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We narrowed to 2,441 results for: dcas9

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Showing: 1661 - 1680 of 2441 results

  1. TRE3GV-SpdCas9-Dnmt3A/3L-V3

    Plasmid
    #222506
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V3 (R887E and E814G) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V3 (R887E and E814G) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; R887E and E814…
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222506-plasmid-map-sequence-id-441224

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  2. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid
    #112922
    Purpose
    Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker
    Depositor
    Kosuke Yusa
    Publication
    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    Use
    Gateway Cloning
    Expression
    Mammalian
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    112922-plasmid-map-sequence-id-217731

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  3. pCSD_2XNLS-SpCas9MT3-NLS-3XHA-2XNLS-NmdCas9-2XNLS

    Plasmid
    #107323
    Purpose
    Expresses SpCas9 (R1335K) fused to dNmCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-NmCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 (R1335K) is fused to nuclease dead NmCas9 …
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107323-plasmid-map-sequence-id-204717

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  5. Cas9-ryt

    Plasmid
    #246021
    Purpose
    Expresses Cas9 under control of Hsp70 promoter and gRNAs targeting Lctra, yellow, and RFPex
    Depositor
    Max Scott
    Inserts
    Cas9
    RFPex
    Use
    CRISPR
    Tags
    NLS
    Available since
    Jan. 29, 2026
    Availability
    Academic Institutions and Nonprofits only
    246021-plasmid-map-sequence-id-498404

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  6. Cas9-tra246

    Plasmid
    #246020
    Purpose
    Expresses Cas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene
    Depositor
    Max Scott
    Inserts
    Cas9
    RFPex
    Use
    CRISPR
    Tags
    NLS
    Available since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    246020-plasmid-map-sequence-id-494616

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  7. Cas9-tra135

    Plasmid
    #246019
    Purpose
    Expresses Cas9 under control of Hsp70 promoter and gRNAs targeting Lucilia cuprina transformer gene
    Depositor
    Max Scott
    Inserts
    Cas9
    RFPex
    Use
    CRISPR
    Tags
    NLS
    Available since
    Dec. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    246019-plasmid-map-sequence-id-494614

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  8. pCAG-scFvGCN4sfGFP-VP64-GB1

    Plasmid
    #141417
    Purpose
    Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation
    Depositor
    Izuho Hatada
    Publication
    Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    scFvGCN4sfGFP-VP64-GB1
    Tags
    HA and sfGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    141417-plasmid-map-sequence-id-300656

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  9. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

    Plasmid
    #112921
    Purpose
    Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker
    Depositor
    Kosuke Yusa
    Publication
    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo
    Use
    Gateway Cloning
    Expression
    Mammalian
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    112921-plasmid-map-sequence-id-217551

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  10. PB-CAG-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102885
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102885-plasmid-map-sequence-id-259794

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  11. PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102889
    Purpose
    PiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    102889-plasmid-map-sequence-id-198247

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  12. pAN1646

    Plasmid
    #127205
    Purpose
    pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS
    Depositor
    Hana El-Samad
    Publication
    Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586
    Insert
    p7x_tet-Venus-tADH1-pGal1-dCas9-VP64-Linker-mScarlet-Linker-degronSwitch_A_t12-tPGK1-pZ3-key_A_e18-mTagBFP2-NLS(SV40)-tSSA1
    Use
    Synthetic Biology
    Availability
    Academic Institutions and Nonprofits only
    127205-plasmid-map-sequence-id-250026

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  14. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

    Plasmid
    #202553
    Purpose
    Nontargeting control vector with TagBFP2
    Depositor
    Frederic Meunier
    Publication
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Mutation
    Puromycin-resistance gene in the pLV hU6-sgRNA hU…
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202553-plasmid-map-sequence-id-404692

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  16. TRE3GV-SpdCas9-Dnmt3A/3L-catΔ

    Plasmid
    #222508
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; C706A and R832…
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222508-plasmid-map-sequence-id-441225

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Showing: 1661 - 1680 of 2441 results