175,750 results
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Plasmid#78953PurposeFigure 2. Expresses BxbI.DepositorInsertBxbI
Available SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-16b-10His-TEV-MCES_VACCA_D1_D12
Plasmid#203671PurposeThe D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavageDepositorInsertMCES_VACCA_D1_D12
Tags10xHisExpressionBacterialAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
rEstus
Plasmid#209767PurposeTo express a bright and sensitive genetically encoded voltage indicator in mammalian cells.DepositorInsertrEstus
ExpressionMammalianPromoterCMVAvailable SinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-puro-CMV-VC3AI
Plasmid#78907PurposeIndicator of caspase-3-like protease activityDepositorInsertVC3AI
UseLentiviralTagsHA and MycMutationS320A, deletion of AA1-200PromoterCMVAvailable SinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCCL-CellCycle
Plasmid#132429PurposeExpressing Fucci cell cycle indicator in mammalian cellsDepositorInserthCdt, hGeminin
UseLentiviralExpressionMammalianAvailable SinceOct. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Villinpromoter-blue-FlpOERT2
Plasmid#67278Purpose4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertVillin-mTQ2-P2A-FlpO-ERT2
TagsmTQ2, linked to FlpO through an P2A ribosomal ski…ExpressionMammalianPromoterVillinAvailable SinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (AAV5)
Viral Prep#100835-AAV5PurposeReady-to-use AAV5 particles produced from pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (#100835). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
iSpyMac
Plasmid#155014PurposeExpresses the codon-optimized, engineered iSpyMac Cas9 nuclease in mammalian cellsDepositorInsertiSpyMac
ExpressionMammalianMutationPID grafted onto SpyCas9 N-terminal domain, R221K…PromoterEF1ɑAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX459-sgAAVS1
Plasmid#184403PurposepX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGATDepositorInsertAAVS1-specific sgRNA
UseCRISPRExpressionMammalianPromoterU6 promoterAvailable SinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 (AAV1)
Viral Prep#100849-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 (#100849). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent NES-jRCaMP1b calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only