We narrowed to 11,264 results for: Vars

1. PX458-3xHA-en15FnCas9

   Plasmid

   #201948

   Purpose

   Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   3xHA-NLS-en15FnCas9-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   E1603H on FnCas9

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-SpCas9D10A nickase

   Plasmid

   #216737

   Purpose

   Expresses SpCas9-D10A nickase from a CMVd1 promoter. For AAV packaging. Derived from pAAV-CMV-SpCas9 (Addgene #113034)

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CMVd1

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. mRFP-FKBP12-5ptpase domain

   Plasmid

   #67516

   Purpose

   Recruitable human type IV 5-phosphatase domain (214-644)

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

   Insert

   FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)
   

   Use

   Tags

   mRFP

   Expression

   Mutation

   INPP5E has C641A to destroy the C-terminal CAAX d…

   Promoter

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pαH-S-GSAS-B.1.1.7

   Plasmid

   #171747

   Purpose

   Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-B.1.1.7 variant (UK)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Science. 2021 Jun 24. pii: science.abi62

   Insert

   Spike (S-GSAS-B.1.1.7 variant) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   July 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mRFP-FKBP12-5ptpase enzyme

   Plasmid

   #67515

   Purpose

   Recruitable human type IV 5-phosphatase enzyme

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

   Insert

   FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   INPP5E has C641A to destroy the C-terminal CAAX d…

   Promoter

   CMV

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLV(gRNA)-CMV-eGFP-U6(sgCTG)

   Plasmid

   #216732

   Purpose

   Contains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytes

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   sgCTG lentiviral guide RNA with eGFP tag
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVI

   Plasmid

   #176334

   Purpose

   Expresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-S2P-B.1.617.2 (S )
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   T19R, G142D, del156-157, R158G, L452R, T478K, D61…

   Promoter

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVRC8400-SARS-CoV-2-RBD-BA.2-AVI

   Plasmid

   #184533

   Purpose

   Expresses SARS-CoV-2 RBD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

   Insert

   SARS-CoV-2-RBD-BA.2 (S )
   

   Use

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Mutation

   G339D, S371F, S373P, S375F, T376A, D405N, R408S, …

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCB268

   Plasmid

   #53363

   Purpose

   pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.

   Depositor

   Alexander Varshavsky

   Article

   Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:

   Inserts

   DHFR (Dhfr Mouse)
   Ub
   Aβ42 (APP Human)
   

   Use

   Tags

   13xMyc, Flag, and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pVRC8400-SARS-CoV-2-S2P-B.1.1.7-AVI

    Plasmid

    #176325

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.1.7 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.1.7 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    69-70 del, 144del, N501Y, A570D, D614G, P681H, T7…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRL-MND-hCard11 R72L-T2A-GFP

    Plasmid

    #163784

    Purpose

    Expression of R72L CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R72L

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. R713-M50-303: CMV51p> Halotag-HRasHVR

    Plasmid

    #159685

    Purpose

    Mammalian protein expression of HRAS HVR (Hypervariable region)-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-HRasHVR (HRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. R713-M48-303: CMV51p> Halotag-Kras4aHVR

    Plasmid

    #159684

    Purpose

    Mammalian protein expression of KRAS4a HVR (Hypervariable region)-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Kras4aHVR (KRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. R703-M80-303: CMV51p> Halotag-Kras4bHVR

    Plasmid

    #159683

    Purpose

    Mammalian protein expression of KRAS4b HVR (Hypervariable region)-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-Kras4bHVR (KRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. R713-M52-303: CMV51p> Halotag-NRasHVR

    Plasmid

    #159686

    Purpose

    Mammalian protein expression of NRAS HVR (Hypervariable region)-Halotag fusion

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Insert

    Halotag-NRasHVR (NRAS Human)
    

    Use

    Tags

    Halotag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVRC8400-SARS-CoV-2-NTD-BA.2-AVI

    Plasmid

    #184532

    Purpose

    Expresses SARS-CoV-2 NTD domain from the BA.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-NTD-BA.2 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19I, L24-, P25-, P26-, A27S, G142D, V213G

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVRC8400-SARS-CoV-2-S2P-B.1.351-AVI

    Plasmid

    #176326

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.351 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.351 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L18F, D80A, D215G, 242-244del, R246I, K417N, E484…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVRC8400-SARS-CoV-2-NTD-P.1-AVI

    Plasmid

    #176429

    Purpose

    Expresses SARS-CoV-2 NTD domain from the P.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-NTD-P.1 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L18F, T20N, P26S, D138Y, R190S

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVRC8400-SARS-CoV-2-S2P-P.1-AVI

    Plasmid

    #176327

    Purpose

    Expresses SARS-CoV-2 S2P protein from the P.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-P.1 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pαH-S-GSAS-B.1.351

    Plasmid

    #171749

    Purpose

    Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-B.1.351 variant (South African)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Science. 2021 Jun 24. pii: science.abi62

    Insert

    Spike (S-GSAS-B.1.351 variant) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Promoter

    Available since

    July 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge