We narrowed to 18,294 results for: Cas9

1. pET28b-NmCas9-Flag

   Plasmid

   #109030

   Purpose

   Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Use

   Tags

   Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMCSG7-D16A-NmeCas9

   Plasmid

   #71476

   Purpose

   bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

   Insert

   pMCSG7-D16A-NmeCas9
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   D16A

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-Cas9-tdTomato

   Plasmid

   #141013

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMCSG7-H588A-NmeCas9

   Plasmid

   #71477

   Purpose

   bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

   Insert

   pMCSG7-H588A-NmeCas9
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   H588A

   Promoter

   T7

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_APOBEC1-UdgX-nCas9

   Plasmid

   #163531

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   APOBEC1-UdgX-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRGEB-VirD2-Cas9

   Plasmid

   #139803

   Purpose

   Expressing VirD-Cas9 fusion under ubiquitin promoter

   Depositor

   Magdy Mahfouz

   Article

   Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

   Insert

   VirD2-Cas9
   

   Use

   Tags

   VirD2-Cas9

   Expression

   Plant

   Mutation

   Promoter

   Ubiquitin

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS416gT-GalL-Cas9

   Plasmid

   #79903

   Purpose

   pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression

   Depositor

   Ronald Davis

   Article

   Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw

   Inserts

   Cas9
   guide RNA (gRNA)
   Tetracycline Repressor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPM1 promoter, GalL, and RPR1 promoter with TetO …

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ-dzCas9-NG-Act3.0

   Plasmid

   #158415

   Purpose

   CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dzCas9-NG-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. px330-CIB1-dCas9

   Plasmid

   #63665

   Purpose

   Mammalian gRNA expression vector also expressing CIB1-dCas9

   Depositor

   Dong Kong

   Article

   Chen et al Theranostics. 2018 Aug 7;8(16):4447-4461

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and CMV

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX459_gRNA pAS single_hspCas9

    Plasmid

    #193311

    Purpose

    Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    not applicable

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. G1397 DddAtox-C–dSpCas9

    Plasmid

    #157836

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221 Cas9-POLD3

    Plasmid

    #183197

    Purpose

    Entry clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    Cas9-POLD3
    

    Use

    CRISPR; Gateway entry clone

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330 NL-FKBP-Cas9

    Plasmid

    #162723

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-Flag-dSpCas9

    Plasmid

    #92113

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX335A_hCas9(D10A)_IRF8gRNA2

    Plasmid

    #89720

    Purpose

    Knockout IRF8 in human cells

    Depositor

    Martin Zenke

    Article

    Sontag et al Stem Cells. 2017 Jan 16. doi: 10.1002/st

    Insert

    IRF8 gRNA2 (IRF8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCas9–mKate2ps–CgRNA

    Plasmid

    #64955

    Purpose

    Control gRNA (GTCAAGGCACTCTTGCCTA)

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAS9-mCherry-HIST1H4C

    Plasmid

    #66944

    Purpose

    CRISPaint target selector HIST1H4C

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA HIST1H4C
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. p-dCas9-DNMT3a-Hygro

    Plasmid

    #104403

    Purpose

    transient expression of dCas9-DNMT3a fusion protein

    Depositor

    Stephan Beck

    Insert

    DNMT3a (DNMT3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSimpleII-NmCas9-FLAG

    Plasmid

    #47872

    Purpose

    FLAG tagged NmCas9 with no NLS.

    Depositor

    Erik Sontheimer, James Thomson

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Insert

    NmCas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. eSpCas9-EF-Gsk3b(new)

    Plasmid

    #122341

    Purpose

    Expresses sgRNA targeting mouse Gsk3b and eSpCas9 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Gsk3b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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