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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 7,041 results for: Ank

Showing: 4051 - 4100 of 7041 results

  5. INCTbiosyn-pEF-GFP(rc)Bxb1

    Plasmid
    #127511
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127511-plasmid-map-sequence-id-250763

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  9. INCTbiosyn-p35S-GFP(rc)phiC31

    Plasmid
    #127527
    Purpose
    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127527-plasmid-map-sequence-id-250842

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  10. INCTbiosyn-p35S-GFP(rc)Bxb1

    Plasmid
    #127528
    Purpose
    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127528-plasmid-map-sequence-id-250843

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  12. INCTbiosyn-pEF-GFP(rc)phiC31

    Plasmid
    #127510
    Purpose
    Plasmid has an EF1 alpha promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127510-plasmid-map-sequence-id-250757

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  14. pYPQ289-RVR

    Plasmid
    #138127
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a with D513R, K519V and N523R mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    ErCas12a RVR variant
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    D513R, K519V and N523R
    Promoter
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138127-plasmid-map-sequence-id-272088

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  15. pYPQ284-RVR

    Plasmid
    #138117
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V and N573R mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    Mb2Cas12a RVR variant
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    N563R, K569V and N573R
    Promoter
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138117-plasmid-map-sequence-id-272074

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  16. pCJ199

    Plasmid
    #162672
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162672-plasmid-map-sequence-id-321466

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  19. pCJ197

    Plasmid
    #162670
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    S131G; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162670-plasmid-map-sequence-id-321452

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  20. INCTbiosyn-p35S(rc)2_4_5-GFP

    Plasmid
    #127520
    Purpose
    Plasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.
    Depositor
    Martin Bonamino
    Article
    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:
    Insert
    CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    127520-plasmid-map-sequence-id-250826

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  24. pCJ203

    Plasmid
    #162678
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaF2-A75; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162678-plasmid-map-sequence-id-321524

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  27. pB-TR-TTHA

    Plasmid
    #232479
    Purpose
    Tetracycline inducible PiggyBac vector expressing bacterial TTHA1718 (TTHA) gene gene including flag-tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Article
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Thermus thermophilus HB8
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-TetO
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232479-plasmid-map-sequence-id-471676

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  36. pET-hMICU1EF

    Plasmid
    #199180
    Purpose
    This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.
    Depositor
    Ming-Feng Tsai
    Article
    Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12
    Insert
    MICU1 (MICU1 Human)
    Use
    Tags
    His6 and MBP, Thrombin site, TEV site
    Expression
    Mutation
    D421A, E432K, F433W, F453W
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    199180-plasmid-map-sequence-id-403144

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Showing: 4051 - 4100 of 7041 results