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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 77,003 results for: rest

Showing: 1681 - 1700 of 77003 results

  1. pOpen-HindIIIR

    Plasmid
    #165508
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence AAGCTT and cleaves after A-1.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    HindIII
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165508-plasmid-map-sequence-id-338587

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  2. pOpen-NcoIR

    Plasmid
    #165568
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence CCATGG and cleaves after C-1.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    NcoI
    Use
    Synthetic Biology
    Available Since
    Aug. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165568-plasmid-map-sequence-id-340100

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  3. pRS414-Fyv6-CFLAG

    Plasmid
    #234133
    Purpose
    Restriction digest from pUC18-CFLAG-Fyv6 and ligation into pRS414
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234133-plasmid-map-sequence-id-466523

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  4. pRS414-NFLAG-Fyv6

    Plasmid
    #234139
    Purpose
    Restriction digest from pUC18-NFLAG-Fyv6 and ligation into pRS414
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234139-plasmid-map-sequence-id-466518

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  5. pUltra-Plus

    Plasmid
    #161775
    Purpose
    3rd generation lentiviral multicistronic vector for mammalian expression of three genes of interest
    Depositor
    Jorge Luis Arias-Arias
    Type
    Empty backbone
    Use
    Lentiviral; Multicistronic
    Expression
    Mammalian
    Promoter
    UbC
    Available Since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161775-plasmid-map-sequence-id-316822

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  6. pOpen-Eco31I

    Plasmid
    #165506
    Purpose
    Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    Eco31I
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165506-plasmid-map-sequence-id-338512

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  7. pUC-L3L2

    Plasmid
    #114019
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114019-plasmid-map-sequence-id-222772

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  8. pUC-L1L4

    Plasmid
    #114018
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114018-plasmid-map-sequence-id-222765

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  9. pUC-L1L2

    Plasmid
    #114017
    Purpose
    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning
    Depositor
    Christopher Grefen
    Insert
    ccdB gene, Chloramphenicol resistance gene
    Use
    Gateway
    Available Since
    Jan. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    114017-plasmid-map-sequence-id-222768

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  10. pOpen-NotIR (GC)

    Plasmid
    #165507
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    NotI (GC-rich recognition site)
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165507-plasmid-map-sequence-id-338586

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  11. 371-A

    Plasmid
    #97026
    Purpose
    yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    97026-plasmid-map-sequence-id-182505

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  12. pOpen-SfiIR (cDNA)

    Plasmid
    #165528
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    SfiI (used to create cDNA libraries)
    Use
    Synthetic Biology
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165528-plasmid-map-sequence-id-339067

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  13. pLenti-sgRNA

    Plasmid
    #71409
    Purpose
    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
    Depositor
    Eric Lander, David Sabatini
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71409-plasmid-map-sequence-id-322475

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  15. Flag-APEX3-C Cloning Vector

    Plasmid
    #220615
    Purpose
    To express a protein of interest fused to the C-terminus of Flag-APEX3 for proximity labeling or TEM (unpublished)
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Tags
    Flag-APEX3 fusion
    Expression
    Mammalian
    Available Since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    220615-plasmid-map-sequence-id-435941

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  16. pSLfa[Tc'hsp5'3'UTR]fa

    Plasmid
    #86450
    Purpose
    Heat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)
    Depositor
    Gregor Bucher
    Insert
    Tc'hsp5'3'UTR
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86450-plasmid-map-sequence-id-171108

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  17. Flag-2xAPEX2-C Cloning Vector

    Plasmid
    #136991
    Purpose
    To express a protein of interest fused to the C-terminus of Flag-2xAPEX2 for proximity labeling or TEM
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    136991-plasmid-map-sequence-id-271567

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  18. 371-U

    Plasmid
    #97036
    Purpose
    MYFQSNA-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Tags
    MYFQSNA
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    97036-plasmid-map-sequence-id-182502

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  19. pDEST-Spe4-Kozak-miRFP713-RfA

    Plasmid
    #186381
    Purpose
    Destination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    May 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    186381-plasmid-map-sequence-id-392698

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  20. pDEST-Spe4-RfA-miRFP713-stop

    Plasmid
    #186391
    Purpose
    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjection
    Depositor
    Patrick Lemaire
    Type
    Empty backbone
    Use
    Gateway destination vector
    Available Since
    Oct. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    186391-plasmid-map-sequence-id-373838

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Showing: 1681 - 1700 of 77003 results