We narrowed to 1,670 results for: nif

1. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE

   Plasmid

   #236239

   Purpose

   Lentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmerald

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   CaV2.1 (CACNA1A Rabbit)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Promoter

   human Synapsin 1

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV hSyn INTRON mEmerald-ER WPRE

   Plasmid

   #236230

   Purpose

   AAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulum

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)
   

   Use

   AAV

   Tags

   mEmerald

   Promoter

   human Synapsin 1

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV hSyn INTRON HaloTag-Sec61b WPRE

   Plasmid

   #236229

   Purpose

   AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   HaloTag-Sec61b (SEC61B Human, Synthetic)
   

   Use

   AAV

   Tags

   HaloTag

   Promoter

   human Synapsin 1

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

   Plasmid

   #236245

   Purpose

   Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
   

   Use

   AAV

   Tags

   mTagBFP2

   Promoter

   U6 and human Synapsin1

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE

   Plasmid

   #236240

   Purpose

   Lentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmerald

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   CaV2.2 (CACNA1B Rabbit)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Promoter

   human Synapsin 1

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV hSyn INTRON mEmerald-Sec61b WPRE

   Plasmid

   #236228

   Purpose

   AAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to mEmerald.

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mEmerald-Sec61b (SEC61B Human, Synthetic)
   

   Use

   AAV

   Tags

   mEmerald

   Promoter

   human Synapsin 1

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

   Plasmid

   #163701

   Purpose

   Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Inserts

   SOX2-3xHA-P2A-tagBFP (SOX2 Human)
   rtTA
   T2-sgRNA
   

   Use

   AAV and CRISPR

   Tags

   3x-HA and P2A-tagBFP

   Expression

   Mammalian

   Promoter

   CAG and TRE3G

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-CRY2oligo-mcherry-ANXA11

   Plasmid

   #164203

   Purpose

   Expresses CRY2oligo-mcherry tagged ANXA11 under CMV promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Tags

   CRY2oligo-mcherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX-EF1a ANXA11-mEmerald D40G

   Plasmid

   #164211

   Purpose

   pLEX lentivirus backbone expresses mEmerald tagged ANXA11 D40G mutant under EF1a promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

   Insert

   ANXA11 (ANXA11 Human)
   

   Use

   Lentiviral

   Tags

   mEmerald

   Mutation

   D40G

   Promoter

   EF1a

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-CRY2oligo-mCherry-ANXA11(186-505 aa)

    Plasmid

    #164208

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 C terminal under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    186-505 aa

    Promoter

    CMV

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-CRY2oligo-mcherry-ANXA11(1-185aa)

    Plasmid

    #164207

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 N terminal under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    1-185 aa

    Promoter

    CMV

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLEX-EF1a ANXA11-mEmerald R346C

    Plasmid

    #164213

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R346C mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Mutation

    R346C

    Promoter

    EF1a

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX-EF1a ANXA11-mEmerald R235Q

    Plasmid

    #164212

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 R235Q mutant under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Mutation

    R235Q

    Promoter

    EF1a

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET_His6-SUMO-TEV-TtCsm6

    Plasmid

    #172486

    Purpose

    Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

    Insert

    T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)
    

    Tags

    His-SUMO-TEV

    Expression

    Bacterial

    Promoter

    T7 promoter and lac operator

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMV-CRY2oligo-mcherry-ANXA11(R235Q)

    Plasmid

    #164205

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 R235R mutant under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    R235Q

    Promoter

    CMV

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CMV-CRY2oligo-mcherry-ANXA11(D40G)

    Plasmid

    #164204

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 D40G mutant under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    D40G

    Promoter

    CMV

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBFC1207

    Plasmid

    #186694

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    

    Expression

    Bacterial

    Promoter

    PLac

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRES2-smGFP-Kv2.1

    Plasmid

    #131709

    Purpose

    Expresses Kv2.1 in mammalian cells

    Depositor

    Jennifer Kearney

    Article

    Kang et al Ann Neurol. 2019 Dec;86(6):899-912. doi:

    Insert

    KCNB1 (KCNB1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-CRY2oligo-mcherry-ANXA11(R346C)

    Plasmid

    #164206

    Purpose

    Expresses CRY2oligo-mcherry tagged ANXA11 R346C mutant under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Tags

    CRY2oligo-mcherry

    Expression

    Mammalian

    Mutation

    R346C

    Promoter

    CMV

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV hSyn INTRON mScarlet-STIM2 WPRE

    Plasmid

    #236236

    Purpose

    AAV expression of human STIM2 internally tagged with mScarlet-I

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mScarletI-STIM2 (STIM2 Human)
    

    Use

    AAV

    Tags

    mScarletI in position 29

    Mutation

    Signal peptide from STIM1 and residues 15-746 of …

    Promoter

    human Synapsin 1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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