We narrowed to 5,078 results for: mcs

1. 3495 pcDNA3 Bid p15

   Plasmid

   #8780

   Depositor

   Stanley Korsmeyer

   Article

   Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

   Insert

   Bid p15 (Bid Mouse)
   

   Expression

   Mammalian

   Available Since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

2. S12.2_Bridge + Target

   Plasmid

   #127671

   Purpose

   Ampicillin resistant multiplexing backbone with CRY2HA and mCherry

   Depositor

   Jennifer Phillips-Cremins

   Article

   Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLLX13

   Plasmid

   #79825

   Purpose

   Yeast and Bacterial shuttle vector, includes origin of transfer for conjugation, used for making a DNA capture vector

   Depositor

   Cammie Lesser, Stephen Lory

   Article

   Wolfgang et al Proc Natl Acad Sci U S A. 2003 Jul 8;100

   Type

   Empty backbone

   Use

   Yeast and bacteria shuttle vector, contains origi…

   Available Since

   Sept. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-Nvec-codonopti-Cas9-2xNLS

   Plasmid

   #141108

   Purpose

   Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis

   Depositor

   Matthew Gibson

   Article

   Hill et al Methods Mol Biol. 2022;2450:437-465. doi

   Insert

   Nematostella codon optimized Cas9
   

   Use

   Unspecified

   Mutation

   All amino acids coded for by codons optimized for…

   Promoter

   T7

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI-TPI_PTC160-xrRNA-4H

   Plasmid

   #108369

   Purpose

   Expresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
   

   Expression

   Mammalian

   Mutation

   PTC160

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-TPI_WT-xrRNA-4H

   Plasmid

   #108368

   Purpose

   Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   Wild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag Snail 8SA

   Plasmid

   #16222

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

   Insert

   Snail (SNAI1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   8SA: S97A, S101A, S105A, S108A, S112A, S116A, S12…

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pENTR Age HsSAS-6 ST

   Plasmid

   #46382

   Purpose

   For expression of N-terminal fusions.

   Depositor

   Pierre Gonczy

   Article

   Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

   Insert

   SAS-6 (SASS6 Human)
   

   Use

   Entry vector

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P1-merlin 1-339

   Plasmid

   #168128

   Purpose

   Bacterial expression of human Nf2/merlin 1-339

   Depositor

   Tina Izard

   Article

   Primi et al Matrix Biol Plus. 2021 Jul 1;12:100074.

   Insert

   Merlin (NF2 Human)
   

   Tags

   Glutathione S-transferase (GST)

   Expression

   Bacterial

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. HGXPRT

    Plasmid

    #64201

    Purpose

    Expression of Plasmodium falciparum HGXPRT

    Depositor

    Vern Schramm

    Article

    Hazleton et al Chem Biol. 2012 Jun 22;19(6):721-30. doi

    Insert

    Hypoxanthine guanine xanthine phosphoribosyltransferase
    

    Tags

    Histidine tag

    Expression

    Bacterial

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. Empty Anchor Control

    Plasmid

    #127665

    Purpose

    Encodes for puromycin resistance expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    Puro
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Pmyo3::tag:sl1(G16C)

    Plasmid

    #79003

    Purpose

    Expression of exogenous SL1 in C. elegans muscle

    Depositor

    Xiao Liu, Ge Zhan

    Article

    Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

    Insert

    sls-1.2 (sls-1.2 Nematode)
    

    Tags

    illumina-adapter

    Expression

    Worm

    Mutation

    G16C

    Promoter

    myo-3

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP Snail 8SA

    Plasmid

    #16229

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    8SA: S97A, S101A, S105A, S108A, S112A, S116A, S12…

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pMM2-hPMCA2x/b

    Plasmid

    #47582

    Depositor

    Emanuel Strehler

    Article

    Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

    Insert

    PMCA2x/b (ATP2B2 Human)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA_GFP_mTLN1-FL-LBD

    Plasmid

    #164839

    Purpose

    Expression of GFP tagged FL mouse talin1 mutant

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

    Insert

    TLN1 (Tln1 Mouse)
    

    Tags

    His8-GFP

    Expression

    Mammalian

    Mutation

    Lipid binding deficient mutations

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T MDM2 S186D

    Plasmid

    #16239

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Ser186 replaced by Asp

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

17. p5B3-T

    Plasmid

    #92021

    Purpose

    Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lysA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V

    Available Since

    June 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSEM389 - mosTI unc-119 - NNN - mScarlet(nls) - tbb-2

    Plasmid

    #182349

    Purpose

    mScarlet transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-4T MDM2 S166D

    Plasmid

    #16238

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Ser166 replaced by Asp

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pCMV Aquamarine-shadowY tandem

    Plasmid

    #157775

    Purpose

    Produces a fusion between Aquamarine and shadowY. It can be used as a positive control for FRET (using FLIM)

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Type

    Empty backbone

    Tags

    Aquamarine and shadowY

    Expression

    Mammalian

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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