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pKAR2-Br512_Mut235

Plasmid

#179278

Purpose

Expresses Br512_Mut235 polymerase in E.coli

Depositor

Andrew Ellington

Publication

Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

Insert

Engineered Bst DNA Polymerase I Large Fragment

Tags

8x His tag; Villin Headpiece 47 amino acid (HP47)…

Expression

Bacterial

Promoter

T7

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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p2lox-cAMPr R739A

Plasmid

#102618

Purpose

cAMPr with a point mutation R739A which theoretically increases its affinity for cAMP

Depositor

Justin Blau

Publication

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr

Use

Cre/Lox

Expression

Mammalian

Mutation

R739A

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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M4786 KanMX::hisG-URA3-HisG Disruptor Converter

Plasmid

#51690

Purpose

Yeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisG

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

hisG-URA3-hisG (URA3 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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Mumps V-FLAG

Plasmid

#44908

Depositor

Curt Horvath

Publication

Ulane et al J Virol. 2003 Jun;77(11):6385-93.

Insert

Mumps V (V/P )

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pJWZ6

Plasmid

#21744

Depositor

David Sherwood

Publication

Ziel et al Nat Cell Biol. 2009 Feb . 11(2):183-9.

Insert

mCherry::dMoeABD

Use

Bacterial propagation

Tags

mCherry

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV:ECFP(FRT)EYFP

Plasmid

#31373

Depositor

Gerhart Ryffel

Publication

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

ECFP(FRT)EYFP

Use

Flp/frt

Expression

Mammalian

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk3a1

Plasmid

#15745

Depositor

Roger Davis

Publication

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a1 (MAPK10 Human)

Expression

Bacterial

Available since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
pEsg1-6K-Luc

Plasmid

#15394

Depositor

Shinya Yamanaka

Publication

Amano et al BMC Dev Biol. 2006 Feb 28;6:11. doi: 10.

Insert

Upstream region of mouse Esg1 gene (Dppa5a Mouse)

Use

Luciferase

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pMRX-IZ-ANXA6-mRuby3

Plasmid

#184892

Purpose

Stable expression of ANXA6-mRuby3 (Annexin A6-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Publication

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA6 (ANXA6 Human)

Tags

mRuby3

Expression

Mammalian

Mutation

R587K

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-ANXA2-mRuby3

Plasmid

#184889

Purpose

Stable expression of ANXA2-mRuby3 (Annexin A2-mRuby3) in mammalian cells

Depositor

Noboru Mizushima

Publication

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

ANXA2 (ANXA2 Human)

Tags

mRuby3

Expression

Mammalian

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-PIK3CG

Plasmid

#174189

Purpose

Expresses PIK3CG in mammalian cells

Depositor

Cheng-han Yu

Publication

Zhou et al J Cell Sci. 2020 Jun 8;133(11). pii: jcs

Insert

PIK3CG (PIK3CG Human)

Tags

EGFP

Expression

Mammalian

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pMT GFP

Plasmid

#126657

Purpose

Inducible expression of GFP in S2 cells

Depositor

Ron Vale

Publication

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

GFP

Expression

Insect

Available since

Dec. 7, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry_LD 0_TEV

Plasmid

#58868

Purpose

MESA protease chain with mCherry ectodomain and no linker domain

Depositor

Joshua Leonard

Publication

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with mCherry ectodomain and TEV protease

Use

AAV

Expression

Mammalian

Promoter

CMV

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pALACE

Plasmid

#31853

Depositor

Yossef Av-Gay

Publication

Newton et al J Bacteriol. 2003 Jun;185(11):3476-9.

Type

Empty backbone

Use

Escherichia coli-mycobacterium shuttle plasmid

Tags

6xHIS

Expression

Bacterial

Promoter

Acetamidase operone

Available since

July 9, 2012

Availability

Academic Institutions and Nonprofits only
FL-fibulin-3-V5-lentiviral-GFP

Plasmid

#29703

Depositor

Mariano Viapiano

Publication

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

EFEMP1 (EFEMP1 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Available since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only
pEA186

Plasmid

#187874

Purpose

Empty pVS1-based INTEGRATE vector with mCherry cargo

Depositor

Kan Wang

Publication

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMSP1E2D1

Plasmid

#161606

Purpose

Bacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formation

Depositor

Stephen Sligar

Publication

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E2D1

Tags

7-His

Expression

Bacterial

Mutation

"extended" MSP1D1; contains repeat of h…

Promoter

T7

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pR26-CAG-H2B-miRFP703

Plasmid

#112853

Purpose

Donor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell lines

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

H2B-miRFP703

Expression

Mammalian

Promoter

CAG

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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TOP-H2B-YFP-DD

Plasmid

#96891

Purpose

Reporter to monitor translation of 5'TOP mRNA

Depositor

Tobias Meyer

Publication

Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

Insert

TOP-H2B-YFP-DD

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1 3xF-dyskerin

Plasmid

#126870

Purpose

Expresses 3xFlag dsykerin

Depositor

Kathleen Collins

Publication

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

Dyskerin

Tags

3xFlag

Expression

Mammalian

Available since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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