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pCFD3-MS2tail-Scaffold

Plasmid

#78906

Purpose

Vector for expressing a single sgRNA with Scaffold MS2 tail, under U6:3 promoter

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available Since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z04

Plasmid

#120047

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mTFP1

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mTFP1

Use

Pcr template

Tags

mTFP1

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P30

Plasmid

#120041

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-G

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

VSV-G

Use

Pcr template

Tags

VSV-G

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBW214J115-Amp32C

Plasmid

#78649

Purpose

pSB3K3 carrying J115-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J115-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pMaCTag-25

Plasmid

#120004

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-19

Plasmid

#124787

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBW211J109-Amp32C

Plasmid

#78646

Purpose

pSB3K3 carrying J109-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J109-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pMaCTag-P03

Plasmid

#120014

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBW213J116-Amp32C

Plasmid

#78648

Purpose

pSB3K3 carrying J116-30hrpR-32hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

J116-30hrpR-32hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3_3_TOPO_TA-ms2-VP64

Plasmid

#79371

Purpose

Recruits VP64 to a compatible gRNA for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2-VP64

Use

CRISPR

Expression

Mammalian

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAct:MCP-VP64

Plasmid

#78904

Purpose

Expresses MCP:VP64 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2:VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

March 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-NLS-BioID2-HA

Plasmid

#232025

Purpose

mammalian expression (CMV promoter) of nuclear targeted BioID2-HA

Depositor

Primo Schaer

Article

Richina et al Cell Mol Life Sci. 2025 Nov 25. doi: 10.

Insert

NLS

Tags

NLS

Expression

Mammalian

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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GST-Thrombin-Munc13-1 MUN domain (aa859-1516 delta1408-1452 EF)

Plasmid

#243818

Purpose

Munc13-1 protein purification

Depositor

Jose Rizo-Rey

Article

Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

Insert

Munc13-1 (Unc13a Rat)

Tags

GST

Expression

Bacterial

Mutation

1408-1452 --> EF

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDLterm

Plasmid

#211903

Purpose

Base plasmid for dual-luciferase constructs to measure terminator strength

Depositor

Stan Fields

Article

Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

Type

Empty backbone

Use

Luciferase

Expression

Plant

Promoter

35S enhancer + 35S minimal promoter

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsUPF2-siRNAres_I

Plasmid

#146573

Purpose

Mammalian Expression of HsUPF2-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsUPF2-siRNAres (UPF2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1127B

Plasmid

#190997

Purpose

Expresses arrays of gRNA targeting rel1 promoter under U6b/c promoter

Depositor

Omar Akbari

Article

Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

Insert

U6b:sgRNArel1A1 - U6c:sgRNArel1A2 - U6b:sgRNArel1A3 - U6c:sgRNArel1A4

Expression

Insect

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_mcherry

Plasmid

#167894

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
NAX_CAGGS_CAS9_m4_mcherry

Plasmid

#167870

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
pGP8GF-ZmAFB2/3 b2

Plasmid

#188425

Purpose

Zea Mays Nuclear Auxin Signaling Receptor AFB2/3 b2

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor AFB2/3 b2

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGP8GF-ZmAFB2/3 a

Plasmid

#188423

Purpose

Zea Mays Nuclear Auxin Signaling Receptor AFB2/3 A

Depositor

Britney Moss

Article

Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

Insert

Auxin Signaling Receptor AFB2/3 a

Expression

Yeast

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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