Skip to main content
Addgene
Showing: 4251 - 4300 of 17876 results
  1. pYTK086

    Plasmid
    #65193
    Purpose
    Encodes URA3 3' Homology as a Type 7 part to be used in the Dueber YTK system
    Depositor
    Insert
    URA3 3' Homology
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pFA6a-HIS3MX6-PGAL1-GFP (F64L, S65T, V163A)

    Plasmid
    #53207
    Purpose
    N-terminal chromosomal tagging with stable GFP under control of GAL promoter
    Depositor
    Insert
    GAL1 promoter (GAL1 Budding Yeast)
    Use
    Yeast genomic targeting
    Tags
    GFP (F64L, S65T, V163A) and HIS3MX6
    Expression
    Mutation
    Promoter
    GAL1 promoter
    Available since
    May 27, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. pYTK082

    Plasmid
    #65189
    Purpose
    Encodes 2micron as a Type 7 part to be used in the Dueber YTK system
    Depositor
    Insert
    2micron
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. pYTK093

    Plasmid
    #65200
    Purpose
    Encodes LEU2 5' Homology as a Type 8b part to be used in the Dueber YTK system
    Depositor
    Insert
    LEU2 5' Homology
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  5. pYTK087

    Plasmid
    #65194
    Purpose
    Encodes LEU2 3' Homology as a Type 7 part to be used in the Dueber YTK system
    Depositor
    Insert
    LEU2 3' Homology
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. FC512

    Plasmid
    #27331
    Depositor
    Insert
    CDC28 (CDC28 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    rsc1 mutation in CDC28 (R159G)
    Promoter
    Available since
    Feb. 28, 2011
    Availability
    Academic Institutions and Nonprofits only
  7. Sc Pol3-FLAG(Ec)-pRSF405/GAL

    Plasmid
    #239198
    Purpose
    Overexpress Sc Pol3(C-terminal 3X FLAG tag) when integrated into yeast (S. cer)
    Depositor
    Insert
    Pol3 (POL3 Budding Yeast)
    Use
    Tags
    3X FLAG
    Expression
    Yeast
    Mutation
    E. coli codon bias (synthetic gene), but same ami…
    Promoter
    Available since
    June 4, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. TK4-NLS-EGFP

    Plasmid
    #239046
    Purpose
    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation
    Depositor
    Inserts
    EGFP
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2 and c-myc-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and EF1a
    Available since
    May 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. CPH3737_GST-TEV-Spt6p-tSH2-1223-1453

    Plasmid
    #227087
    Purpose
    Bacterial expression of K1355A/K1435A double-mutant GST-tagged Spt6 tSH2 domain (1223-1453)
    Depositor
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Use
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    K1355A, K1435A
    Promoter
    T7
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
  10. pAG416-GAL-SUP35

    Plasmid
    #181705
    Purpose
    Galactose inducible expression of SUP35
    Depositor
    Insert
    SUP35 (SUP35 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. pLS406

    Plasmid
    #231078
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3
    Depositor
    Type
    Empty backbone
    Use
    Integrating
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Feb. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
  12. pLS405

    Plasmid
    #231077
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker LEU2
    Depositor
    Type
    Empty backbone
    Use
    Integrating
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
  13. pLS404

    Plasmid
    #231076
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker TRP1
    Depositor
    Type
    Empty backbone
    Use
    Integrating
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
  14. pLS408

    Plasmid
    #231079
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker natMX6
    Depositor
    Type
    Empty backbone
    Use
    Integrating
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
  15. pLS403

    Plasmid
    #231075
    Purpose
    Minimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker HIS3
    Depositor
    Type
    Empty backbone
    Use
    Integrating
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
  16. CPH3747_GST-TEV-Spt6p-tSH2-1223-1452

    Plasmid
    #227086
    Purpose
    Bacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)
    Depositor
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Use
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    R1282H
    Promoter
    T7
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  17. CPH3375_SF_Spt6p-tSH2-1223-1451_NLS

    Plasmid
    #227084
    Purpose
    Gal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus
    Depositor
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Use
    Tags
    STREPii-FLAG and SV40 NLS 1
    Expression
    Yeast
    Mutation
    Promoter
    Gal1-10
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. CUP1-∆ssCPY*-mScarletI

    Plasmid
    #221157
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 4251 - 4300 of 17876 results