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pCSII-EF-miRFP709-hCdt(1/100)

Plasmid

#80007

Purpose

hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hCdt1(1/100) (CDT1 Human)

Use

Lentiviral

Tags

miRFP709

Promoter

EF-1 alpha

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTXB1-nbMmIgG1-Tn5

Plasmid

#184287

Purpose

Anti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

Depositor

New York Genome Center, Ivan Raimondi

Publication

Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

Insert

nbMmIgG1-Tn5

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCherry- SEC61B

Plasmid

#121160

Purpose

Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Publication

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SEC61B (SEC61B )

Expression

Mammalian

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby

Plasmid

#71761

Purpose

Expression of membrane-tethered EGFP and Synaptophysin-mRuby from Flp-expressing neurons

Depositor

Liqun Luo

Publication

Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

Inserts

EGFP
Synaptophysin

Use

AAV

Tags

Palmitoylation signal and mRuby

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLH-sgRNA1-2XPP7

Plasmid

#75390

Purpose

sgRNA1-2XPP7

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMaRSC

Plasmid

#89189

Purpose

The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.

Depositor

David Tirrell

Publication

Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Flag-L274G-T2A-mCherry (Mars1 Mouse)

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRN1120

Plasmid

#101750

Purpose

Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

Depositor

Rene Verwaal

Publication

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Type

Empty backbone

Expression

Yeast

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTXB1-nbMmIgG1-Tn5

Plasmid

#184287

Purpose

Anti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

Depositor

New York Genome Center, Ivan Raimondi

Publication

Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

Insert

nbMmIgG1-Tn5

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCSII-EF-miRFP709-hCdt(1/100)

Plasmid

#80007

Purpose

hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hCdt1(1/100) (CDT1 Human)

Use

Lentiviral

Tags

miRFP709

Promoter

EF-1 alpha

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pSR43.6r

Plasmid

#63197

Purpose

Expresses CcaS, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Publication

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pJK02

Plasmid

#105133

Purpose

Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

Depositor

Joseph Sorg

Publication

McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

Inserts

Upstream & Downstream pyrE deletion region
tetR
Cas9
gRNA

Use

E. coli - c. difficile shuttle vector

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMitoLOC

Plasmid

#58980

Purpose

Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

Depositor

Markus Ralser

Publication

Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

Inserts

mCherry
GFP

Tags

preCOX4 and preSU9

Expression

Yeast

Promoter

ADH1 and MET17

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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mCherry- SEC61B

Plasmid

#121160

Purpose

Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Publication

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SEC61B (SEC61B )

Expression

Mammalian

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby

Plasmid

#71761

Purpose

Expression of membrane-tethered EGFP and Synaptophysin-mRuby from Flp-expressing neurons

Depositor

Liqun Luo

Publication

Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

Inserts

EGFP
Synaptophysin

Use

AAV

Tags

Palmitoylation signal and mRuby

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pYPQ150

Plasmid

#69301

Purpose

Gateway entry vector with pcoCas9 (Plant codon optimized)

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9 (plant codon-optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

NLS fusion

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSR43.6r

Plasmid

#63197

Purpose

Expresses CcaS, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Publication

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pJK02

Plasmid

#105133

Purpose

Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

Depositor

Joseph Sorg

Publication

McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

Inserts

Upstream & Downstream pyrE deletion region
tetR
Cas9
gRNA

Use

E. coli - c. difficile shuttle vector

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pY026

Plasmid

#84741

Purpose

Expresses huAsCpf1 and crRNA guide

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

huAsCpf1

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMitoLOC

Plasmid

#58980

Purpose

Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

Depositor

Markus Ralser

Publication

Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

Inserts

mCherry
GFP

Tags

preCOX4 and preSU9

Expression

Yeast

Promoter

ADH1 and MET17

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET-His6-ERK2-MEK1_R4F_coexpression

Plasmid

#39212

Depositor

Melanie Cobb

Publication

Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

Inserts

Mek1 R4F (MAP2K1 Human)
Erk2 (Mapk1 Rat)

Tags

His6 and RBS

Expression

Bacterial

Mutation

MEK1(R4F) = S218E, S222D and deletion of AA32-51

Promoter

T7 and T7 via RBS

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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