We narrowed to 32,487 results for: Eng;

1. pBabe-PTK6-YF

   Plasmid

   #210715

   Purpose

   Construct for stable expression of constitutively active human PTK6 in mammalian cells.

   Depositor

   Angela Tyner

   Article

   Zheng et al Mol Cell Biol. 2010 Sep;30(17):4280-92.

   Insert

   Protein tyrosine kinase 6 (PTK6 Human)
   

   Expression

   Mammalian

   Mutation

   Tyrosine 447 changed to Phenylalanine

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128347

   Purpose

   pAAV encoding control gRNA for CRISPR gRNAs listed above

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   control (negative)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hsyn-NE2h

   Plasmid

   #208687

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neurons

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

   Has Service

   AAV5

   Insert

   GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hsyn-NE2m

   Plasmid

   #208686

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m in neurons

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

   Insert

   GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG dCas9-KRAB-2A-EGFP

   Plasmid

   #92396

   Purpose

   CAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   dCas9_KRAB_2A_EGFP
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisplay-NE2m-IRES-mCherry-CAAX

   Plasmid

   #208690

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

   Insert

   GPCR activation based norepinephrine (NE) sensor GRAB_NE2m
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. SuExp-hPLD4

   Plasmid

   #201244

   Purpose

   express full length human PLD4 protein

   Depositor

   David Nemazee

   Article

   Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

   Insert

   PLD4 (PLD4 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDisplay-NE2h-IRES-mCherry-CAAX

   Plasmid

   #208691

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cells

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

   Insert

   GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI H2B-RFP

   Plasmid

   #92398

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   IRES H2B RFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-CFP-SaCas9-HF (without sgRNA)

    Plasmid

    #134470

    Purpose

    Human expression vector for SaCas9-HF variant.

    Depositor

    Zongli Zheng

    Article

    Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

    Insert

    Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
    

    Use

    CRISPR

    Tags

    SV40NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    R245A, N413A, N419A, R654A

    Promoter

    CAG

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFB-U6-sgNeuN-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128346

    Purpose

    pAAV encoding control gRNA for CRISPR gRNAs listed above

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Rbfox3 (NeuN) (Rbfox3 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330a dCas9-LSD1

    Plasmid

    #92362

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-LSD1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX458-DsRed2

    Plasmid

    #112219

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    DsRed2
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    DsRed2 contains a silent nucleotide substitution …

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330a dCas9-KRAB

    Plasmid

    #92361

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-KRAB
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pdRBFC-NS1-YN

    Plasmid

    #74723

    Purpose

    Express C-terminal YN fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labeling

    Depositor

    Aiming Wang

    Article

    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

    Insert

    Influenza A virus NS1 protein, partial
    

    Use

    Double-stranded rna labeling

    Tags

    HA and YFP N-terminal half domain

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcU6_3 sgRNA

    Plasmid

    #92359

    Purpose

    Chicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    chick U6.3 promoter and gRNA cloning cassette
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    chick U6.3

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pdRBFC-NS1-YC

    Plasmid

    #74724

    Purpose

    Express C-terminal YC fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labeling

    Depositor

    Article

    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

    Insert

    Influenza A virus NS1 protein, partial
    

    Use

    Double-stranded rna labeling

    Tags

    FLAG and YFP C-terminal half domain

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pdRBFC-B2-YN

    Plasmid

    #74721

    Purpose

    Express C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling

    Depositor

    Aiming Wang

    Article

    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

    Insert

    Influenza A virus NS1 protein, partial
    

    Use

    Double-stranded rna labeling

    Tags

    HA and YFP N-terminal half domain

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pdRBFC-B2-YC

    Plasmid

    #74722

    Purpose

    Express C-terminal YC fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labeling

    Depositor

    Aiming Wang

    Article

    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1

    Insert

    Influenza A virus NS1 protein, partial
    

    Use

    Double-stranded rna labeling

    Tags

    FLAG and YFP C-terminal half domain

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    May 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCI H2B-GFP

    Plasmid

    #92399

    Purpose

    Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-EGFP reporter.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    IRES H2B EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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