We narrowed to 3,311 results for: nif
-
Plasmid#165573PurposeDNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.DepositorInsertphi29 DNA Polymerase
UseSynthetic BiologyAvailable SinceAug. 24, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-MS2-HA-Hs4E-T_AA
Plasmid#148233PurposeMammalian Expression of Hs4E-TDepositorInsertHs4E-T (EIF4ENIF1 Human)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOpen-HSlig3
Plasmid#165574PurposeRepairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992))DepositorInsertDNA Ligase III
UseSynthetic BiologyAvailable SinceAug. 24, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
human EB1 in pBluescript II
Plasmid#39324DepositorAvailable SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTet_KaeCanABC(G104K,G189K)
Plasmid#248958PurposeCanABC from K. aerogenes under control of pTet and the native promoter, with ChyC mutated (G104K,G189K)DepositorInsertKaeCanABC(G104K,G189K)
ExpressionBacterialMutationG104K,G189KAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTet_PflHamMA(-cJun)B
Plasmid#248940PurposeHachiman from P. fluorescens under pTet control with cJun fused to the protease domain and natural linkerDepositorInsertflHamMA(-cJun)B
ExpressionBacterialMutationc-Jun insertion, HamA domain deletionAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLG017_FLAG-Avs1a(I134K,I234K)
Plasmid#248949PurposeAVAST from E. piriflorinigrans with an N-terminal 3xFLAG tag fused to Avs1a (I134K, I234K)DepositorInsertFLAG-Avs1a(I134K,I234K)
Tags3xFLAGExpressionBacterialAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTet_PflHamM(I118K,I218K)AB
Plasmid#248937PurposeHachiman from P. fluorescens under pTet control with HamM mutated (I118K,I218K)DepositorInsertPflHamM(I118K,I218K)AB
ExpressionBacterialMutationI118K,I218KAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only -
L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6
Plasmid#219712PurposeLevel 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.DepositorInsertGG4-pGATA23-Bxb1-tUBQ10-GG6
UseSynthetic BiologyAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)
Plasmid#219689PurposeLevel 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PsB (mtagBFP2, mCherry, NeonGreen)
ExpressionPlantAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4
Plasmid#219709PurposeLevel 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.DepositorInsertGG0-pSPCH:PhiC31:tUBQ1-GG4
UseSynthetic BiologyAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I84 - GG0-pAHP6-GG1
Plasmid#219706PurposeLevel 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.DepositorInsertGG0-pAHP6-GG1
UseSynthetic BiologyMutationWT promoter with added golden gate linkersAvailable SinceJune 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOpen-RNAse A
Plasmid#165565PurposeRibonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.DepositorInsertRNAse A
UseSynthetic BiologyAvailable SinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
human EB1 in pEGFP N1 to express EB1-GFP (JB131)
Plasmid#39299DepositorAvailable SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOpen-ETSSB
Plasmid#165549PurposessDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structureDepositorInsertExtreme Thermostable Single-Stranded DNA Binding Protein
UseSynthetic BiologyAvailable SinceAug. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
human EB1 fused to monomeric RFP
Plasmid#39323DepositorAvailable SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHMGWA-Pa14Csy4H29A
Plasmid#41092DepositorInsertCsy4
TagsHis6-MBPMutationHis29Ala mmutation to abolish ribonuclease activi…PromoterT7Available SinceDec. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCasY1
Plasmid#87687Purposep15A plasmid expressing CasY1DepositorInsertCasY1
ExpressionBacterialAvailable SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOpen-BsupolLF
Plasmid#165563PurposeBsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.DepositorInsertBsu DNA Polymerase I, Large Fragment
UseSynthetic BiologyAvailable SinceAug. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-Endonuclease IV E. coli
Plasmid#165551PurposeDNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups.DepositorInsertEndonuclease IV E. coli
UseSynthetic BiologyAvailable SinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits