We narrowed to 2,612 results for: ubiquitin

1. pcDNA3-Flag-RNF168 delta RING

   Plasmid

   #133982

   Purpose

   mammalian expression vector of Flag tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deletion of RING

   Promoter

   CMV

   Available since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pEN-L1-K2-L2

   Plasmid

   #80684

   Purpose

   Gateway Entry vector containing a temperature-sensitive mouse DHFR

   Depositor

   Nico Dissmeyer

   Article

   Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

   Inserts

   DHFR (Dhfr Mouse)
   DHFR (Dhfr Mustard Weed, Mouse)
   

   Use

   Synthetic Biology

   Tags

   3xHA

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   mouse DHFR with N-terminal Ubiquitin

   Promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

   Plasmid

   #169168

   Purpose

   Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.

   Depositor

   Sascha Martens

   Article

   Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   MAP1LC3B (MAP1LC3B Human)
   

   Use

   Tags

   6X Histidine Tag, TEV cleavage site, mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7 lac promoter

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. mcry1K31R HA tag

   Plasmid

   #110297

   Purpose

   mammalian expression of full length mouse CRY1 mutant K31R

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

   Insert

   mcry1 K31R (Cry1 Mouse)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   31 Lysines are changed to Argenine

   Promoter

   CMV

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-eGFP-NLS-RNF168 delta RING

   Plasmid

   #133983

   Purpose

   mammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deletion of RING; siRNA resistant sequence: GAGGA…

   Promoter

   CMV

   Available since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pOPINK-ALG13 (OTU, aa 216-353)

   Plasmid

   #61414

   Purpose

   Expresses human ALG13 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-215 and aa 354-1137.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac1-NEDD4L_W2-W3-W4-HECT (349)

   Plasmid

   #187721

   Purpose

   Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 349-975 (NEDD4L Human)
   

   Use

   Tags

   GST-HRV 3C site

   Expression

   Insect

   Mutation

   deletion of residues 1-348

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT

   Plasmid

   #187723

   Purpose

   Express His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 1-418+582-975 (NEDD4L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   deletion of residues 419-581

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastBacHTA-NEDD4L_W1-L-W2-HECT

   Plasmid

   #187724

   Purpose

   Express His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L residues 153-418+582-975 (NEDD4L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   deletion of residues 1-152 and 419-581

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. His-Precission-NEDD4L (381-975)_pcDNA3.1(+)

    Plasmid

    #187725

    Purpose

    Express his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 381-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Mammalian

    Mutation

    deletion of residues 1-380

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. His-Precission-NEDD4L (492-975)_pcDNA3.1(+)

    Plasmid

    #187726

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT in mammalian cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Mammalian

    Mutation

    deletion of residues 1-491

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)

    Plasmid

    #187727

    Purpose

    Express his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L with residues 226-382 removed (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Mammalian

    Mutation

    deletion of residues 226-382

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)

    Plasmid

    #187728

    Purpose

    Express his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L with residues 418-495 removed (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Mammalian

    Mutation

    deletion of residues 418-495

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

    Plasmid

    #187708

    Purpose

    Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 381-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-380

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a(+)-NEDD4L_W3-W4-HECT (492)

    Plasmid

    #187709

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-491

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a(+)-NEDD4L_W3-W4-HECT_C874only

    Plasmid

    #187711

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 with mutations (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-491; mutations C702A, C776…

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX6P1-NEDD4L_FL-CS

    Plasmid

    #187713

    Purpose

    Express the catalytically inactive GST-tagged NEDD4L FL in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    Cys874 mutated to a serine

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX6P1-NEDD4L_Δ1,2-linker

    Plasmid

    #187714

    Purpose

    Express GST-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L with residues 226-382 removed (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 226-382

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX6P1-NEDD4L_Δ2,3-linker

    Plasmid

    #187715

    Purpose

    Express GST-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L with residues 418-495 removed (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 418-495

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX6P1-NEDD4L_W2-W3-W4-HECT (348)

    Plasmid

    #187716

    Purpose

    Express GST-tagged NEDD4L W2-W3-W4-HECT (348) in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L with residues XX (NEDD4L Human)
    

    Use

    Tags

    GST-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-347

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge