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We narrowed to 3,311 results for: nif

Showing: 1641 - 1660 of 3311 results
  1. pOpen-phi29pol

    Plasmid
    #165573
    Purpose
    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.
    Insert
    phi29 DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. pOpen-HSlig3

    Plasmid
    #165574
    Purpose
    Repairs single strand breaks in DNA efficiently. Unable to perform either blunt-end joining or AMP- dependent relaxation of supercoiled DNA (Elder, R.H. et al . , Bur. J. Biochem.. 203:53-58 (1992))
    Insert
    DNA Ligase III
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. pTet_KaeCanABC(G104K,G189K)

    Plasmid
    #248958
    Purpose
    CanABC from K. aerogenes under control of pTet and the native promoter, with ChyC mutated (G104K,G189K)
    Depositor
    Insert
    KaeCanABC(G104K,G189K)
    Expression
    Bacterial
    Mutation
    G104K,G189K
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
  4. pTet_PflHamMA(-cJun)B

    Plasmid
    #248940
    Purpose
    Hachiman from P. fluorescens under pTet control with cJun fused to the protease domain and natural linker
    Depositor
    Insert
    flHamMA(-cJun)B
    Expression
    Bacterial
    Mutation
    c-Jun insertion, HamA domain deletion
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
  5. pLG017_FLAG-Avs1a(I134K,I234K)

    Plasmid
    #248949
    Purpose
    AVAST from E. piriflorinigrans with an N-terminal 3xFLAG tag fused to Avs1a (I134K, I234K)
    Depositor
    Insert
    FLAG-Avs1a(I134K,I234K)
    Tags
    3xFLAG
    Expression
    Bacterial
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
  6. pTet_PflHamM(I118K,I218K)AB

    Plasmid
    #248937
    Purpose
    Hachiman from P. fluorescens under pTet control with HamM mutated (I118K,I218K)
    Depositor
    Insert
    PflHamM(I118K,I218K)AB
    Expression
    Bacterial
    Mutation
    I118K,I218K
    Available Since
    Feb. 23, 2026
    Availability
    Academic Institutions and Nonprofits only
  7. L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6

    Plasmid
    #219712
    Purpose
    Level 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites.
    Depositor
    Insert
    GG4-pGATA23-Bxb1-tUBQ10-GG6
    Use
    Synthetic Biology
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)

    Plasmid
    #219689
    Purpose
    Level 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Insert
    T2PsB (mtagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4

    Plasmid
    #219709
    Purpose
    Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
    Depositor
    Insert
    GG0-pSPCH:PhiC31:tUBQ1-GG4
    Use
    Synthetic Biology
    Available Since
    June 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. L0I84 - GG0-pAHP6-GG1

    Plasmid
    #219706
    Purpose
    Level 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.
    Depositor
    Insert
    GG0-pAHP6-GG1
    Use
    Synthetic Biology
    Mutation
    WT promoter with added golden gate linkers
    Available Since
    June 7, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. pOpen-RNAse A

    Plasmid
    #165565
    Purpose
    Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.
    Insert
    RNAse A
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. pOpen-ETSSB

    Plasmid
    #165549
    Purpose
    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure
    Insert
    Extreme Thermostable Single-Stranded DNA Binding Protein
    Use
    Synthetic Biology
    Available Since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  13. pOpen-BsupolLF

    Plasmid
    #165563
    Purpose
    Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.
    Insert
    Bsu DNA Polymerase I, Large Fragment
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  14. pOpen-Endonuclease IV E. coli

    Plasmid
    #165551
    Purpose
    DNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups.
    Insert
    Endonuclease IV E. coli
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1641 - 1660 of 3311 results