We narrowed to 20,348 results for: lic

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KCC2D_HUMAN_D0

Plasmid

#79734

Purpose

This plasmid encodes the kinase domain of KCC2D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2D

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
AAPK2_HUMAN_D0

Plasmid

#79738

Purpose

This plasmid encodes the kinase domain of AAPK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

AAPK2

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC4_HUMAN_D0

Plasmid

#79744

Purpose

This plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC4 (SLC12A7 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC1G_HUMAN_D0

Plasmid

#79745

Purpose

This plasmid encodes the kinase domain of KCC1G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC1G

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KCC2A_HUMAN_D0

Plasmid

#79746

Purpose

This plasmid encodes the kinase domain of KCC2A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KCC2A

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
KC1G2_HUMAN_D0

Plasmid

#79716

Purpose

This plasmid encodes the kinase domain of KC1G2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1G2

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
STK10_HUMAN_D0

Plasmid

#79722

Purpose

This plasmid encodes the kinase domain of STK10. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

STK10 (STK10 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
MK03_HUMAN_D0

Plasmid

#79688

Purpose

This plasmid encodes the kinase domain of MK03. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

MK03

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
PAK4_HUMAN_D0

Plasmid

#79698

Purpose

This plasmid encodes the kinase domain of PAK4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

PAK4 (PAK4 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
PAK6_HUMAN_D0

Plasmid

#79703

Purpose

This plasmid encodes the kinase domain of PAK6. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

PAK6 (PAK6 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pET30Xa-SBL1(160E,NoCys)

Plasmid

#66875

Purpose

Expresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160E) with all Cys to Ser mutations

Tags

Histag

Expression

Bacterial

Mutation

S160E; changed 4 (all) cysteines to serine

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pKSJ376

Plasmid

#103065

Purpose

Expresses colicin E1 with R domain deletion and ImmE1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Inserts

colicin E1 immunity protein (pColE1_26, imm E. coli)
cea/colicin E1

Expression

Bacterial

Mutation

R (receptor-binding domain) deletion in colicin E…

Promoter

T7 lac (from pET) and T7 lac (from pET52-b)

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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ATG-3925 (CBG2)

Plasmid

#167839

Purpose

Expresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cells

Depositor

Lance Encell

Article

Zambito et al iScience. 2020 Dec 26;24(1):101986. doi:

Insert

Click Beetle Green 2

Tags

6-His

Expression

Bacterial and Mammalian

Mutation

Q348H. Click Beetle Green 2 (CBR2) has 3 amino a…

Promoter

CMV, T7

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET-NPR4 (SA binding domain)

Plasmid

#226885

Purpose

Expresses the Salicylic acid binding core (SBC) of NPR4 in E.coli, in an insoluble form

Depositor

Ning Zheng

Article

Wang et al Nature. 2020 Oct;586(7828):311-316. doi:

Insert

The Salicylic acid (SA) binding domain of NPR4 (NPR4 Mustard Weed)

Tags

His tag

Expression

Bacterial

Mutation

NPR4 SA-binding core (residues 373-516), with a s…

Promoter

T7

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pKSJ375

Plasmid

#103064

Purpose

Expresses ImmE1, immunity to colicin E1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Insert

colicin E1 immunity protein (pColE1_26, imm E. coli)

Expression

Bacterial

Promoter

E1 immunity protein promoter from ColE1

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pTE1059

Plasmid

#186628

Purpose

Expression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.

Depositor

Eriko Takano

Article

Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10

Inserts

scbA
scbB
scbC

Use

Synthetic Biology

Tags

6xHis and 6xHis-thrombin

Expression

Bacterial

Promoter

Synthetic promoter B10, Synthetic promoter B19, a…

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMS1088

Plasmid

#80159

Purpose

pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

Depositor

Marcelo Sousa

Article

Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

Insert

HisSUMO-Cys-4xNuG2-Ald

Tags

HisSUMO

Promoter

T7

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMS1251

Plasmid

#80163

Purpose

pET-Duet for bacterial expression of Ald-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

Depositor

Marcelo Sousa

Article

Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

Insert

HisSUMO-Ald-4xNuG2-Ald

Tags

HisSUMO

Promoter

T7

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pET30Xa-SBL1(160E)

Plasmid

#66846

Purpose

Expresses soybean lipoxygenase-1 S160E mutant with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (LOC547694, NEWENTRY Glycine max)

Tags

His-tag

Expression

Bacterial

Mutation

S160E

Promoter

T7lac

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pET30-SBL1(160S,HisTagDel)

Plasmid

#66880

Purpose

Expresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removed

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160S)

Tags

none

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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