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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,917 results for: FRT

Showing: 1601 - 1620 of 1917 results

  3. pKK-mTagBFP-TEV

    Plasmid
    #105772
    Purpose
    Expression of your protein of interest in fusion with blue fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mTagBFP-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105772-plasmid-map-sequence-id-203361

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  5. pcDNA5-LAP-KNL1-M3

    Plasmid
    #115896
    Purpose
    Used for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.
    Depositor
    Geert Kops
    Article
    Vleugel et al J Cell Biol. 2013 Dec 23;203(6):943-55.
    Insert
    KNL1-M3 (KNL1 Human)
    Use
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Fragment of KNL1 containing three KNL1 repeat seq…
    Promoter
    Available Since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    115896-plasmid-map-sequence-id-224847

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  9. pKK-TEV-mEGFP

    Plasmid
    #105794
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105794-plasmid-map-sequence-id-211381

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  11. pKK-TEV-mTagBFP

    Plasmid
    #105789
    Purpose
    Expression of your protein of interest in fusion with blue fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 18940671, 25378625). Useful for multi-channel live cell imaging.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mTagBFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105789-plasmid-map-sequence-id-203380

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  13. pRD439

    Plasmid
    #168465
    Purpose
    For the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS-mTurquoise2-H-NSdbd-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available Since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168465-plasmid-map-sequence-id-331915

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  14. pSF3-Flag-Cbir-Cdc25C S216A_Myc-Nbir-14-3-3e

    Plasmid
    #90011
    Purpose
    Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Cdc25C S216A and N-terminally tagged NBir[2-256]-14-3-3e
    Depositor
    Julien Béthune
    Article
    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.
    Inserts
    CBirA*-Cdc25C S216A
    NBirA*-14-3-3e
    Use
    Retroviral; Flp/frt
    Tags
    FLAG and Myc
    Expression
    Mammalian
    Mutation
    Ser 216 to Ala
    Promoter
    CMV
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    90011-plasmid-map-sequence-id-201243

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  17. pKK-mVenus-TEV

    Plasmid
    #105774
    Purpose
    Expression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mVenus-TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105774-plasmid-map-sequence-id-203363

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  18. enDR3-TEV-mEGFP-NLS (pRS1451)

    Plasmid
    #240546
    Purpose
    Expression of the engineered N-terminal hybrid-binding domain of RNase H3 (enDR3) for capturing R-loops. A TEV cleavage site, mEGFP and nuclear localization signal (NLS) are included in the fusion.
    Depositor
    Roman Szczęsny
    Article
    Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk
    Insert
    enDR3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L52M
    Promoter
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240546-plasmid-map-sequence-id-481872

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  19. nb-enDR3-TEV-mEGFP-NLS (pRS1458)

    Plasmid
    #240547
    Purpose
    Expression of negative control for R-loops capture experiments using enDR3. A mutated, non-R-loops binding version of enDR3 is expressed in fusion with the TEV cleavage site, mEGFP, and NLS.
    Depositor
    Roman Szczęsny
    Article
    Jedynak-Slyvka et al Nucleic Acids Res. 2025 Aug 11;53(15):gk
    Insert
    nb-enDR3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S48A, K50A
    Promoter
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240547-plasmid-map-sequence-id-481875

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  20. pEXP(FLAG/HA-NSP1-K164-H165A)

    Plasmid
    #188782
    Purpose
    Expresses FLAG/HA-NSP1-K164-H165A in mammalian cells (SARS-CoV-2 mutant NSP1)
    Depositor
    Markus Hafner
    Article
    Anastasakis et al PLoS One. 2024 Jan 26;19(1):e0297262. do
    Insert
    FLAG/HA-NSP1-K164-H165A (SARS-CoV-2)
    Use
    Frt site to insert into hek293 t-rex genome
    Tags
    FLAG/HA
    Expression
    Mammalian
    Mutation
    Changed Lysine 164 to Alanine and Histidine 165 t…
    Promoter
    CMV Dox inducible
    Available Since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    188782-plasmid-map-sequence-id-372761

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Showing: 1601 - 1620 of 1917 results