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3XFlag pCMV5-TOPO TAZ (∂304)

Plasmid

#24810

Depositor

Jeff Wrana

Publication

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Contains ONLY amino acids 1-304.

Available since

June 3, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
Xenopus APC-myc

Plasmid

#16686

Depositor

Barry Gumbiner

Publication

Vleminckx et al J Cell Biol. 1997 Jan 27. 136(2):411-20.

Insert

APC (apc.L Frog)

Use

Xenopus expression

Tags

Myc

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Lyso-B

Plasmid

#209873

Purpose

Dimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1

Depositor

Sarah Cohen

Publication

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP B

Tags

LAMP1

Expression

Mammalian

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLG026 (tmn)

Plasmid

#157904

Purpose

Expresses the tmn defense system

Depositor

Feng Zhang

Publication

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

tmn (transmembrane ATPase)

Expression

Bacterial

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-actin prom-dsRed-scramble shRNA

Plasmid

#67879

Purpose

AAV vector to express dsRed sh RNA (scramble)

Depositor

William Wisden

Publication

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

actin prom-dsRed-scramble shRNA

Use

AAV and RNAi

Expression

Mammalian

Promoter

chicken beta actin

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only
pLV-EF1A-CD19.8H.8TM.28.BB.3z

Plasmid

#200672

Purpose

Modular CD8hinge-CD8transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Publication

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD8HD-CD8TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TEVp

Plasmid

#162599

Purpose

TEV protease under CMV promoter

Depositor

Masaharu Somiya

Publication

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

TEV protease

Expression

Mammalian

Promoter

CMV promoter

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTE100

Plasmid

#59395

Purpose

MethyloBrick cloning vector, no promoter; TcR

Depositor

Tobias Erb

Publication

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

none

Available since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

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iLID-mCl3-MAP7N

Plasmid

#190167

Purpose

Expresses iLID-mCl3-MAP7N, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

Depositor

Anna Akhmanova

Publication

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

iLID-mClover3-MAP7N (MAP7 Human, Synthetic)

Tags

iLID-mClover3

Expression

Mammalian

Mutation

aa 1-309 from human MAP7 delta175-213

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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OPA1-isoform5

Plasmid

#70177

Purpose

Mammalian expression of hsOPA1 isoform 5

Depositor

David Chan

Publication

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 5 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)

Plasmid

#68218

Purpose

Module composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Luc / Renilla / P19

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos / 35S / 35S

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp2-a

Plasmid

#40047

Depositor

Fernando Martin-Belmonte

Publication

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a (SYTL2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV promoter

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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BL21ΔF

Bacterial strain

#102259

Purpose

ompF deletion strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Publication

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial resistance

None

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only
pETNb-nALFA

Plasmid

#159988

Purpose

For the protein expression with ALFA tag at N-terminus in E. coli

Depositor

Motoyuki Hattori

Publication

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

MVKYI-8histidine-ALFA tag-HRV3C protease cleavage…

Expression

Bacterial

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTagBFP-Akt1

Plasmid

#86594

Purpose

Expresses TagBFP-tagged human Akt1(WT)

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Expression

Mammalian

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-BD-cop1at(335-675)

Plasmid

#44973

Depositor

Thanos Halazonetis

Publication

Crefcoeur et al Nat Commun. 2013;4:1779. doi: 10.1038/nc

Insert

BD-COP1 (COP1 Mustard Weed)

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Mutation

deleted amino acids 1-334

Promoter

pCMV

Available since

June 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCR8-attL3-pegRNA-attL2

Plasmid

#213052

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2

Depositor

Bing Yang

Publication

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG MCP-VP64

Plasmid

#92357

Purpose

CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Publication

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

MCP-VP64

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-Rabin8

Plasmid

#24910

Depositor

Maxence Nachury

Publication

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

Rabin8 (RAB3IP Human)

Use

Gateway Cloning

Available since

July 7, 2010

Availability

Academic Institutions and Nonprofits only

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pRK5-myc-Miro1 S432N

Plasmid

#47893

Purpose

Expresses myc tagged Miro1 S432N dominant negative mutant

Depositor

Pontus Aspenström

Publication

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Insert

Miro1 S432N (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

S432N, dominant negative mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles