We narrowed to 65,142 results for: ang

1. pLX304-YAP1 _PDZ

   Plasmid

   #59147

   Purpose

   YAP1 mutants that harbored a deletion of the 5 amino acids

   Depositor

   William Hahn

   Article

   Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   PDZ binding domain (last 5aa) deleted

   Promoter

   CMV

   Available since

   Oct. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCAG-FLEX-fwd[Chrimson-tdT]

   Plasmid

   #59137

   Purpose

   Expression vector for Chrimson-tdTomato in a floxed/forward-reading, Cre-dependent manner (Cre turns gene off)

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chrimson-tdTomato
   

   Use

   Cre/Lox

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOTTC588 - pAAV c-fos Nuc-iRFP

   Plasmid

   #59132

   Purpose

   An AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Nuclear-localized Infrared Fluorescent Protein
   

   Use

   AAV

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   mFos

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lentiCRISPR - EGFP sgRNA 3

   Plasmid

   #51762

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PGL4-4X TE Down (GQs) Renilla

   Plasmid

   #70039

   Purpose

   Plasmid for testing 5’UTR TE Down (G-quadruplex) folding elements

   Depositor

   Hans-Guido Wendel

   Article

   Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

   Insert

   4x TE Down (G-quadruplexes) in 5'UTR
   

   Use

   Luciferase

   Tags

   renilla luciferase

   Expression

   Mammalian

   Mutation

   4 repeats of TE down motif (G-quadruplexes) in 5&…

   Promoter

   SV40

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pMCSF-R(mB)-luc

   Plasmid

   #12422

   Depositor

   Dong-Er Zhang

   Article

   Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

   Insert

   M-CSF receptor promoter fragment (CSF1R Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   AML1 binding site mutation (created a KpnI site i…

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pCLXSN(GFP)-HA-Slc1a5 (mouse)

   Plasmid

   #71458

   Purpose

   inducible expression fo Slc1a5 in mammalian cells

   Depositor

   Shao-Cong Sun

   Article

   Nakaya et al Immunity. 2014 May 15;40(5):692-705. doi

   Insert

   Slc1a5 (Slc1a5 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   A85P, L125F and V384A mutations compared to GenBa…

   Promoter

   CMV

   Available since

   Jan. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

8. lentiCRISPR - EGFP sgRNA 6

   Plasmid

   #51765

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 6
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLX304-YAP1 _SH3bm

   Plasmid

   #59141

   Purpose

   YAP1 mutants that harbored a deletion of SH3 binding motif, which disrupts interaction of YES1 with YAP1

   Depositor

   William Hahn

   Article

   Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   SH3 domain deleted (aa278-290)

   Promoter

   Available since

   Oct. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

10. FLAG-EglN2 H358A-pBabe-puro

    Plasmid

    #22705

    Depositor

    William Kaelin

    Article

    Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

    Insert

    Egg laying Nine - 2 (EglN2) (EGLN2 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    mutated cDNA: point mutation in codon 358 changi…

    Promoter

    Available since

    Dec. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMCSF-R(mA)-luc

    Plasmid

    #12421

    Depositor

    Dong-Er Zhang

    Article

    Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

    Insert

    M-CSF receptor promoter fragment (CSF1R Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    C/EBP binding site mutation (created a KpnI site …

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

12. J7AAV-HDR Fah.2

    Plasmid

    #73451

    Purpose

    J7AAV-HDR U6sgFah.2 template2 to repair Fah mutation

    Depositor

    Wen Xue

    Article

    Yin et al Nat Biotechnol. 2016 Feb 1. doi: 10.1038

    Insert

    Fah (Fah Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. J8AAV-HDR Ctnnb1.1

    Plasmid

    #73452

    Purpose

    J8AAV-HDR U6sgCtnnb1.1 template

    Depositor

    Wen Xue

    Article

    Yin et al Nat Biotechnol. 2016 Feb 1. doi: 10.1038

    Insert

    Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-N-Flag-ASC1

    Plasmid

    #75134

    Purpose

    Expresses N-terminal Flag-tagged ASC1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    PYD and CARD domain containing (Pycard Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F23S (please see depositor comments below)

    Promoter

    CMV

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOTTC374 - pAAV EF1a DIO iRFP

    Plasmid

    #47626

    Purpose

    An AAV vector that expresses iRFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    Infrared fluorescent protein
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid

    #85452

    Purpose

    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.

    Depositor

    Aviv Regev

    Article

    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do

    Insert

    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOTTC469 - pAAV EF1a DIO eNpHR3.0-iRFP

    Plasmid

    #47631

    Purpose

    An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    eNpHR3.0
    

    Use

    AAV and Cre/Lox

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEM-GFP-URA3-GFP

    Plasmid

    #72606

    Purpose

    Template for creating a C-terminal GFP tag AND an internal GFP tag from a single transfection of yeast

    Depositor

    Bruce Granger

    Article

    Granger et al PLoS One. 2018 Jan 12;13(1):e0191194. do

    Inserts

    GFP (full)
    URA3
    GFP (partial)
    Optional Linker
    

    Use

    Tags

    Expression

    Mutation

    Includes full 819 bp coding sequence of URA3, 435…

    Promoter

    Available since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOTTC352 - pAAV CaMKIIa eNpHR3.0-iRFP

    Plasmid

    #47904

    Purpose

    An AAV vector that expresses engineered halorhodopsin 3.0 (fused to iRFP) under the CaM Kinase IIa promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    eNpHR3.0
    

    Use

    AAV

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-N-Flag-NLRP3 1-90

    Plasmid

    #75137

    Purpose

    Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 1-90

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CA-RIT-NFAT1 (IRES-GFP)

    Plasmid

    #85181

    Purpose

    Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interaction. Co-expresses EGFP for selection

    Depositor

    Anjana Rao

    Article

    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

    Insert

    CA-RIT-NFAT1 (Nfatc2 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Amino acids 1-3 removed; CA: PASSGSSASF mutated t…

    Promoter

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3-N-HA-NEK7 K64M

    Plasmid

    #75143

    Purpose

    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lys 64 mutated to Met; kinase inactive and F236L …

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBS-EF1-IRES-NEO-Control

    Plasmid

    #80757

    Purpose

    Transposon Reporter

    Depositor

    Alex Kentsis

    Article

    Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

    Insert

    NeoR (neoR Synthetic)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    EF1-IRES from PB-EF1-IRES-NEO

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CA-RIT-NFAT1 (PGK-Puro)

    Plasmid

    #63214

    Purpose

    Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interactio. Contains Puromycin selection marker

    Depositor

    Anjana Rao

    Article

    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

    Insert

    CA-RIT-NFAT1 (Nfatc2 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Amino acids 1-3 removed; CA: PASSGSSASF mutated t…

    Promoter

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

25. MIG-eIF4A1 P159E

    Plasmid

    #70042

    Purpose

    Expression of eIF4A1 P159E mutant construct in mammalian cells.

    Depositor

    Hans-Guido Wendel

    Article

    Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

    Insert

    eIF4A1 (Eif4a1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    P159E

    Promoter

    CMV

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pAAV-EF1α-FLEX-rc[Chronos-GFP]

    Plasmid

    #62725

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MIG-eIF4A1 F163L

    Plasmid

    #70043

    Purpose

    Expression of eIF4A1 F163L mutant in mammalian cells

    Depositor

    Hans-Guido Wendel

    Article

    Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

    Insert

    eIF4A1 (Eif4a1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    F163L

    Promoter

    CMV

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

28. MIG-eIF4A1 Q195E

    Plasmid

    #70044

    Purpose

    Expression of eIF4A1 Q195E mutant in mammalian cells

    Depositor

    Hans-Guido Wendel

    Article

    Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

    Insert

    eIF4A1 (Eif4a1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Q195E

    Promoter

    CMV

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

29. Fgf4enhLV (FpG12)

    Plasmid

    #69444

    Purpose

    positive control plasmid for lentiviral reporter expression in ESCs

    Depositor

    Lisa Dailey

    Article

    Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

    Inserts

    minimal FGF4 promoter
    EGFP
    Ubiquitin promoter
    Hygromycin resistance
    FGF4 enhancer (Fgf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTH734-2µ-RLuc/stopCFLuc

    Plasmid

    #40609

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase (nonsense mutant)
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP

    Plasmid

    #47905

    Purpose

    An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) under the CaM Kinase IIa promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    iRFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CaMKIIa

    Available since

    Sept. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV5-Flag-Ago2-FDM3

    Plasmid

    #72210

    Purpose

    Mammalian expression of aa 227-860 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 1-226

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

33. pCMV5-Flag-Ago2-FDM1

    Plasmid

    #72208

    Purpose

    Mammalian expression of aa 1-372 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 373-860

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

34. pCMV5-Flag-Ago2-FDM2

    Plasmid

    #72209

    Purpose

    Mammalian expression of aa 1-517 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 518-860

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

35. pCMV5-Flag-Ago2-FDM4

    Plasmid

    #72211

    Purpose

    Mammalian expression of aa 373-860 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 1-372

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

36. pCMV5-Flag-Ago2-FDM5

    Plasmid

    #72212

    Purpose

    Mammalian expression of aa 518-860 of human Ago2

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    argonaute RISC catalytic component 2 (AGO2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Δ 1-517

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

37. XE130 beta2AR Fz2-Cs2+

    Plasmid

    #16794

    Depositor

    Randall Moon

    Article

    Liu et al Proc Natl Acad Sci U S A. 1999 Dec 7;96(

    Insert

    beta 2 AR Fz2 (Fzd1, Adrb2 Rat)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

38. pTH752-CEN-RLuc/min103maxCFLuc

    Plasmid

    #38218

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The first 103 codons of the original FLuc gene we…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTH751-CEN-RLuc/min53maxCFLuc

    Plasmid

    #38217

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The first 53 codons of the original FLuc gene wer…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTH748-CEN-RLuc/min8maxCFLuc

    Plasmid

    #38214

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The first eight codons of the original FLuc gene …

    Promoter

    ADH1 and TDH3 (=GDP)

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pTH753-CEN-RLuc/min346maxCFLuc

    Plasmid

    #38219

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The first 346 codons of the original FLuc gene we…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pTH747-CEN-RLuc/min4maxCFLuc

    Plasmid

    #38213

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The first four codons of the original FLuc gene w…

    Promoter

    ADH1 and TDH3 (=GDP)

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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43. pTnsABC

    Plasmid

    #160031

    Purpose

    Expresses V. cholerae TnsABC

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTnsA, VchTnsB, VchTnsC
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    no

    Promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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44. pCutamp

    Plasmid

    #140632

    Purpose

    Plasmid-curing in Escherichia coli by targeting the AmpR promoter

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pQCascade

    Plasmid

    #140622

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and CRISPR RNA

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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46. pSC101-Donor

    Plasmid

    #140630

    Purpose

    Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    LE, RE, KanR, temperature sensitive pSC101 origin of replication
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pHelper-T7IS1

    Plasmid

    #140631

    Purpose

    Expresses ShCAST under the T7 promoter and an empty sgRNA

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pDonor-GDH

    Plasmid

    #140629

    Purpose

    Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    Mini-Tn6677, T7lac-GDH gene cassette
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pQCascade-array1

    Plasmid

    #140623

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-array1. The crRNA-array1 targets 8 different loci in Escherchia coli.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-array1
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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50. pQCascade-IS1

    Plasmid

    #140624

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS1. The crRNA-IS1 targets IS1 loci in Escherchia coli.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS1
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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