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MXS_PGK::tTA2-bGHpA

Plasmid

#62448

Purpose

MXS_chaining vector with PGK::tTA2-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pYFAC

Plasmid

#168982

Purpose

Filamentous fungi vector. Also referred to as pYFAC-pyrG.

Depositor

Yit Heng Chooi

Article

Chooi et al Environ Microbiol. 2017 May;19(5):1975-1

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in fil…

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDS272

Plasmid

#34975

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2-GFP-LOVpep (MID2 Budding Yeast, A. sativa)

Expression

Yeast

Mutation

T406A, T407A

Promoter

TEF

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-WP1-CRY2PHR-VP64

Plasmid

#171758

Purpose

Lentiviral vector for constitutive expression of fusion protein WP1-CRY2PHR-VP64

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

WP1-CRY2PHR-VP64

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFPL-Cg

Plasmid

#61652

Purpose

Gateway vector for generating cyan fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced cyan fluorescent protein

Expression

Plant

Available Since

March 26, 2015

Availability

Academic Institutions and Nonprofits only
mEos2-Fibrillarin-7

Plasmid

#57377

Purpose

Localization: Nucleoli, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Fibrillarin (FBL Human)

Tags

mEos2

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pgRNA-guideless

Plasmid

#129464

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Guideless gRNA backbone

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pASKt15C+SrtA5

Plasmid

#64985

Purpose

Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, P94R, D160N, D165A, K19…

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-36α

Plasmid

#214343

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36A (IL36A Human)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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EmptyTranscriptionalReporter

Plasmid

#62453

Purpose

MXS_chaining vector with 2 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

CMVmin::NLS-Citrine-bGHpA
CMV::NLS-Cherry-bGHpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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