We narrowed to 3,150 results for: pam

1. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

   Plasmid

   #140002

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

   Plasmid

   #140004

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FRB_T2098L-VAMP3-mCherry

   Plasmid

   #92429

   Purpose

   Expression of VAMP3 N-terminally conjugated to FRB and C-terminally conjugated to mCitrine.

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

   Insert

   VAMP3 (Vamp3 Mouse)
   

   Use

   Tags

   FKBP rapamycin binding (FRB) domain of mTOR with …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET15p65

   Plasmid

   #27661

   Depositor

   Kathleen Collins

   Article

   Prathapam et al Nat Struct Mol Biol. 2005 Mar . 12(3):25

   Insert

   Tetrahymena telomerase holoenzyme p65
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCITEp65

   Plasmid

   #27659

   Depositor

   Kathleen Collins

   Article

   Prathapam et al Nat Struct Mol Biol. 2005 Mar . 12(3):25

   Insert

   Tetrahymena telomerase holoenzyme p65
   

   Use

   Reticulocyte lysate

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pIA030

   Plasmid

   #200424

   Purpose

   thrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

   Depositor

   Rut Carballido-López

   Article

   Altinoglu et al bioRxiv 2023

   Insert

   LacI
   

   Use

   Tags

   PAmCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Ppen

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTAHR TH-p2a-TD:Tomato (floxed selection Puro)

   Plasmid

   #135814

   Purpose

   Fluorescent reporter for knock-in of TdTomato into the TH locus

   Depositor

   Tim Ahfeldt, Lee Rubin

   Article

   Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S22

   Insert

   TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
   

   Use

   Tags

   p2a-TdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

   Plasmid

   #140003

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

   Plasmid

   #139997

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid

    #139996

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti.pFOXA2.GFP

    Plasmid

    #59404

    Purpose

    expresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

    Depositor

    Kai-Christian Sonntag

    Article

    Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

    Insert

    400 bp FOXA2 promoter, eGFP (FOXA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pFOXA2

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti.pFOXA2.5'.GFP

    Plasmid

    #59407

    Purpose

    expresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

    Depositor

    Kai-Christian Sonntag

    Article

    Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

    Insert

    approx. 1350 bp FOXA2 promoter, eGFP (FOXA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FOXA2

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RFP-HA-DAT

    Plasmid

    #90265

    Purpose

    Expresses RFP-HA-DAT in mammalian cells

    Depositor

    Alexander Sorkin

    Article

    Caltagarone et al Mol Cell Neurosci. 2015 Sep;68:120-30. d

    Insert

    DAT (dopamine transporter) (SLC6A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    inserted HA tag (see YFP-HA-DAT)

    Promoter

    CMV

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YFP-DAT

    Plasmid

    #90228

    Purpose

    Expresses human DAT in mammalian cells

    Depositor

    Alexander Sorkin

    Article

    Sorkina et al J Biol Chem. 2003 Jul 25;278(30):28274-8

    Insert

    DAT (dopamine transporter) (SLC6A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXD70EN25-Pct5-(–)-Eddh(Gp3C)

    Plasmid

    #233877

    Purpose

    Expresses Gordonibacter pamelaeae 3C (–)-Eddh in Eggerthella lenta DSM 2243 with a cumate inducible promoter

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    (–)-Eddh
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. YFP-HA-DAT

    Plasmid

    #90244

    Purpose

    Expresses YFP-HA-DAT in mammalian cells

    Depositor

    Alexander Sorkin

    Article

    Sorkina et al J Neurosci. 2006 Aug 2;26(31):8195-205.

    Insert

    DAT (dopamine transporter) (SLC6A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    residues 193–203 of human DAT were replaced in YF…

    Promoter

    CMV

    Available since

    June 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-EF1a-loxP-GRAB_DA2m-loxP

    Plasmid

    #189751

    Purpose

    Excludes sensor expression in Cre-positive neurons

    Depositor

    Yulong Li

    Article

    Dai et al Cell Rep. 2022 Aug 23;40(8):111246. doi:

    Insert

    Dopamine sensor GRAB_DA2m
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcihygroflagsynhDAT

    Plasmid

    #19990

    Depositor

    Jonathan Javitch

    Article

    Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97

    Insert

    Dopamine transporter (SLC6A3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-CBX1wt-PA-mCherry

    Plasmid

    #138252

    Purpose

    Wild-type CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry).

    Depositor

    Jin Zhang

    Article

    Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

    Insert

    CBX1-PAmCherry
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. YFP-DAT R60A

    Plasmid

    #90247

    Purpose

    Expresses YFP-DAT R60A mutant in mammalian cells

    Depositor

    Alexander Sorkin

    Article

    Ma et al Sci Rep. 2017 Jul 14;7(1):5399. doi: 10.

    Insert

    DAT (dopamine transporter) (SLC6A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutated R60 to A in human DAT cDNA

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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