniprot: Q6UP27)

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Insert

    SbtA
    

    Tags

    6xhis

    Expression

    Bacterial

    Promoter

    tetR

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Keratin 8 S431A

    Plasmid

    #18058

    Depositor

    M Bishr Omary

    Article

    Ku et al J Biol Chem. 2002 Mar 29. 277(13):10775-

    Insert

    Keratin 8 (KRT8 Human)
    

    Expression

    Mammalian

    Mutation

    S431A

    Available Since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only