We narrowed to 12,487 results for: sas

1. 1352wUV2 F3bbs kan NNNTGGGCGk

   Plasmid

   #18756

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   3 tandem zinc fingers
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A Kan cassette (1090bp) is inserted between two b…

   Promoter

   Available since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 1352wUV2 F1bbs kan GCGTGGNNN

   Plasmid

   #18753

   Depositor

   Scot Wolfe

   Article

   Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

   Insert

   3 tandem zinc fingers
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A Kan cassette (1090bp) is inserted between two b…

   Promoter

   Available since

   July 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCfB2989

   Plasmid

   #63636

   Purpose

   pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

   Plasmid

   #69227

   Purpose

   Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TS4 ZFP
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcFLAG-Grap2-WT

   Plasmid

   #74511

   Purpose

   Overexpression of Grap2 in mammalian cells

   Depositor

   Lars Rönnstrand

   Article

   Chougule et al Oncotarget. 2016 Feb 15. doi: 10.18632/o

   Insert

   Grap2 (GRAP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pCfB2802

   Plasmid

   #63655

   Purpose

   pCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pResQ shDna2'

   Plasmid

   #31952

   Depositor

   Sheila Stewart

   Article

   Duxin et al Mol Cell Biol. 2009 Aug;29(15):4274-82.

   Insert

   shRNA against hDna2 (DNA2 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pSB1A3 EcoRI Methylase-AmilCP

   Plasmid

   #85166

   Purpose

   For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.

   Depositor

   Robin Dowell

   Article

   Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

   Insert

   EcoRI Methylase-AmilCP
   

   Use

   Tags

   AmilCP

   Expression

   Bacterial

   Mutation

   Promoter

   J23100 promoter

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pCfB2990

   Plasmid

   #63645

   Purpose

   pCfB2990 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   none

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2

    Plasmid

    #69225

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS2 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mEos2-FilaminA-C-14

    Plasmid

    #57378

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    FilaminA (FLNA Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    D2626H

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. EC71139

    Plasmid

    #154062

    Purpose

    Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Barley Construct)

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    pZmUbi
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    All BsaI, Esp3I and BpiI restriction sites were r…

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SauCas9KKH CCR5-nicking sgRNA

    Plasmid

    #169863

    Purpose

    SauCas9KKH nicking sgRNA for CCR5

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SauCas9KKH CCR5-nicking sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hC73A-pEGFP_N1

    Plasmid

    #111533

    Purpose

    Expresses human KCNB1 C73A in mammalian cells for imaging

    Depositor

    Federico Sesti

    Article

    Yu et al Cell Death Dis. 2017 Apr 6;8(4):e2737. d

    Insert

    human C73A-Kv2.1 (KCNB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C73A

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCLC3sFlag

    Plasmid

    #52422

    Purpose

    mammalian expression of flag tagged rat CLC3 short transcript

    Depositor

    Steven Weinman

    Article

    Shimada et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    CLC3 (Clcn3 Rat)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCfB2800

    Plasmid

    #63657

    Purpose

    pCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Insert

    GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3

    Plasmid

    #69226

    Purpose

    Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    TS3 ZFP
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pML2(−)WF

    Plasmid

    #108337

    Purpose

    MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

    Depositor

    Andrew Muscroft-Taylor

    Article

    van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCSDest2-SpCas9-MT3-NLS-3XHA-NLS-Zif268

    Plasmid

    #69224

    Purpose

    Expresses R1335K mutant SpCas9 fused to Zif268 in mammalian cell

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    NLS-3XHA-NLS and Zif268

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Promoter

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSMP-MBD1_1

    Plasmid

    #36365

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    MBD1 (MBD1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only