We narrowed to 6,952 results for: crispr cas9 plasmids
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NM-01
Plasmid#69799PurposeBacterial expression of Cas9 with His, MBP, and Strep tagsDepositorInsertCas9
UseTagsHIS, MBP, and StrepIIExpressionBacterialMutationAspartate 10 to Alanine (D10A) and Histidine 840 …PromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMLS268
Plasmid#73726PurposeSapTrap SL2 C-terminal connector donor plasmidDepositorInsertSL2 (gdp-2/gdp-3 intergenic region)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMLS297
Plasmid#73737PurposeSapTrap STOP codon + let-858 UTR N-terminal connector donor plasmidDepositorInsertSTOP codon + let-858 3'-UTR
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMLS269
Plasmid#73727PurposeSapTrap SL2::SV40-NLS C-terminal connector donor plasmidDepositorInsertSL2 + SV40 NLS (gdp-2/gdp-3 intergenic region)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKEE401
Plasmid#91715PurposeCRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Kan resistanceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXEE401B
Plasmid#91716PurposeCRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistanceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
BPK2660
Plasmid#70709PurposeHuman expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK02
Plasmid#105133PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM126
Plasmid#105134PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCJ1021
Plasmid#228761PurposePlasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.DepositorInsertIft88 gRNAs (Ift88 Mouse)
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterhU6Available sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCJ1022
Plasmid#228762PurposePlasmid expressing Cas9 and 2 gRNAs for mouse Pkd2. Use for disruption of mouse Pkd2 in cultured cells.DepositorInsertPkd2 gRNAs (Pkd2 Mouse)
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterhU6Available sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAT11
Plasmid#204290PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT75
Plasmid#204306PurposeFlippase under heat shock control. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthpartsDepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT62
Plasmid#204301PurposeExpress phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.DepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT56
Plasmid#204300PurposeExpress phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.DepositorInsert3XP3-EGFP
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT43
Plasmid#204299PurposeFor introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.DepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPK764
Plasmid#65767PurposeBacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNADepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
UseCRISPRTags3x FLAG and NLSExpressionBacterialMutationPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX458-ECFP
Plasmid#112220PurposeCloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFPDepositorInsertECFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only
