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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,640 results for: gan

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Showing: 1641 - 1660 of 14640 results

  2. mKate2-P2A-APEX2-ML1N*2

    Plasmid
    #67665
    Purpose
    Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain, [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.
    Depositor
    Rob Parton
    Publication
    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mKate2-P2A-APEX2-ML1N*2
    Expression
    Mammalian
    Available since
    Nov. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67665-plasmid-map-sequence-id-117213

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  5. ITGB1

    Plasmid
    #51920
    Purpose
    Expresses full-length Integrin beta-1 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ITGB1 (ITGB1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 728, before CD4, bio and…
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51920-plasmid-map-sequence-id-79898

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  9. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

    Plasmid
    #127353
    Purpose
    gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Publication
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TurboID (BirA mutant)
    Use
    Gateway Cloning
    Tags
    GS linker, NES, V5, and mVenus
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127353-plasmid-map-sequence-id-246311

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  12. pET21-Flavidin

    Plasmid
    #89881
    Purpose
    Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling
    Depositor
    Mark Howarth
    Publication
    Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-
    Insert
    Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
    Tags
    6 glutamate tag (C terminal on insert)
    Expression
    Bacterial
    Mutation
    K80R, K121R, K132R, K134R
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89881-plasmid-map-sequence-id-177281

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  14. pPD30_38

    Plasmid
    #1443
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    unc54enh, unc545', IVSA, rf2128
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1443-plasmid-map-sequence-id-54

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  18. SPARC-bio-His

    Plasmid
    #52078
    Purpose
    Expresses full-length Secreted protein acidic and rich in cysteine precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    SPARC (SPARC Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52078-plasmid-map-sequence-id-80303

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Showing: 1641 - 1660 of 14640 results