We narrowed to 2,475 results for: T2A

1. AV0.1-CWS-Cre-mCherry

   Plasmid

   #192877

   Purpose

   coexpression of Cre and mCherry

   Depositor

   Kazuhiro Oka

   Insert

   Cre-T2A-mCherry
   

   Use

   AAV

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. STEME-4

   Plasmid

   #138140

   Purpose

   Targeted simultaneous C-to-T and A-to-G in rice protoplasts

   Depositor

   Caixia Gao

   Insert

   wtTadA-TadA7.10-APOBEC3A-nCas9-2X(T2A-UGI-NLS)
   

   Expression

   Plant

   Available Since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. STEME-3

   Plasmid

   #138139

   Purpose

   Targeted simultaneous C-to-T and A-to-G in rice protoplasts

   Depositor

   Caixia Gao

   Insert

   APOBEC3A-wtTadA-TadA7.10-nCas9-2X(T2A-UGI-NLS)
   

   Expression

   Plant

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-CMV5-N-cG-P

   Plasmid

   #192883

   Purpose

   coexpression of Nanoluc, copGFP and puromycin resistant marker

   Depositor

   Kazuhiro Oka

   Insert

   Nanoluc-P2A-copGFP-T2A-Puro
   

   Use

   Lentiviral

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti-EF1a-dCas9-KRAB-Puro

   Plasmid

   #99372

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-KRAB-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TO-hNIL BSD-mApple

   Plasmid

   #182313

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   hNGN2 (NEUROG2 Human)
   hISL1 (ISL1 Human)
   hLHX3 (LHX3 Human)
   bsd-T2A-mycNLS-mApple
   

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV hUbC-dCas9-MQ1(Q147L)-EGFP

   Plasmid

   #89793

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR and Lentiviral

   Tags

   3*FLAG and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Q147L, CAA-->CTA

   Promoter

   hUbC

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMaRSC

   Plasmid

   #89189

   Purpose

   The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.

   Depositor

   David Tirrell

   Article

   Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

   Insert

   Flag-L274G-T2A-mCherry (Mars1 Mouse)
   

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ10704

   Plasmid

   #183957

   Purpose

   U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   SKSKSO array
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-TagBFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-TetON-mEGFP-MYOD1_AroPERFECT

    Plasmid

    #215620

    Purpose

    For integration of MYOD1 AroPERFECT T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    MYOD1 AroPERFECT (MYOD1 Human)
    

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-EF1a-dCas9-VP64-Puro

    Plasmid

    #99371

    Purpose

    3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

    Depositor

    Kristen Brennand

    Article

    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

    Insert

    dCas9-VP64-T2A-Puro
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGF1-4eCOL2A1

    Plasmid

    #97210

    Purpose

    Lentiviral expression of copGFP-T2A-FLuc under control of a COL2A1-based, chondrogenesis-responsive promoter

    Depositor

    Ryan Porter

    Article

    De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

    Insert

    4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    COL2A1 regulatory elements

    Available Since

    Oct. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. ATG-2460

    Plasmid

    #108713

    Purpose

    EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)

    Depositor

    Keith Wood

    Article

    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

    Insert

    CBR2opt-copGFP fusion
    

    Use

    Lentiviral

    Tags

    CBR2opt fused to copGFP

    Mutation

    Promega used directed evolution to create CBR (Cl…

    Promoter

    elongation factor-1

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pY094

    Plasmid

    #84743

    Purpose

    Expresses huAsCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huAsCpf1
    GFP
    

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-hA3A-eBE-Y130F

    Plasmid

    #113423

    Purpose

    Expresses hA3A-eBE-Y130F in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-eBE-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_Y130F

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMIA10.7

    Plasmid

    #214451

    Purpose

    BxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.

    Depositor

    Roger Foo

    Article

    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

    Inserts

    Tet-On 3G
    PuroR
    TRE3G CopGFP
    

    Use

    Bxbi donor

    Tags

    PuroR, T2A, and Tet-On 3G

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCKC775

    Plasmid

    #226626

    Purpose

    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, sfGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    7xTCF-LEF and EF1a

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCKC764

    Plasmid

    #226625

    Purpose

    piggyBAC pCAG-hCas9-T2A-Unbiased3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Chicken, Human, S. pyogenes)
    EF1a promoter, blasticidin resistance
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV and EF1a

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCKC776

    Plasmid

    #226627

    Purpose

    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, mCherry
    

    Use

    Synthetic Biology

    Tags

    oxygen dependent degron (ODD)

    Expression

    Mammalian

    Promoter

    4xHRE_YB TATA and EF1a

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGMKOSiE

    Plasmid

    #20865

    Depositor

    Keisuke Kaji

    Article

    Kaji et al Nature. 2009 Apr 9;458(7239):771-5. doi:

    Insert

    myelocytomatosis oncogene-F2A-Kruppel-like factor 4-T2A-POU domain, class 5, transcription factor 1-E2A-SRY-box containing gene (Myc Mouse)
    

    Expression

    Mammalian

    Available Since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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