We narrowed to 2,540 results for: T2A

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pPB-TetON-mEGFP-NGN2_AroPERFECT

Plasmid

#215612

Purpose

For integration of NGN2 AroLITE T2A mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NGN2 AroLITE (NEUROG2 Human)

Tags

T2A mEGFP

Expression

Mammalian

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

Plasmid

#202553

Purpose

Nontargeting control vector with TagBFP2

Depositor

Frederic Meunier

Article

Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

Insert

Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))

Use

CRISPR and Lentiviral

Tags

3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

Mutation

Puromycin-resistance gene in the pLV hU6-sgRNA hU…

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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tetO-GFPnls-mPGK-rtTA-IRES-Puro

Plasmid

#69545

Purpose

Tet-On system expressing GFP tagged with 2x nuclear localization signals.

Depositor

Charles Gersbach

Insert

eGFP

Use

Lentiviral

Tags

2x nuclear localization sequence and T2A "sk…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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lenti-dCas9-VP64-BFP

Plasmid

#196711

Purpose

CRISPR-activation. dCas9-VP64 with TagBFP for sorting

Depositor

Bernhard Schmierer

Insert

dCas9-VP64-T2A-TagBFP

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Mutation

D10A, N863A

Promoter

EF1a

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE3G-dCas9-VP64-MS2-VP64-ZsG

Plasmid

#192669

Purpose

Dox-inducible expression of dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

Depositor

Jeffrey Miner

Insert

dCas9-VP64, MS2-VP64, ZsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

TRE3G

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VPR-Puro

Plasmid

#242162

Purpose

Lentiviral expression of dCas9-VPR with puromycin reporter

Depositor

Amy Brock

Insert

dCas9-VP64-RelA-Rta; T2A-puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VP64-H2B-GFP

Plasmid

#242164

Purpose

Lentiviral expression of dCas9-VP64 with H2B-GFP reporter

Depositor

Amy Brock

Insert

dCas9-VP64; T2A-H2B-GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VP64-H2B-mCherry

Plasmid

#242165

Purpose

Lentiviral expression of dCas9-VP64 with H2B-mCherry reporter

Depositor

Amy Brock

Insert

dCas9-VP64; T2A-H2B-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-KRAB-H2B-mCherry

Plasmid

#242167

Purpose

Lentiviral expression of dCas9-KRAB with with H2B-mCherry reporter

Depositor

Amy Brock

Insert

dCas9-KRAB; T2A-H2B-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-KRAB-H2B-GFP

Plasmid

#242166

Purpose

Lentiviral expression of dCas9-KRAB with H2B-GFP reporter

Depositor

Amy Brock

Insert

dCas9-KRAB; T2A-H2B-GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-FnCas9

Plasmid

#130969

Purpose

3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

Insert

FnCas9

Use

CRISPR

Tags

3xHA-NLS and NLS-T2A-EGFP

Expression

Mammalian

Promoter

Cbh

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-SpCas9

Plasmid

#130968

Purpose

3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

Insert

3xHA-NLS-SpCas9

Use

CRISPR

Tags

3x HA, EGFP, and NLS

Expression

Mammalian

Promoter

Cbh

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

Plasmid

#192666

Purpose

3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker

Depositor

Jeffrey Miner

Insert

dCas9-VP64, MS2-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR.v2.TetON_Cas13d_U6_DR

Plasmid

#196727

Purpose

Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.

Depositor

Bernhard Schmierer

Insert

RfxCas13d-T2A-miRFP670nano

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Promoter

TRE

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-SAGFP

Plasmid

#127100

Purpose

SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.

Depositor

Adam Karpf

Insert

SA-GFP reporter

Expression

Mammalian

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

Plasmid

#192665

Purpose

3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1

Depositor

Jeffrey Miner

Insert

dCas9-VP64, MS2-VP64, ZsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VPR-H2B-GFP

Plasmid

#242168

Purpose

Lentiviral expression of dCas9-VPR with H2B-GFP reporter

Depositor

Amy Brock

Insert

dCas9-VP64-p65/RelA-Rta; T2A-H2B-GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-KRAB-MeCP2-Puro

Plasmid

#242163

Purpose

Lentiviral expression of dCas9-KRAB-MeCP2 with puromycin reporter

Depositor

Amy Brock

Insert

dCas9-KRAB-MeCP2; T2A-puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VPR-H2B-mCherry

Plasmid

#242169

Purpose

Lentiviral expression of dCas9-VPR with H2B-mCherry reporter

Depositor

Amy Brock

Insert

dCas9-VP64-p65/RelA-Rta; T2A-H2B-mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-KRAB-MeCP2-H2B-GFP

Plasmid

#242170

Purpose

Lentiviral expression of dCas9-KRAB-MeCP2 with H2B-GFP reporter

Depositor

Amy Brock

Insert

dCas9-KRAB-MeCP2; T2A-H2B-GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 2, 2026

Availability

Academic Institutions and Nonprofits only

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