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pmr101A-hVPS26

Plasmid

#17636

Depositor

James Hurley

Article

Shi et al Nat Struct Mol Biol. 2006 Jun . 13(6):54

Insert

VPS26 (VPS26A Human)

Tags

His

Expression

Bacterial

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagC

Plasmid

#99718

Purpose

Mammalian expression of HA-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagC (RRAGC )

Tags

HA

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-nLuc

Plasmid

#199755

Purpose

Control construct of elongation reporter to quantify elongation of YFP

Depositor

Brian Zid

Article

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

Nanoluc

Expression

Yeast

Promoter

TetO7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-AP-Ub

Plasmid

#232577

Purpose

Express Ubiquitin tagged with an Acceptor Peptide (AP)

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AviTag

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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Mito-V5-BS2 (Mitochondrial matrix)

Plasmid

#220344

Purpose

Mammalian expression vector BS2 in mitocondria

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

MTS-matrix and V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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bRA66

Plasmid

#100952

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Flag-SmBiT-PP1b

Plasmid

#224895

Purpose

Mammalian expression of Flag-SmBiT-PP1b

Depositor

Mathieu Bollen

Article

Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

Insert

Flag-SmBiT-PP1b

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-hTRIM9

Plasmid

#51032

Purpose

entry vector for cloning human TRIM9

Depositor

Timothy Cox

Article

Short et al J Biol Chem. 2006 Mar 31;281(13):8970-80

Insert

TRIM9 (TRIM9 Human)

Use

Entry vector

Promoter

NA

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pFUW COX8ASig-Lemon

Plasmid

#239560

Purpose

Expression and mitochondrial localization of Lemon, a pH biosensor

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

Lemon

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pPAmKate-N1

Plasmid

#34587

Depositor

Vladislav Verkhusha

Article

Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

Insert

PAmKate

Expression

Mammalian

Mutation

F84W/S148N/S165G compared to mKate (but numbering…

Promoter

CMV

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only

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pQCXIP-EGFP-APEX2-V5-Giantin

Plasmid

#236404

Purpose

Expresses a fluorescent Golgi-localized APEX2

Depositor

Jared Rutter

Article

Cunningham et al J Cell Sci. 2025 Mar 13:jcs.263693. doi:

Insert

EGFP-APEX2-V5-Giantin

Use

Retroviral

Tags

APEX2, EGFP, and V5

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHR Lck-TagBFP

Plasmid

#171034

Purpose

TagBFP-tagged Lck kinase

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

Lck

Use

Lentiviral

Expression

Mammalian

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-ArcLight-ST

Plasmid

#182318

Purpose

Fluorescent voltage indicator with left-shifted voltage-dependency

Depositor

Rafael Yuste

Article

Bando et al Nat Commun. 2021 Dec 10;12(1):7229. doi:

Insert

ArcLight-ST

Expression

Mammalian

Mutation

Mutation in an amino acid Q217E of ArcLight-MT

Promoter

CAG

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW mCherry-P2A-NeoR

Plasmid

#239570

Purpose

Cytosolic expression of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pH2B-LSSmKate2-N1

Plasmid

#31903

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

H2B-LSSmKate2

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

Promoter

CMV

Available Since

Sept. 20, 2011

Availability

Academic Institutions and Nonprofits only

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isce rfp/cfp

Plasmid

#83323

Purpose

fluorescent Isce joint substrate

Depositor

Katheryn Meek

Article

Neal et al J Immunol. 2016 Apr 1;196(7):3032-42. do

Insert

RFP +CFP joining substrate

Use

Synthetic Biology

Available Since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pMulti_S_ccdB

Plasmid

#223853

Purpose

GEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Type

Empty backbone

Use

Golden-gate acceptor

Expression

Bacterial

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJW1662

Plasmid

#112050

Purpose

C-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression cassette marked with LEU

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Inserts

IAA17
TIR1

Tags

HA

Expression

Yeast

Mutation

only amino acids 71-114

Promoter

ADH1 and None

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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GST-PRMT6

Plasmid

#34700

Depositor

Steven Clarke

Article

Frankel et al J Biol Chem. 2002 Feb 1;277(5):3537-43.

Insert

PMRT6

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-BRIC

Plasmid

#172341

Purpose

AAV transfer plasmid for BRIC (Bioluminescent Red Indicator for Calcium)

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BRIC

Use

AAV

Promoter

hSyn promoter

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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