Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 15,064 results for: pET

Showing: 4301 - 4350 of 15064 results

  9. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

    Plasmid
    #153441
    Purpose
    E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc. XDocB binding mode B knock-out mutant
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Arginine 140 and methionine 144 were mutated to a…
    Promoter
    T7 promoter
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153441-plasmid-map-sequence-id-306441

    Enlarge

  10. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)

    Plasmid
    #153440
    Purpose
    E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc. XDocB binding mode A knock-out mutant
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Arginine 191 and leucine 195 on XDocB were mutate…
    Promoter
    T7 promoter
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153440-plasmid-map-sequence-id-306440

    Enlarge

  27. pET-28b-T7-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-6xHis (AAS1880)

    Plasmid
    #114071
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a(E174R/S542R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (E174R/S542R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R and S542R
    Promoter
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114071-plasmid-map-sequence-id-220509

    Enlarge

  31. pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His

    Plasmid
    #222288
    Purpose
    Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.
    Depositor
    Anne Straube
    Article
    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-
    Insert
    KIF1C (KIF1C Human)
    Use
    Tags
    6xHis, GFP, and GST
    Expression
    Bacterial
    Mutation
    stalk region only amino acids 642 to 922
    Promoter
    T7
    Available Since
    June 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    222288-plasmid-map-sequence-id-475373

    Enlarge

  32. pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (I224A/L227A/W276A/I279A; deltaLIR1+3)

    Plasmid
    #223754
    Purpose
    Expression of recombinant protein for purification
    Depositor
    Sascha Martens
    Article
    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038
    Insert
    BCL2L13 soluble part (BCL2L13 Human)
    Use
    Tags
    Thrombin cleavage-GST
    Expression
    Bacterial
    Mutation
    expresses aa 1-465, with I224A/L227A/W276A/I279A;…
    Promoter
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    223754-plasmid-map-sequence-id-448302

    Enlarge

  33. pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A/I307A/V310A; deltaLIR1+4)

    Plasmid
    #223755
    Purpose
    Expression of recombinant protein for purification
    Depositor
    Sascha Martens
    Article
    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038
    Insert
    BCL2L13 soluble part (BCL2L13 Human)
    Use
    Tags
    Thrombin cleavage-GST
    Expression
    Bacterial
    Mutation
    expresses aa 1-465, with W276A/I279A/I307A/V310A;…
    Promoter
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    223755-plasmid-map-sequence-id-448303

    Enlarge

  34. pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (Y213A/I216A/W276A/I279A; deltaLIR1+2)

    Plasmid
    #223752
    Purpose
    Expression of recombinant protein for purification
    Depositor
    Sascha Martens
    Article
    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038
    Insert
    BCL2L13 soluble part (BCL2L13 Human)
    Use
    Tags
    Thrombin cleavage-GST
    Expression
    Bacterial
    Mutation
    expresses aa 1-465, with Y213A/I216A/W276A/I279A;…
    Promoter
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    223752-plasmid-map-sequence-id-448281

    Enlarge

  35. pETDuet1_NIX(1-182aa)-THROMBIN-GST (E72A/L75A/D77A/E81A; deltaWIPI2)

    Plasmid
    #223753
    Purpose
    Expression of recombinant protein for purification
    Depositor
    Sascha Martens
    Article
    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038
    Insert
    NIX (BNIP3L) soluble part (BNIP3L Human)
    Use
    Tags
    Thrombin cleavage-GST
    Expression
    Bacterial
    Mutation
    expresses aa 1-182, with E72A/L75A/D77A/E81A; del…
    Promoter
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    223753-plasmid-map-sequence-id-448300

    Enlarge

  36. pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

    Plasmid
    #180806
    Purpose
    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrep
    Depositor
    Enfu Hui
    Article
    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Insert
    BTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep (BTLA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    BTLA (Y243F, Y257F, Y282F)
    Promoter
    T7
    Available Since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180806-plasmid-map-sequence-id-356797

    Enlarge

  37. pET28-His10-BTLAicd (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

    Plasmid
    #180807
    Purpose
    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y257F, Y282F)-TwinStrep
    Depositor
    Enfu Hui
    Article
    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Insert
    BTLAint (AA190-289, Y226F, Y257F, Y282F)-TwinStrep (BTLA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    BTLA (Y226F, Y257F, Y282F)
    Promoter
    T7
    Available Since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180807-plasmid-map-sequence-id-356795

    Enlarge

  38. pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y282F)-TwinStrep

    Plasmid
    #180808
    Purpose
    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y282F)-TwinStrep
    Depositor
    Enfu Hui
    Article
    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Insert
    BTLAint (AA190-289, Y226F, Y243F, Y282F)-TwinStrep (BTLA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    BTLA (Y226F, Y243F, Y282F)
    Promoter
    T7
    Available Since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180808-plasmid-map-sequence-id-356794

    Enlarge

  39. pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y257F)-TwinStrep

    Plasmid
    #180809
    Purpose
    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y257F)-TwinStrep
    Depositor
    Enfu Hui
    Article
    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Insert
    BTLAint (AA190-289, Y226F, Y243F, Y257F)-TwinStrep (BTLA Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    BTLA (Y226F, Y243F, Y257F)
    Promoter
    T7
    Available Since
    March 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    180809-plasmid-map-sequence-id-356793

    Enlarge

  40. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (wild type)

    Plasmid
    #153439
    Purpose
    E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Article
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.XDocB
    Use
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available Since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    153439-plasmid-map-sequence-id-306439

    Enlarge

  41. pET-28b-T7-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1916)

    Plasmid
    #114076
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RR (S542R/K607R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R and K607R
    Promoter
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114076-plasmid-map-sequence-id-220531

    Enlarge

  45. pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

    Plasmid
    #117006
    Purpose
    Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    117006-plasmid-map-sequence-id-227036

    Enlarge

  46. pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

    Plasmid
    #114072
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, and K548R
    Promoter
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114072-plasmid-map-sequence-id-220522

    Enlarge

Showing: 4301 - 4350 of 15064 results