We narrowed to 10,911 results for: aga

1. pSCL.177

   Plasmid

   #184998

   Purpose

   Express -Eco1 EMX1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Use

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   EMX1 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. NSP3C-Flag

   Plasmid

   #210349

   Purpose

   Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   C terminal of SARS-CoV-2 NSP3 (ORF1ab )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-HA-UBE3B(C1036A)-IRES-Puro

   Plasmid

   #107348

   Purpose

   Expresses HA tagged UBE3B mutant C1036A in mammalian cells.

   Depositor

   Robert Sobol

   Article

   Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

   Insert

   UBE3B(C1036A) (UBE3B Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed Cysteine 1036 to Alanine

   Promoter

   CMV

   Available since

   April 19, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pMSCV-IRES-GFP-TLR4 delta tail

   Plasmid

   #133063

   Purpose

   Retroviral expression of mouse TLR4 with out its cytosolic tail

   Depositor

   Jonathan Kagan

   Article

   Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

   Insert

   TLR4 delta tail (Tlr4 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRAF6mut-UTR

   Plasmid

   #15094

   Depositor

   David Baltimore

   Article

   Taganov et al Proc Natl Acad Sci U S A. 2006 Aug 15. 1

   Insert

   TRAF6 3'UTR (TRAF6 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   4-bp substitutions in the miR-146 target sites

   Promoter

   Available since

   June 6, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pMSCV-IRES-GFP-CD4-TLR4 cyto

   Plasmid

   #133064

   Purpose

   Retroviral expression of mouse CD4 extracellular domain fused with the cytosolic tail of TLR4

   Depositor

   Jonathan Kagan

   Article

   Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

   Insert

   CD4-TLR4cyto (Tlr4 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTBL437 AAVS1 EQR sgRNA

   Plasmid

   #126447

   Purpose

   To cut the human AAVS1 locus for integration.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human AAVS1 safe-harbor locus with NGAG PAM
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSMP-EZH1_2

   Plasmid

   #36360

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   EZH1 (EZH1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

9. HA-HIF2α MT-pBabe-Hygro

   Plasmid

   #22134

   Depositor

   William Kaelin

   Article

   Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA "silent"("conservative"…

   Promoter

   Available since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-TAC-OKMS-Cas9

    Plasmid

    #120353

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OKMS cassette
    

    Use

    CRISPR; Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Promoter

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET29a-hNABP1-C

    Plasmid

    #128311

    Purpose

    Express hNABP1 C-terminus (125–204 aa) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human nucleic acid-binding protein 1 (hNABP1)-C terminus (125-204 aa) (NABP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CMV-cGASΔN-HA (murine)

    Plasmid

    #130915

    Purpose

    Expresses murine cGASΔN (aa148-507)-HA; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    cGASΔN murine
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    murine cGAS catalytic domain (aa148-507)

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-IRES-GFP-Hybrid-caspase_human-caspase-4-CARD+canine-Caspase-1/4-catalytic domain

    Plasmid

    #183397

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    CARD domain of human caspase-4 fused to catalytic domain of canine caspase-1/4
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSV CDK9 HA (P#598)

    Plasmid

    #14638

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

    Insert

    cdk9 (CDK9 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-UBE3B-BirA-Puro

    Plasmid

    #107352

    Purpose

    Expresses UBE3B fused to BirA in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    UBE3B-BirA (UBE3B Human)
    

    Use

    Lentiviral

    Tags

    Biotin ligase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 23, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pDsRed-HP1alpha

    Plasmid

    #179702

    Purpose

    visualization of centromere heterochromatin

    Depositor

    Kenji Sugimoto

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2014;78(4):55

    Insert

    heterochromatin protein alpha (CBX5 Human)
    

    Use

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CMV-cGAS-N-HA (murine)

    Plasmid

    #130916

    Purpose

    Expresses murine cGAS N terminus(aa1-147)-HA; Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    murine cGAS N terminus
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    murine cGAS N terminus (aa 1-147)

    Promoter

    CMV

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO-shAUF1 hairpin 1

    Plasmid

    #52921

    Purpose

    expresses a hairpin specific for human AUF1

    Depositor

    Sheila Stewart

    Article

    Alspach et al Cancer Discov. 2014 Mar 26.

    Insert

    shRNA targeting AUF1 (HNRNPD Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pLKO-shAUF1 hairpin 2

    Plasmid

    #52922

    Purpose

    expresses a hairpin specific for human AUF1

    Depositor

    Sheila Stewart

    Article

    Alspach et al Cancer Discov. 2014 Mar 26.

    Insert

    shRNA targeting AUF1 (HNRNPD Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pZLCv2-gMYOD1-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106355

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of MYOD1 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    MYOD1 gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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