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pLVX-SVA-lncRNA-AK057321-mcherry

Plasmid

#202813

Purpose

Lentiviral vector to express of SVA-lncRNA AK057321 with CMV for mcherry expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A315T

Plasmid

#133755

Purpose

Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 315 to threonine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1_gRNA

Plasmid

#196139

Purpose

gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

AAVS1 gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-M337V

Plasmid

#133759

Purpose

Lentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed methionine 337 to valine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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LRRK2-WT

Plasmid

#17609

Depositor

Ted Dawson

Article

West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

Insert

LRRK2 (LRRK2 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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SVA-lncRNA AK057321 shRNA 1S scramble

Plasmid

#202837

Purpose

Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1S

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321 shRNA 1S scramble control

Use

Lentiviral

Expression

Mammalian

Promoter

6

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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SVA-lncRNA AK057321 shRNA 2S scramble

Plasmid

#202839

Purpose

Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 2S

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321 shRNA 2S scramble control

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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SVA-lncRNA AK057321 shRNA 1A scramble control

Plasmid

#202835

Purpose

Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1A

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

pLVX-SVA-lncRNA shRNA 1A scramble control

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-Q331K

Plasmid

#133761

Purpose

Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed glutamine 331 to lysine.

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-Bsd-CMV-hUCK

Plasmid

#195141

Purpose

UCK2 overexpression vector with blasticidin resistance

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

UCK2 (UCK2 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-K263E

Plasmid

#133758

Purpose

Lentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed lysine 263 to glutamate.

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Chr1q_Centromere-Targeting_gRNA

Plasmid

#195125

Purpose

gRNA targeting centromere-proximal location on Chromosome 1q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-JAM2-SVA

Plasmid

#202832

Purpose

pGL3 luciferase reporter with JAM2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

JAM2 intronic SVA

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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Chr7_Centromere-Targeting_gRNA

Plasmid

#195129

Purpose

dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr7 gRNA

Expression

Mammalian

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.1

Plasmid

#91895

Purpose

sgNf1.1 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.1

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.4

Plasmid

#92029

Purpose

sgNf1.4 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.4 for Nf1 deletion

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A90V

Plasmid

#133754

Purpose

Lentiviral expression of human TDP-43 A90V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 90 to valine

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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Chr8q_Centromere-Targeting_gRNA

Plasmid

#195128

Purpose

gRNA targeting centromere-proximal location on Chromosome 8q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr8q gRNA

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-P112H

Plasmid

#133760

Purpose

Lentiviral expression of human TDP-43 P112H disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed proline 112 to histidine.

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Rosa26_gRNA

Plasmid

#196138

Purpose

gRNA targeting the Rosa26 locus in a thid generation Cas9 backbone with TagBFP2

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Rosa26 gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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