We narrowed to 5,840 results for: pCas

1. pCAG-HIF1 alpha K532R

   Plasmid

   #21102

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   HYPOXIA INDUCIBLE FACTOR 1 ALPHA-K532R MUTANT (HIF1A Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 6, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pSLQ5135: pCAG-BFP-GuideArray

   Plasmid

   #210606

   Purpose

   Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

   Depositor

   Stanley Qi

   Article

   Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

   Insert

   pCAG-BFP-GuideArray
   

   Use

   CRISPR

   Promoter

   CAG

   Available Since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cmv d0 dPspCas13b-GGS-Sun

   Plasmid

   #119858

   Purpose

   Cmv d0 dPspCas13b-GGS-Sun no-fusion construct

   Depositor

   Bryan Dickinson

   Article

   Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

   Insert

   dCas13b-GGS-Sun
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-postASAP-p2a-ChrimsonR

   Plasmid

   #178794

   Purpose

   Expresses voltage sensor targeted to dendrites and spines along with ChrimsonR

   Depositor

   Rafael Yuste

   Article

   Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

   Insert

   FingR.PSD95, ASAP2f and ChrimsonR
   

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   mutations in amino acids L146G, S147T N149R, S150…

   Promoter

   CAG

   Available Since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-GBP6-10gly-VPminx4

   Plasmid

   #50796

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GBP6-VPminx4 activation domain fusion protein
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-VPR-dSpCas9[StaPL(TI)]

   Plasmid

   #111508

   Purpose

   Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].

   Depositor

   Michael Lin

   Article

   Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

   Insert

   VPR-dSpCas9[StaPL(TI)]
   

   Expression

   Mammalian

   Mutation

   The HCV NS3 protease carries F43L, Q80K, and S122…

   Promoter

   PGK

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-FLAG-CaMKIID-K43M

   Plasmid

   #186830

   Purpose

   Expresses FLAG-CaMKIID-K43M in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   Camk2d (Camk2d Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Lysine 43 to Methionine

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-SARS2-S-D614 (NC-flag)

   Plasmid

   #156418

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Michael Farzan, Hyeryun Choe

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-B-eSpCas9-NLS-6xHis

   Plasmid

   #207383

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A, amino acids 1005-1013 repl…

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-ChroME(S273A)-mRuby2-ST

    Plasmid

    #109128

    Purpose

    Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChroME(S273A)-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAX APPs-751-alpha

    Plasmid

    #30149

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein - soluble (APP Human)
    

    Expression

    Mammalian

    Mutation

    splice variant 751; amino acids 1–612

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pCAG-MCS-BioID2-3xFLAG

    Plasmid

    #186812

    Purpose

    CAG promoter-driven expression of BioID2-fused protein (C terminus)

    Depositor

    Masahito Ikawa

    Article

    Oura et al Sci Rep. 2022 Dec 23;12(1):22198. doi: 1

    Type

    Empty backbone

    Tags

    BioID2-3xFLAG

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid

    #98975

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 4 and Cas9(D10A)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-mTagBFP2-T2A-dre

    Plasmid

    #124832

    Purpose

    Monomeric TagBFP2 and dre recombinase

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-dre
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-2dV-Camui (T286A)

    Plasmid

    #220367

    Purpose

    T286A phosphodead mutant of pCAG-2dV-Camui

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T286A)-mEGFP(A206K) (Camk2a Rat, Synthetic)
    

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Alanine

    Promoter

    CAG

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-hPhyB621-mCherry-HRasCT

    Plasmid

    #100282

    Purpose

    Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (1-621 aa) (PHYB Mustard Weed)
    

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-621 aa)

    Promoter

    CAG promoter

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Osa-pCAMBIA-1301-STTM319

    Plasmid

    #84201

    Purpose

    Disturb the expression of miR319 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR319
    

    Expression

    Bacterial, Plant, and Yeast…

    Promoter

    2x35S

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-FLAG-SpCas9-HF1

    Plasmid

    #126770

    Purpose

    Expression of increased fidelity SpCas9-HF1 in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-pac-T2A-dre

    Plasmid

    #124833

    Purpose

    Puromycin resistance gene and dre recombinase

    Depositor

    Mario Capecchi

    Insert

    pac-T2A-dre
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-nDsRedFL-miR9/9*/124

    Plasmid

    #177807

    Purpose

    The pre-miR-9/9* and pre-miR-124 sequences have been inserted into the EcoRV of pCAG-nDsRedFL-intron.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    miR-9/9*/124
    

    Expression

    Mammalian

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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