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V180 pLP Triple-Flag SD - Acceptor

Plasmid

#11707

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

3xFLAG

Expression

Mammalian

Available Since

June 22, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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EF1a_KLF3_P2A_Hygro_Barcode

Plasmid

#120490

Purpose

Barcoded lentiviral vector to express KLF3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF3 (KLF3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_NADP-Snifit

Plasmid

#113913

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pRSETB-de4GFP

Plasmid

#85472

Purpose

Inducible expression of dual emitting pH sensitive GFP in bacterial cells

Depositor

S. James Remington

Article

Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

Insert

de4GFP

Tags

6xHis

Expression

Bacterial

Mutation

S65T/C48S/H148C/T203C

Promoter

T7

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C

Plasmid

#90014

Purpose

Split-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6C

Depositor

Julien Béthune

Article

Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

Inserts

CBirA*-Ago2
NBirA*-TNRC6C

Use

Retroviral; Flp/frt

Tags

FLAG and Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag RIOK1

Plasmid

#20543

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RIOK1 (RIOK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PBK

Plasmid

#20558

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PBK (PBK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag TEC

Plasmid

#20649

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

TEC (TEC Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag NADK

Plasmid

#20415

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

NADK (NADK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CKMT1A

Plasmid

#20459

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CKMT1A (CKMT1A Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CLK1

Plasmid

#20463

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CLK1 (CLK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
EYFP-IKB

Plasmid

#111194

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

IKB-alpha

Tags

EYFP

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pSAG1::CAS9-U6::sg290860-6

Plasmid

#59855

Purpose

Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

Depositor

David Sibley

Article

Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

Insert

CRISPR sg290860-6

Use

CRISPR; ; toxoplasma gondii

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

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XE1 Xwnt-3A

Plasmid

#16879

Depositor

Randall Moon

Article

Wolda et al Dev Biol. 1993 Jan. 155(1):46-57.

Insert

Xwnt-3A (wnt3a.L Frog)

Use

Xenopus expression

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
EF1a_MEF2C_P2A_Hygro_Barcode

Plasmid

#120458

Purpose

Barcoded lentiviral vector to express MEF2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MEF2C (MEF2C Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLK

Plasmid

#201584

Purpose

Expresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

Polymerase kappa (POLK Human)

Tags

HA

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pNM3732

Plasmid

#153086

Purpose

mec-4p GFP-C1 positive control for RMCE integration

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p GFP-C1 tbb-2 3'

Use

Rmce

Expression

Worm

Promoter

not applicable

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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ABE8.17-d

Plasmid

#136299

Purpose

expresses ABE8.17-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.20-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]

Plasmid

#71805

Purpose

Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc

Plasmid

#193346

Purpose

tetO-OS*KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + super-Sox + Klf4 + cMyc)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Pou5f1, Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

TetO mini CMV

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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