We narrowed to 18,708 results for: met

1. rag2:myr-Akt2_pISceI

   Plasmid

   #231495

   Purpose

   Expresses myristolated Akt2 in zebrafish lymphoid and mesenchymal cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   Akt2 (Akt2 Mouse)
   

   Use

   Zebrafish expression

   Tags

   N-terminal myristolation signal

   Expression

   Mutation

   Promoter

   Available Since

   June 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

   Plasmid

   #182652

   Purpose

   Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   4xU6-tRNAM15
   

   Use

   Tags

   nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   Y306A/Y384F (AF) double mutant of Methanosarcina …

   Promoter

   CMV and U6

   Available Since

   May 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGMC00005

   Plasmid

   #166722

   Purpose

   sgRNA against human ASNS

   Depositor

   Raj Chari

   Article

   Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

   Insert

   ASNS (ASNS Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGMC00006

   Plasmid

   #166723

   Purpose

   sgRNA against human ASNS

   Depositor

   Raj Chari

   Article

   Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

   Insert

   ASNS (ASNS Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. EFS-SpdCas9-Dnmt3A/3L-WT

   Plasmid

   #222497

   Purpose

   Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Insert

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   EFS

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. LCV2_mCherry_AAVS1_sgRNA_2

   Plasmid

   #155105

   Purpose

   lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-3XHA-p53 Δ1-42

   Plasmid

   #196269

   Purpose

   Mammalian expression of 3xHA tagged p53 Δ1-42 (ΔTAD1)

   Depositor

   Laura Attardi

   Article

   Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

   Insert

   p53 Δ1-42 (Trp53 Mouse)
   

   Use

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   deleted amino acids 1 to 42

   Promoter

   CMV

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCKO_AAVS1_sgRNA_2

   Plasmid

   #155085

   Purpose

   lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX304_C12orf49-V5

   Plasmid

   #155110

   Purpose

   lentiviral plasmid for the exogneous expression of C-terminal V5-tagged C12orf49

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   C12orf49 (SPRING1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   CMV promoter

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

    Plasmid

    #71213

    Purpose

    dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A
    

    Use

    Tags

    dSpCas9-BFP-DNMT3A(E752A)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. LCV2_mClover3_AAVS1_sgRNA_2

    Plasmid

    #155099

    Purpose

    lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid

    #127668

    Purpose

    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and hU6

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNIA-CEN-mVenus-FLAG-LANS1-Set2

    Plasmid

    #122003

    Purpose

    mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

    Depositor

    Brian Kuhlman

    Article

    Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

    Insert

    mVenus-FLAG-LANS1-Set2 (SET2 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    mVenus-FLAG

    Expression

    Yeast

    Mutation

    Set2 point mutations K538G, K539S, R549G, K550S, …

    Promoter

    ADH1

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. LCV2_AAVS1_sgRNA_3

    Plasmid

    #155089

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.2-Cx43-M100/125/147/213-siResist

    Plasmid

    #49858

    Purpose

    Encodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNA

    Depositor

    Robin Shaw

    Article

    Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

    Insert

    Connexin 43 (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Methionine100, Methionine 125, Methionine 147, an…

    Promoter

    CMV

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

16. rag2:LRRC10_CE_pISceI

    Plasmid

    #223042

    Purpose

    Express LRRC10 gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    LRRC10 (LRRC10 Human)
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Tags

    Expression

    Mutation

    Promoter

    Rag2

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. rag2:BEST3_CE_pISceI

    Plasmid

    #223043

    Purpose

    Express BEST3 gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    BEST3 (BEST3 Human)
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Tags

    Expression

    Mutation

    Promoter

    Rag2

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. rag2:MARCH9_CE_pISceI

    Plasmid

    #223027

    Purpose

    Express MARCH--9 gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    MARCH--9 (MARCH9 Human)
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Tags

    Expression

    Mutation

    Promoter

    Rag2

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. rag2:MDM2_CE_pISceI

    Plasmid

    #223035

    Purpose

    Express MDM2 gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Tags

    Expression

    Mutation

    Promoter

    Rag2

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. rag2:CPM_CE_pISceI

    Plasmid

    #223036

    Purpose

    Express CPM gene in mesenchymal lineage of Zebrafish.

    Depositor

    Alejandro Gutierrez

    Article

    Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

    Insert

    CPM (CPM Human)
    

    Use

    Express the insert(gene) in mesenchymal lineage o…

    Tags

    Expression

    Mutation

    Promoter

    Rag2

    Available Since

    Feb. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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